Objective To explore the diagnostic value of multiplanar reconstruction(MPR)of spiral CT in the disorders of lumbar intervertebral discs.Methods Fourty-one cases with the disorders of lumbar intervertebral discs were examined with conventional CT scans and MPR of spiral CT.CT findings of intervertebral discs at L 3/4 ,L 4/5 and L 5/S 1 were compared and observed.Results The conventional scans showed bulging and /or herniation of intervertebral discs in 35 cases,and normal in 6 cases which compression of nerveroots by intervertebral discs or osseous narrow of intervertebral discs were showed on MPR.The MPR showed compression of nerve roots,compression of dural sac or osseous narrow of intervertebral foramens in 36 cases.Conclusion MPR possesses important value in diagnosing the disorders of lumbar intervertebral discs,showing the causes of lumbar vertebral diseases and distinguishing the various diseases of lumbar vertebrae.

This review summarizes the present situation of research, development and application of the Chinese herbal feed additives and its prospect. It can provide scientific references for researchers to carry out in-depth study. Chinese herbal feed additives will hopefully solve problems such as antibiotic residues, environmental pollu-tion, low productivity which hinders the development of livestock and provides strong support for developing green husbandry, meeting the food safety demand, closing the gap of livestock between the developed countries and Chi-na, increasing competitiveness of Chinese animal products in world markets. The development of Chinese herbal feed additives have very important economic and social benefits.

Objective To explore the significance of calcification in diagnosis of thyroid mass , and to improve the differential diagnosis of benign and malignant thyroid nodules .Methods 286 cases with thyroid masses admitted to the hospital from Jan .2008 to Dec.2012 underwent preoperative ultrasound scan .The clinical data were retrospectively analyzed from patients with or without calcification , the characteristics of different calci-fication and the comparison between preoperative diagnosis and postoperative pathological results .Results Ultra-sound and pathological analysis showed there was significant difference between calcification group and non -calci-fication group in detection rate of thyroid cancer (66.98%vs 13.51%, P<0.05).Detection rate of thyroid car-cinoma was significantly higher in microcalcification group than in coarse calcification group ( 84.42% vs 20.69%,P<0.05) .There was significant difference in detection rate of benign and malignant nodules in the two types of calcification group(P<0.05).The detection rate was higher in the malignant nodules than in the benign nodules .The detection rate of thyroid cancer had no statistical significance in non-calcification group and coarse calcification group ( P>0.05 ) .Conclusion Thyroid calcification , especially microcalcification has important significance in diagnosis of thyroid cancer .

Accidents, Occupational ; Adult ; Female ; Humans ; Male ; Nitrogen Mustard Compounds ; poisoning ; Occupational Exposure ; adverse effects

Hepatocyte is the core raw materials of bioartificial liver support system, primary hepatocyte is lim-ited to application because of short survival and difficult cul-ture in vitro. Porcine hepatocyte which has been used re-cently exist the risks of endogenous retrovirus transmission.With the development of molecular biology, it has been pos-sible that hepatocyte is immortalized recently. Immortalized hepatocytes have greatly significant to drug toxicology, bio-artificial liver support system and tissue engineering of liver.Therefore, we will review the prospects for research and ap-plication of immortalized hepatocytes.

Objective To investigate the resistance rate of Helicobacter pylori (Hp) to clarithromycin and its correlation with point mutations in 23S rRNA gene. Methods Hp was cultured from gastric biopsy specimen obtained from 189 patients undergoing upper gastrointestinal endoscopy. The DNA of 11 clarithromycin sensitive Hp and 19 clarithromycin resistant Hp was extracted, and 23S rRNA was amplified and sequenced. Results The rate of clarithromycin resistance in cultured Hp was 29. 2%. Point mutations in 23S rRNA gene were found in 17 clarithromycin resistant Hp strains. The proportion of A to G mutation was 36.8%, G to A of 21.5%, C to T of 15.8%, A to C of 10.5% and T to C of 5.3%. No point mutation in 23S rRNA was detected in other 2 clarithromycin resistant and 11 sensitive Hp strains. Conclusion The resistance to clarithromycin is common in Hp, and point mutations in 23S rRNA gene of Hp are frequent in clarithromycin resistant strains, with most prevalent mutations of A to G and next G to A.

