Objective To establish a Taqman real-time PCR assay for quantitative detection of the expression of metabolic syndrome related gene (MSRG) mRNA, and to study the function of a new gene. Methods Specific primers and probes were designed for real-time PCR according to the MSRG cDNA sequence. The plasmid standard preparations were constructed by T-A clone, and serial 10-fold dilutions of the extracted plasmid standard preparations were prepared for plotting the standard curve which was used for relative quantification of real-time PCR. The sensitivity, specificity and reproducibility of real-time PCR assay were detected. To compare with the semi-quantitative RT-PCR, the expression levels of MSRG were measured by real-time PCR and semi-quantitative RT-PCR, respectively, in HepG2 cells which were incubated with glucose in different concentrations [5.6mmol/L (G5.6), 22mmol/L (G22) and 33.3mmol/L(G33.3)]. Results An effective real-time PCR assay was established for detection of MSRG mRNA expression levels. Significant differences existed in MSRG expression in HepG2 cells between the G33.3, G22 and G5.6 groups detected by real-time PCR assay. The expression levels of MSRG in HepG2 cells increased significantly in G33.3 group, whereas no significant difference on the expression level of MRSG mRNA was found between G22 and G5.6 groups when semi-quantitative RT-PCR was used for detection. It suggested that the real-time PCR assay was more sensitive and even more precise than that of semi-quantitative RT-PCR. Conclusion The real-time PCR assay was a sensitive, specific, quantitative, and reproducible tool for studying the function of MSRG at the mRNA expression levels of gene.

The article analyzes the current existing problems in bilingual teaching,and accordingly brings forward some possible methods to solve the problems and improve the teaching quality.

In this paper the basic feature of the participating teaching approach is elaborated,and the problems needing to be paid attention to in the process of its application and fulfillment are also discussed.

Problem based learning(PBL) is a new teaching method that centers on the problems of patients' diseases,the learning of students,guided by the instructors,but students positive.However,nowadays,it can not be completely implemented in our nation.We should choose the combination of the traditional and innovatory methods,and execute the reformation gradually.

Laboratory education in basic medicine plays a significant part in medical education.By investigating and analyzing the recent laboratory education in basic medicine,it was found out that the teaching effect of recent laboratory education was basically appropriate.But there was still some deficiency in cultivating problem-solving capability and innovation thinking.

To briefly introduce the basic course of training,practice and lecture for volunteers in AIDS Youth Companion Education Activity held by Melbourne University of Australia and School of Public Health of Sichuan University,and to discuss the existing problems in the Companion Education as well as personal experiences.

The advantages and disadvantages of traditional method and "PBL"(Problem-Based Learning) in Basic Medicine education are analyzed.Based on the analysis above,a new method which possesses the advantages and discards the disadvantages is introduced to adapt to the education in Basic Medicine in China and will be applied to the laboratory education.

In this paper some suggestions of morphologic experimental teaching methods were put forward.

Objective To study the effects of ?2-adrenergic receptor gene Arg16Gly polymorphism on blood lipid and apolipoprotein ratio and its role in blood lipid and apolipoprotein ratio mediated by high carbohydrate/low fat (HC/LF) diet in healthy young persons.Methods Fifty-six healthy young volunteers had regular diet for 7 d followed by HC/LF diet for 6 d.Twelve-hour fasting venous blood samples were collected on days 1,8 and 14 to measure blood lipid and apolipoprotein (apo) AI and B100 levels,and to calculate ratios of TG/HDL-C,log (TG/HDL-C),TC/HDL-C,LDL-C/HDL-C and apoAI/apoB100.DNA was isolated from genome.Arg16Gly polymorphism was analyzed by PCR-RFLP.Results No significant difference was found in the baseline lipid and apolipoprotein ratio in subjects with AA genotype and G carriers before and after regular or HC/LF diet.The ratios of TG/HDL-C (P=0.017),log (TG/HDL-C) (P=0.031),and apoAI/apoB100 (P=0.006) were significantly higher,while those of TC/HDL-C (P=0.001) and LDL-C/HDL-C (P