AIM: To investigate pharmacdogical mechanism of Negundo Chastetree extract liquor (NCEL), the effect of (NCEL) on blood-fat, liver-fat and blood-sugar in the rat and the mouse were studied in this paper. METHODS: The experimental models were established by giving rats and mice NCEL orally at dosages 5.0, 7.5, 10.0 mL/kg.b.w respectively. Then the level of blood-fat, liver-fat, and blood-sugar were observed. RESULTS: The level of serum triglyceride and total cholesterol decreased considerably in mice, at the same time, the increase of triglyceride induced by high lipid diet in liver of mice were inhibited significantly. In addition, the level of fatty liver of hepatic homogenization triglyceride in rats with induced by DL-ethionine was lowered obviously by oral administration of NCEL. CONCLUSION: These results suggest that NCEL may have effects of reducing blood-fat and pretecting liver.