Near infrared spectra in the range of 11501.4 ～ 6047.7 cm- 1 were used for simultaneously determination four oestrogens and progestogens: estradiol, estriol, medroxyprogesterone acetate and estrone. Constant offset elimination data pretreatment and partial least square regression method were used to resolve the spectra overlap of the four substancesas well as the tetrahydrofuran solvent. 100 samples were taken as the calibration set getting a correlation coefficient of 0. 99. The relative deviations for prediction samples were < 5 %. Some measuring factors influencing regressive accuracy like moistrue, temperature and dissolvent were discussed.

Objective : To study the mechanism of neurotrophin receptor⁃interacting MAGE homolog (NRAGE) on the proliferation and invasion of colorectal cancer (CRC) cells. Methods : The clinicopathological data of 84 CRC patients were selected. Fluorescence quantitative PCR (qPCR) and Western blot were used to detect the expression of NRAGE in CRC tissues and adjacent normal tissues. The relationship between the expression of NRAGE in cancer tissues and clinicopathological characteristics was analyzed statistically. RT⁃PCR and Western blot were used to detect the expression of NRAGE mRNA and protein in CRC tumor cell lines HT29 , SW480 , SW620 , LOVO and colorectal normal cell line FHC. MTT proliferation experiment and Transwell migration experiment were used to observe the tumor cell proliferation and migration ability of the NC group , overexpression NRAGE group and NRAGE knockdown group. The expression differences of AKT , p ⁃AKT , ERK1/2 , p ⁃ERK1/2 , E ⁃cadherin , N ⁃cadherin and Vimentin were detected by qPCR and Western blot. Results : Compared with adjacent tissues , NRAGE mRNA and protein expression in CRC cancer tissues were significantly higher. The expression of NRAGE in cancer tissues was related to tumor stage , distant metastasis and lymph node metastasis (all P < 0. 05) . Compared with FHC cells , CRC tumor cell lines HT29 , SW480 , SW620 , and LOVO cells had higher NRAGE mRNA and protein expression (P < 0. 05) . Compared with the NC group , the proliferation and migration ability of CRC tumor cells in the overexpression NRAGE group was significantly enhanced , while the knockdown group was significantly weakened. Compared with the NC group , the SW480 cells in the overexpression NRAGE group had higher p ⁃ERK1/2 protein expression , but there was no significant difference in the expression of ERK1/2 , AKT and p ⁃AKT. After the SW480 cells in the overexpression NRAGE group were treated with ERK inhibitor U0126 , the proliferation and migration ability of SW480 cells significantly reduced. Methods NRAGE can promote the epithelial⁃mesenchymal transition of CRC cells and enhance the proliferation and migration of CRC tumor cells by activating ERK signaling pathway.