Carotid atheroscleorotic stenosis is one of the most common causes of cerebral ischemic stroke. In China, unfortunately, Its importance has not been fully stressed,and carotid endarterectomy hasn't been used widely so far. Therefore, noninvasive studies on carotid atheroscleorotic stenosis are introduced. The surgical and intravascular interventional therapies are emphasized again.

Objective To observe the expression of neuroglobin (Ngb) in cerebral cortex of traumatic brain injury rats, and investigate the role of neuroglobin in brain injuries. Methods A traumatic brain injury rat model was constructed with Feeney's method. Fifiteen experiment animals were divided randomly into normal control group, 12h-post-trauma group and 36h-post-trauma group (5 each). Immunohistochemistry and image analysis techniques were used to measure the area, optic density (OD) value and integrated optic density (IOD) value of Ngb immunoreactive products in primary somatosensory cortex (S1) and retrosplenial granular b cortex (RSGb), which was located in the inner side and out side of traumatic brain injury cavity in coronal section of rat brain respectively. Results Ngb immunoreactive products were significantly increased after brain trauma, especially in 36h-post-trauma group. The image analysis showed that there was no significant difference of immunoreactive product area in the 3 groups (P

Objective to study the effect of blood pressure on the enlargement of saccular aneurysm and its mechanism. Methods Forty-eight SD rats were divided into 3 groups randomly(A, B and C; n=16 in each group). Saccular aneurysms of the carotid artery were induced by corrosion of the initial segment of the external carotid artery (1mm long) with elastase. In group A, the posterior branches of both renal arteries were ligated in order to produce a marlced blood pressure elevation. In group B, only unilateral renal artery was ligated, and a moderate hypertension was produced. In group C high blood pressure was not produced to serve as the control group. After 2 and 8 weeks, the blood pressure and the size of the aneurysms were measured. Then the aneurysms were fixed by intraarterial perfusion of formalin and were harvested for examinations. The expression of basic fibroblast growth factor (bFGF) in aneurismal wall was determined by immunohistochemical analysis. Results Two weeks after the beginning of the experiment, there was no difference in size of aneurysm among the 3 groups. But after 8 weeks, significant difference in aneurysmal size was found between group A and the other two groups. The expression of bFGF in group A was stronger than that in group B and C. Conclusion The elevation of blood pressure and enhancement of bFGF expression in aneurismal wall play an important role in enlargement of the aneurysm.

Objective To study the advantages and procedure of telovelar approach for resection of the lesion in the fourth ventricle. Methods Telovelar approach to the fourth ventricle was executed in 17 cases. Among these patients, seven were females and 10 were males. The age ranged from 5 years to 49 years with mean age of 26.6 years. Pathologically, there were 5 medulloblastomas, 5 ependymomas, 2 epidermoids, 2 brain cysticercosis, and 3 choroid plexus papillomas. During the operation, the medullotonsillar space of the cerebellomedullary fissure and the urulotonsillar space were sharply dissected to release the tonsils from uvula and medullar oblongata bilaterally. According to the size of the neoplasm, the tela or both tela and velum were incised. The fourth ventricle was exposed adequately and the lesion was removed. All operations were performed with the help of microscope. The dura was sutured securely. Results The space occupying lesions were removed totally in all cases and all patients recovered very well, and threre was no mortality or serious disability. With 3 to 21-month follow-up in 11 cases, no death or serious disability was found, and the CT or MRI in 8 patients showed no recurrence. Conclusion The telovelar approach can provide adequate exposure to remove the lesion in the fourth venticle. The inferior vermis need not be incised, so a minimum amount of brain tissue was injured, and postoperative complication is decreased.

Objective To study the role of VEGF on the growth of hypertensional saccular aneurysm and its mechanism. Methods Twenty-four SD rats were divided into 2 groups randomly (A and B, each with 12 rats). Saccular aneurysms of the carotid artery were reproduced by treatment of initial segment of the external carotid artery (1mm long) with elastase. In group A, the posterior branches of both renal arteries were ligated in order to produce marked elevation of blood pressure. In Group B hypertension was not produced to serve as the control group. After 8 weeks, the blood pressure and the size of the aneurysms were measured. Then formalin was infused into the arteries for fixation, and aneurysms were resected. The expression of vascular endothelial growth factors (VEGF) in aneurysmal wall was assessed with immunohistochemical analysis. Results The size of aneurysms: after 8W, significant difference was found between A and B group (P

