Objective To explore the effect of rehabilitation training on negative symptom of patients with schizophrenia. Methods 70schizophrenics were divided into control group (n=35) and observation group (n=35). Both groups took antipsychotics. The control group receivedroutine psychiatric nursing, and the observation group received targeted rehabilitation training additionally. The rehabilitation effectwas assessed by Scale for Assessment of Negative Symptoms (SANS) and Inpatient Psychiatric Rehabilitation Outcome Scale (IPROS). ResultsThe scores of lack of interest, attention dysfunction, ability of daily life, social function, concern and interest were lower in observationgroup than in control group (P<0.05). Conclusion Rehabilitation training during hospitalization can improve the social function of schizophrenics.

Through demonstrating the satisfaction evaluation system of Singapore hospitals,the article analyzed the differences between the satisfaction evaluation system of Singapore and the system made by Shanghai Municipal Commission of Health and Family Planning.It was concluded that the humanized evaluation methods should be highlighted,the conformity of establishment of evaluation system and policy be focused on,staff incentive mechanism be focused on and perfected,and patients' expectation indicator and perceived quality be considered as strategic tools to improve the healthcare system performance.

Mesenchymal stem cells is another adult stem cells in bone marrow apart from hematopoietic stem cells.Because of their strong self-renewal capacity and the potential of multi-lineage differentiation.mesenehymal stem cells are used more and more in the treatment of malignant diseases,such as hematological malignant diseases and extensively applied in regenerative medicine and tissue engineering.However,the exact role of mesenehymal stem cells on tumor is not completely clear.This review is about the impact of mesenchymal stem cells on tumor growth and the related mechanism.

Cranial irradiation is routinely used for the treatment of almost all brain tumors,but it may cause disastrous injury in brain,especially in the hippocampus that has cognitional and emotional regulation functions.Several studies have investigated the mechanisms of radiation-induced hippocampal dysfunctions such as hippocampal neurogenesis,neurotrophin and neuroinflammation,and provided effective prevention strategies such as exercise,environmental enrichment,anti-inflammatory and intrahippocampal transplantation of neural stem cells.This review discussed the curent progresses and challenges of hippocampal dysfunction induced by cranial irradiation and provided new insights for preclinical and clinical researches.

AIM: To establish a method of determining eucalyptol, camphor, menthol and borneol in Ganmaokang Spray. METHODS: GC was conducted on a EC-WAX column(30 m?0.53 mm?1.2 ?m) and naphthalene was adopted as internal standard. RESULTS: Eucalyptol, camphor, menthol and borneol respectively showed a good linear relationship in the following ranges of concentration:0.384 48-0.987 12 mg/mL,0.240 6～(0.561 4 mg/mL,)0.367 56-0.857 6 mg/mL and 0.135 6-0.316 4 mg/mL. The average recoveries were obtained as 98.4%, 98.2%, 98.5% and 98.2%(n=5). CONCLUSION: The method is simple, sensitive, accurate and suitable for the determination of four constituents in Ganmaokang Spray.

DNA Mutational Analysis ; Drug Resistance ; genetics ; Genetic Variation ; Glucocorticoids ; pharmacology ; Humans ; Mutation ; Receptors, Glucocorticoid ; classification ; genetics ; physiology

Granulocyte macrophage-colony stimulating factor (GM-CSF) is a muhifunctional growth factor. It stimulates the proliferation, differemiation and maturity of hematopoietic progenitor cell (HPC), and transfers from bone marrow to periphery, inducing multiple cell proliferation or differentiation. In recent years, some studies have indicated that GM-CSF plays an important role in anti-apoptosis, inducing neuronal differentiation and angiogenesis, which will he a new supplement to the treatment of ischemic cerebrovascular disease. This article reviews the effects of GM-CSF in the treatment of ischemic cerebrovascular disease.

Objective To study the clinical efficacy of naloxone combined with edaravone on acute alcoholism. Methods One hundred and twenty-five patients with acute alcoholism were randomly divided into the control and treatment groups. The control group accepted conventional treatment and taking naloxone, while the treatment group accepted edaravone on the basis. Before and after treatment,the treatment recovery time,recovery respiratory rate time and symptom disappearance time were recorded. The malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), neuron-specific enolase (NSE), S-100βand vascular cell adhesion points-1 (VCAM-1) were detected. Results After treatment,the recovery time and recovery respiratory rate time of the treatment group were significantly shorter than those of the control group ( <0.05) . In the control group, S-100β was significantly improved after treatment ( <0.05) . NSE, S-100β, VCAM-1 and MDA have significantly decreased ( <0.05) after treatment in the treatment group. Compared with the control group, there were significant differences of S-100β,VCAM-1 and MDA in the treatment group before and after treatment (<0.05) . While SOD and GSH have significantly increased ( <0.05) after treatment and showed significant differences compared with the control group ( <0.05) . Conclusion Naloxone and edaravone combination therapy can improve oxidative stress and nerve damage, which help early rehabilitation in patients with acute alcoholism.

Objective To investigate the clinical study on drynaria injection for delayed healing of fracture patients.Methods 108 patients with fracture delayed union from February 2010 to February 2016 in our hospital were selected and randomly divided into the control group and the experiment group by different treatment,54 cases in each group.The control group were treated by conventional therapy,the experiment group were treated with drynaria rhizome injection on the bases of control group.The total effective rate,serum alkaline phosphatase,NGF and BMP-2 levels were observed and compared after treatment.Results After treatment,the total effective rate of experiment group was higher than control group (P<0.05);After treatment for 7 and 14 days,the serum alkaline phosphatase level of the two groups were significantly increased (P<0.05),and compared with the control group, the serum alkaline phosphatase content of the experimental group was higher (P<0.05);After treatment for 7 and 14 days,the serum NGF and BMP-2 levels of two groups were significantly increased(P<0.05),and compared with the control group,the experimental group was higher than those in the control group.Conclusion Injection of rhizoma drynariae in the treatment of delayed healing of patients with significant effect,increase the levels of serum alkaline phosphatase, serum NGF and BMP-2,promote fracture healing in patients and improve clinical efficacy.

Objective To explore relationship between the changes of mononuclear cells (MNCS) apoptosis and the levels of inflammatory cytokines and adhesion molecules in patients with systemic lupus erythematosus(SLE). Methods The apoptotic rates of MNCS in 32 patients with SLE and 10 healthy subjects were detected with flow cytometry, and the plasma levels of inflammatory cytokines(IL-8, IL-6,TNF-? and NO) and adhesion molecules(P-Sel, ICAM-1) were assayed with ELISA. Results The percentage of MNCS apoptosis in the patients with active stage of SLE was obviously higher than that in healthy subjects and patients with remission stage of SLE, and there was no significant difference in the percentage of MNCS apoptosis between healthy subjects and patients with remission stage of SLE. The levels of plasma IL-8, TNF-?, IL-6, NO, P-sel and ICAM-1 in patients with active stage of SLE were significantly higher than those in healthy subjects and the patients with remission stage of SLE, and were positively correlated with the percentage of MNCS apoptosis and the severity of SLE.There was no significant difference in the levels of plasma inflammatory cytokines and adhesion molecules between patients with remission stage of SLE and healthy subjects. Conclusion The apoptotic rate of MNCS increased in the patients with active SLE, and was closely associated with the severity and efficacy of SLE. The high expressions of inflammatory cytokines and adhesion molecules may be the reason of MNCS apoptotic increase. Appropriately regulating the expression of cytokines and adhesion molecules, and moderately controlling the apoptosis of MNCS may improve the prognosis of SLE.