Acupuncture Therapy ; Aged ; Female ; Humans ; Middle Aged ; Olfaction Disorders ; etiology ; physiopathology ; therapy ; Sinusitis ; complications ; Smell

Congresses as Topic ; Humans ; Milk Banks ; Milk, Human ; North America

Dizziness ; classification ; epidemiology ; Humans ; Vertigo ; classification ; epidemiology

Objective:To discuss a simple method for flow cytometric analysis of cell DNA stained with DAPI and Hoechst33342.Methods:HT 29 cells stained with DAPI,Hoechst33342 or PI were measured by BD FACSAria and the percentages of cells in G0/G1,S and G2/M phases with three staining me-thods,then the results were analyzed and compared.Before measurement we monitored the quality of DNA analysis of flow cytometer through UV beads QC experiment and analyzed the standard chicken erythrocyte nuclei(CEN) and calf thymocyte nuclei(CTN) stained with DAPI and Hoechst33342.Results:CV value of UV peak was 2.4 after QC experiments.There were 4 peaks on CEN histograms and the ratios of peak channel mean of G2/G1,G3/G1,and G4/G1 were about 2,3,and 4 respectively.Both CV values of the first peak were 2.4.There were 2 peaks on CTN histograms and the ratio of peak channel mean of G2/G1 was 1.97,and CV value of G0/G1 2.4.The complete cell cycle of HT29 cells stained with DAPI,Hoechst33342 or PI was showed entirely,CV values were 3.40,3.02 and 4.42,respectively,and the percentages of cells in G0/G1 were 60.86%,60.22% and 60.81%,respectively,in S,28.85%,29.70% and 29.82%,respectively,and in G2/M,10.29%,9.09% and 9.37%,respectively.The results by the three methods showed no difference.Conclusion:This method for measurement of cellular DNA content is a simple and efficient approach to determining cell cycle and can be the first choice when using flow cytometer with 355 nm UV.

Objective To explore the relationship between metabolic syndrome(MS) and cerebral stroke type,complication and prognosis.Methods Clinical data of 568 cerebral stroke patients with MS(MS group) and 209 cerebral stroke patients without MS(no-MS group) were compared and analyzed.Results In MS group,the incidence of cerebral infarction was significantly higher(93.0%) than that in the no-MS group(85.2%)(P

Airway obstruction in children is a rare,in recent years,with the development of CT technology and the development of fiber bronchoscopy,the number of cases of children with tracheal stenosis has increased year by year,but difficult clinical problem,with no clear agreement on optimal therapeutic approach.Stenting of the airway has been used successfully in adults,and is an attractive alternative in children.Fundamental differences of pediatric compared to adult use include the benign nature of most stenoses,the narrow and soft airways of children,the required long-term tolerance and adaptation to growth.So it is controversial in the indications.

Objective To understand the protective effects of children after hepatitis B vaccine immunization since 1992,when the hepatitis B vaccine into childhood immunization strategy .Methods Hospitalized children from venous blood,using enzyme-linked immunosorbent assay(ELISA),to investigate and analyze the immune status based hepatitis B immune of hospitalized children (Infection,Pediatric),by five indicators of hepatitis B as the judgment standard.Results The total anti-HBs-positive rate was 55.05%,including infection anti-HBs-positive rate 52.18%, the pediatric anti-HBs-positive rate 55.92%.Male anti-HBs positive rate was 54.17%,the female anti-HBs positive rate was 56.6%,there was no statistical significance among departments and sexs (P>0.05).Conclusion Hepatitis B vaccination is the effective control means for hepatitis B ,however,the anti-HBs positive rate is low in this region after hepatitis B vaccine immunization ,needed to reseed .

Monitoring data of antibiotic susceptibility from both home and abroad showed that carbapenem-resistant Enterobacteriaceae (CRE) are being on the rise,which represents an increasingly urgent clinical danger and an emerging public health concern worldwide.In order to effectively prevent CRE transmission in healthcare settings,we should not only pay high attention to the capability building of the laboratory of clinical microbiology,but also actively explore into the mechanisms of carbapenem resistance in Enterobacteriaceae.What's more,we should substantially raise the detection capacity of laboratory on CRE,effectively carry out the antimicrobial stewardship program,and strictly implement hand hygiene and contact precautions.(Chin J Lab Med,2013,36:300-302)

Objective To realize the current status of central sterile supply departments(CSSDs)in hospitals,and provide the basis for making improvement measures.Methods Field investigation was adopted to study the resource distribution,personnel structure,and the quality of medical instrument cleaning in 78 hospitals in Chongqing.Re-sults Qualified results of 78 hospitals was 24.36% (19/78),basically qualified rate 52.56% (41/78),unqualified rate 23.08% (18/78);inspection results of different types of hospitals were significantly different(Hc= 16.643,P=0.002),qualified hospitals mainly concentrated on city- and county-level hospitals.Unqualified rate of CSSD con-struction area was 78.21% (n= 61),unqualified rate of personal allocation was 75.64% (n= 59),concentrated man-agement of operating rooms and CSSDs was 52.56% (n= 41),mainly concentrated on city- and county-level hospi-tals;decentralized management accounted for 47.44% (n= 37),58(74.36% )hospitals used repeated cleaning stain-less steel tables for receiving,50% didn’t install automated cleaning machine and drying cabinet,42.31% (33/78) performed manual cleaning,40(51.28% )didn’t equip with water treatment system;12(15.38% )hospitals used patched or worn clothing for packing,47 didn’t equip with hard metal container,37(47.44% )had no heat sealing machine,21(26.92% )had no detection equipment for cleaning quality,41(52.56% )had no low temperature sterili-zation instruments;52(66.67% )hospitals performed biological monitoring on high pressure steam sterilizer.Conclu-sion Clean and disinfection equipments in CSSDs in city- and county-level hospitals are well-appointed,procedures of clean and disinfection are standard,and can achieve the standard of clean quality;while hospitals affiliated to fac-tories and private hospitals are not well-appointed,procedures of clean and disinfection are not standard. Construc-tion of CSSD should be standard,so as to ensure the effectiveness of clean,disinfection and sterilization of surgical instruments.

In the treatment of human epidermal growth factor receptor 2 (Her-2) overexpressing breast cancer,some patients have drug resistance to trastuzumab.Potential mechanisms of resistance to trastuzumab include impaired access of trastuzumab to Her-2 ; alternative signaling from insulin-like growth factor-1 receptor (IGF-1R),hepatocyte growth factor receptor (HGFR) and so on; aberrant downstream signaling caused by loss of phosphatase and tensin homologs deleted from chromosome 10 (PTEN);phosphatidylinositol 3-kinase catalytic alpha polypeptide gene (PIK3CA) mutation; over-expression of heat shock protein 90 (HSP90) and CD44. Additionally,potential strategies for overcoming resistance to trastuzumab include using new targeted medicines,such as pertuzumab,lapatinib and trastuzumab-DM1.