s Objective To observe the changes in the expression of ?-amyloid precursor protein (?-APP) in selected brain areas in early stage of diffuse axonal injury (DAI) in rats. Methods DAI was induced in SD rats by a self-made rotating injury device. 16 rats in injury group were sacrificed by decapitation at 3 hours and 6 hours post-trauma, while the other 8 rats in sham injury group were put to death after 24 hours. The samples of the rat brain were stained with ?-APP for immunohistochemistry in order to detect the early stage pathological changes in subcortex white matter, hippocampus, corpus callosum and brain stem at different time points. Results The ?-APP expression was positive at 3 hours and was particularly strong at 6 hours after injury. The results of semi-quantitative analysis showed a significant differentiation of 3 hours and 6 hours in the immuno-positive expression of brain stem, corpus callosum and hippocampus. Histological results demonstrated that DAI occurred in all rats of injury group. Conclusion ?-APP is a kind of sensitive and useful immunochemical marker for early DAI diagnosis, and it indicates axonal lesion or breakage.

Objective To observe the dynamic changes in neuroglobin (NGB) expression in cerebral cortex and hippocampus after cerebral ischemia-reperfusion injury in gerbils. Methods The global cerebral ischemia of gerbils was induced by clamping bilateral carotid arteries for 20 minutes, then they were released to allow reperfusion for 2h, 8h, 16h, 24h, 48h and 72h. The NGB expression in cerebral cortex and hippocampus was measured with immunohistochemical methods coupled with computer-assisted image analysis. Results The changes in NGB expression were different in cerebral cortex and in the hippocampus. NGB protein was up-regulated from 16h to 48h after ischemia-reperfusion insult to cerebral cortex, whereas it was down-regulated hippocampus. Conclusion The different expressions of NGB in cerebral cortex and hippocampus are suggestive of compensatory and repair mechanisms in ischemia-damaged neurons after transient global cerebral ischemia. The increased expression of endogenous NGB in the brain after ischemia-reperfusion may be associated with the protective response to ischemic damage.

Objective To observe the inhibit effect of triplex forming oligonucleotide (TFO)(platelet-derived growth factor-B chain, PDGF) on tumor growth and angiogenesis in rats with glioma.Methods 1?10~6 C_6 glioma cells with high-flow microinfusion were seed into right caudate putamens of 18 rats by stereotaxic technique. TFO was injected in situ 1 week after glioma cells inoculation. Treat group Ⅰ and treat group Ⅱ received TFO at dose of 1.5 mg/20 ?l and 3.0 mg/20 ?l, respectively. The same doses were given again at 8, 11 and 14th day after glioma cells inoculation. The control group was treated with 20 ?l normal saline at same time like treat groups. Three weeks after glioma cells inoculation, all the rats were killed. The expressions of, PDGF-B, vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) were detected with microscopic histology.Results The inhibition rate of tumor growth was 66.0% in treat groupⅠand 92.2% in treat group Ⅱ. There was significant difference between the two groups ((P

The aim was to look for the method for getting highly purified population and large amount of Schwann cells from the newborn rats. Sciatic nerves from 1～3 days old SD rats were taken out . The Schwann cells were cultured and purified by modified repeated explanation, and fibroblastic cells were removed by rapid trysinization , differential adhesion and anti mitosis methods. The Schwann cells were identified by indirect immunocytochemistry with mouse anti S 100 protein McAb. The result showed that the purity of Schwann cells was more than 95%, and the cells were in a good state. It is considered that the methods are simple and convenient , and a great number of highly purified Schwann cells can be acquired by them.

To correlate the ultrasound and MR imaging of carotid atherosclerotic plaque with its histologic compositions,20 patients(22 lesions) scheduled for carotid endarterectomy were examined by B mode ultrasound and MRI preoperatively. The specimens were also examined postoperatively and the images of carotid plaque were recorded. The transverse pathological sections of plaques were made and matched with images of ultrasound and MRI. 256 gray scale ultrasound densitometric analysis of carotid plaque compositions was performed. Ultrasonic density of calcium, fibrous tissue, hemorrage/thrombus and lipid deposits was (89?12), (53?8), (37?6) and (39?3) respectively in vivo , and was (168?11), (136?12), (85?12) and (89?10) respectively in vitro . In vivo relative signal intensities of calcium, fibrous tissue, old hemorrhage/thrombus and lipid deposits were low, equal, equal and very high respectively on T 1 W MR image, and very low, equal, high and high respectively on PDW MR image, and low, equal or slightly high, high and high respectively on T 2 W MR image. Signal intensities of plaque contents ex vivo were correlated to signal intensities in vivo. Densitometric analysis of ultrasound images of carotid plaques can quantify possible components of plaque, and MRI has the feasibility to identify the components of plaque.