Objective To explore relationship between the changes of mononuclear cells (MNCS) apoptosis and the levels of inflammatory cytokines and adhesion molecules in patients with systemic lupus erythematosus(SLE). Methods The apoptotic rates of MNCS in 32 patients with SLE and 10 healthy subjects were detected with flow cytometry, and the plasma levels of inflammatory cytokines(IL-8, IL-6,TNF-? and NO) and adhesion molecules(P-Sel, ICAM-1) were assayed with ELISA. Results The percentage of MNCS apoptosis in the patients with active stage of SLE was obviously higher than that in healthy subjects and patients with remission stage of SLE, and there was no significant difference in the percentage of MNCS apoptosis between healthy subjects and patients with remission stage of SLE. The levels of plasma IL-8, TNF-?, IL-6, NO, P-sel and ICAM-1 in patients with active stage of SLE were significantly higher than those in healthy subjects and the patients with remission stage of SLE, and were positively correlated with the percentage of MNCS apoptosis and the severity of SLE.There was no significant difference in the levels of plasma inflammatory cytokines and adhesion molecules between patients with remission stage of SLE and healthy subjects. Conclusion The apoptotic rate of MNCS increased in the patients with active SLE, and was closely associated with the severity and efficacy of SLE. The high expressions of inflammatory cytokines and adhesion molecules may be the reason of MNCS apoptotic increase. Appropriately regulating the expression of cytokines and adhesion molecules, and moderately controlling the apoptosis of MNCS may improve the prognosis of SLE.

Objective To investigate the effect of emulsified isoflurane preconditioning on postsynaptic density protein 95 (PSD95) activation in brain during focal cerebral ischemia-reperfusion (I/R)injury in rats.Methods Thirty-two pathogen-free male SD rats weighing 280-300 g were randomly divided into 4 groups (n =8each): sham operation group(group S),group I/R,emulsified isoflurane group(group EI) and lipid emulsion group(group LE).Focal cerebral I/R was induced by middle cerebral artery occlusion for 2 h followed by reperfusion.8％ emulsified isoflurane 10.5 ml/mg or 30％ lipid emulsion 10.5 ml/mg was injected intraperitoneally at 24h before I/R in groups EI and LE respectively.The neurologic deficit score (NDS) was evaluated at 6 h of reperfusion and then 4 rats were sacrificed,and brains were removed for determination of phosphorylatied PSD95 (pPSD95) expression in ischemia cortex and hippocampus by Western blot.Four rats were sacrificed at 24 h of reperfusion and then their brains were removed for determination of infarct volume percentage.Results NDS,infarct volume percentage and pPSD95 expression in ischemia cortex and hippocampus were higher in groups I/R,EI and LE than in group S( P ＜ 0.01 ).NDS,infarct volume percentage and pPSD95 expression in ischemia cortex and hippocampus were lower in group EI than in group I/R( P ＜ 0.05).There was no significant difference in NDS,infarct volume percentage and pPSD95 expression between groups I/R and LE(P ＞ 0.05).Conclusion Emulsified isoflurane preconditioning can attenuate focal cerebral I/R injury in rats by inhibiting PSD95 activation in brain.

ObjectiveTo investigate the effects of emulsified isoflurane preconditioning on the expression of platelet-activating factor (PAF) and PAF receptor during focal cerebral ischemia-reperfusion (I/R) in rats.MethodsThirty-two healthy adult male SD rats weighing 250-300 g were randomly divided into 4 groups ( n =8each):group sham operation (group S); group I/R; group emulsified isoflurane preconditioning( group EI) and group lipid emulsion (group LE).Focal cerebral I/R was induced by 2 h occlusion of middle cerebral artery followed by reperfusion in groups I/R,EI and LE.8％ emulsified isoflurane 10.5 ml/kg and 30％ lipid emulsion 10.5 ml/kg were injected intraperitoneally at 24 h before cerebral ischemia in groups EI and LE respectively.The neurologic deficit score (NDS) (0 =no deficit,4 =unable to control) was evaluated at 12 h of reperfusion.Venous blood samples were collected for measurement of plasma PAF concentration.The animals were then sacrificed and their brains removed for determination of infarct size (by TTC staining) and PAF receptor expression in hippocampus and cerebral cortex (by Western blot).ResultsFocal cerebral I/R significantly increased NDS,the infarct size,plasma PAF concentration and PAF receptor expression in cerebral cortex and hippocampus in group I/R as compared with group S.Emulsified isoflurane preconditioning significantly attenuated the focal cerebral I/R induced above changes in group EI as compared with group I/R,but there was no significant difference between groups I/R and LE.ConclusionEmulsified isoflurane preconditioning can attenuate focal cerebral I/R injury by inhibiting PAF and PAF receptor expression.

Cells and scaffolds are two key elements of tissue engineering,while the viabilitv of cells is mainly depended on the nature of scaffolds.A suitable scaffold should simulate the internal environment as well as possible,which is necessary for the growth of cells.The nanometer structured biomaterials have become more and more popular in the study of tissue engineering due to their ability to perfectly simulate the topography of the microenvironment.This paper summarized the advances in the application of nanometer structured materials in the in vitro cell cultures.The mechanisms of the promoting effect of nano.structured biomaterials on the cell growth and the application of nano-structured materials in liver tissue engineering are discussed.