To evaluate whether different doses of X ray irradiation can induce apoptosis of rat C6 glioma cells. A rat C6 glioma cell was irradiated with X ray in different doses in vitro. Apoptosis was identified by the classical “ladder” pattern (oligonucleosome sized fragments) in DNA agarose gel electrophoresis. Results: The results showed that significant apoptosis was found in 15, 20 or 30 Gy groups at 4 hours and 1 day after X ray irradiation compared with control, whereas this phenomenon could only be seen vaguely in the 40 Gy group. The latter group showed more necrosis than apoptosis. It suggested that the apoptotic mode of cell death and necrosis may represent the response in this X ray irradiated rat C6 glioma cells in vitro.

Fourteen patients with vascular stenosis in carotid artery system were treated using angioplasty and stenting.The stenosis was located at bifurcation in 6 cases, intracranial internal carotid artery in 3, horizontal segment of middle cerebral artery in 3, and original segment of common carotid artery in 2. Depending on the long and degree of stenosis,different types of SMART stents and Biodivysio stents were selected for the endovascular angioplasty via the femoral approach in 13 cases. Balloon dilatation angioplasty was performed in one case. All the angioplasties in 14 cases were successfal. Different improvement was achieved in patients with neurological defects and no stroke was found in NIHSS after endovascular angioplasty. Following up 3 to 14 months, there was no TIA and other ischemic neurological defect. The primary results of our group showed that endovascular angioplasty and stenting are effective and safe for carotid stenosis. It should be one of the favorable choices for the treatment of vascular stenosis in catotid artery system.