Objective To analyze the relative factors of bone marrow suppression after chemotherapy with different-dose carboplatin and paclitaxel (TC) on the patients with ovarian cancer.Methods Sixty-two patients with ovarian cancer admitted in Beijing Obstetrics and Gynecology Hospital from January 2002 to December 2007, using TC regimen ,a total of 196 cycles of chemotherapy ,were divided into two groups by the doses of carboplatin [area under concentration-time curve(AUC) 4 -6 for low-dose,AUC >6 -7 for hight-dose, the carboplatin dose calculated with AUC] or by the doses of paclitaxel (135 -< 150 mg/m2 low-dose,150 - 175 mg/m2 hight-dose). After each TC cycle, the routine blood was test to determine the graduation of the marrow suppression, and then the correlation factors were analyzed with logistic regression. Results (1) The occurrence rate of bone marrow suppression:there were 159 cycles (81.1%) grade 0 - Ⅱ bone marrow suppression, while 37 cycles (18.9%) of grade Ⅲ - Ⅳ. (2) Factors related to bone marrow suppression:the results shown that there were not related to bone marrow suppression,which incluced cellular differentiation, tumor type, height, weight and paclitaxel dose(P>0.05). While,the different cycle, age, the later stages of tumor, serum creatinine concentration, endogenous creatinine clearance rate, AUC values were the relative factors of bone marrow suppression(P =0.000,0.000,0.018,0.033,0.001,0.000). Seven variables were conducted into the logistic regression and the results shown that the different cycles, the age, AUC grades were independent risk factors (P = 0.030,0.043,0.009).(3) When low-dose of paclitaxel was given, the occurrence of bone marrow suppression was related to the carboplatin dose AUC. The higher AUC values for carbopaltin were chosen, the higher of severe bone marrow suppression would happen. (4/14 vs 0,P=0.015). When the dose of high grade of paclitaxel was given, the occurrence of bone marrow suppression in cases with hight-dose carboplatin was statistically significant than that in cases treated with low-dose carboplatin [45.7%(16/35) vs 13.7% (17/124), P=0.000]. Conclusions The independent risk factors of myelosuppression after chemotherapy with TC regime on the patients with ovarian cancer including the cycles, age and AUC values. The carboplatin dose calculating with AUC is related to the occurrence of bone marrow suppression, the higher AUC values for carbopaltin would chosen,the higher of severe bone marrow suppression would be happen.

Objective To observe the efficacy and side effect of olanzapine combined with tropisetron, dexamethasone for the pre-vention of highly emetogenic chemotherapy-induced nausea and vomiting ( CINV) . Methods A total of 78 patients with highly emetogen-ic single day chemotherapy were randomly divided into two groups:trial group ( olanzapine combined with tropisetron, dexamethasone n=40) and control group (Tropisetron Combined with Dexamethasone n=38). The control of acute CINV, delayed CINV and adverse reac-tions were observed. Results The control rates of acute vomiting in trial group and control group were 87. 5% vs 81. 6%, 65. 0% vs 57. 9% in acute nausea, 75. 0% vs 52. 6% in delayed vomiting, 32. 5% vs 13. 2% in delayed nausea. The trial group was better than the control group in delayed vomiting and delayed nausea which there was difference between them (P < 0. 05). There was no statistical differ-ence between the two groups in the incidence of adverse reactions (P>0. 05). Conclusion Olanzapine combined with tropisetron, dexa-methasone for the prevention was significantly better than tropisetron combined with dexamethasone in the control of delayed CINV in pa-tients received highly emetogenic chemotherapy.

ObjectiveTo explore the ability of inhibition resistibility of multiple displacement amplification (MDA)in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods(MBM)to purify sample.MethodsDifferent concentra-tions of hemoglobin and humid acid(HA)mixed with DNA samples and then divided the samples into MDA group, MBM group and control group.D3S1358locus was amplified and detected by polyacry-lamide gel electrophoresis detection system and AmpF?STR? IdentifilerTM Plus Kit-capillary electrophore-sis detection system.ResultsWhen hemoglobin concentrations exceed 1 ng/μL or HA concentrations ex-ceed 0.1 ng/μL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/μL or HA concentrations exceed 1 ng/μL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors.ConclusionMDA has the capability to remove the inhibi-tion of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.

Objective:To analyze the application effect of a new type of intelligent and portable nursing device for reducing risk in nursing of operating room so as to wholly enhance the nursing quality for risk in nursing of operating room and ensure the nursing safety of operating room in hospital.Methods: 200 patients treated with abdominal surgery were divided into observation group (100cases) and control group (100cases) as the different hospital admission time. The patients of control group received routine nursing measurement, while patients of control group received new type of intelligent and portable nursing device on the basic routine nursing of observation group. The nursing quality and degree of satisfaction of patients for nursing measurement were compared and analyzed.Results: The satisfaction of patients for nursing measurement of observation group was significantly higher than that of control group, and the differences of professional skill in nursing, the interpretation for state of disease and service attitude between the two groups were statistically significant (t=10.366,t=15.350,t=12.671,P<0.05). And the differences of nursing quality score including basic nurse, sterile situation and safety management between the two groups were statistically significant (t=8.6134,t=6.0156,t=3.3048,P<0.05).Conclusion: This utility model nursing device of operative room adds shelf of infusion based on the traditional electrocardiograph monitor, and it is convenient in installation, disassembly, and transportation and carrying. At the same time, it can reduce the covering an area of space and monitor the process of infusion in time so as to remind medical staffs to change fluid. Therefore, it is convenient for medical staffs and is worthy in clinical practice.

Objective To compare the clinical efficacy of low incision thyroidectomy and traditional thyroid surgery in the treatment of thyroid nodules.Methods 84 patients with thyroid nodules were included in the study.According to the order of treatment,42 cases who were compiled as single number were selected as the control group,and they were given traditional thyroid surgical treatment.While 42 cases who were compiled as double number were selected as the observation group,and they were given low incision thyroidectomy.The clinical effects were compared between the two groups.Results The incision length[(3.72±0.59)cm],the intraoperative blood loss[(41.68±3.57)mL],the operation time[(38.27±4.16)min],the hospitalization time[(4.32±1.07)d]in the observation group had statistically significant differences compared with the control group(t=6.886,7.429,7.280,6.775,all P<0.05).The total effective rate in the observation group was 92.86%,which was higher than 73.81% in the control group,the difference was statistically significant(χ2=7.445,P<0.05).The incidence rate of postoperative complications in the observation group was 7.14%,which was lower than 26.19% in the control group,the difference was statistically significant(χ2=8.564,P<0.05).Conclusion Compared with traditional thyroid surgery,the low incision thyroidectomy is effective in the treatment of thyroid nodules,which can reduce the amount of intraoperative blood loss,shorten the operation time and hospitalization time,and has less trauma,quick recovery and low incidence of complication,and it can be used as the first choice for clinical treatment.

Objective To analyze the application of antimicrobial agents before and after the improvement of prescription evaluation system in Green Town Cardiovascular Hospital.Methods One thousand outpatient prescriptions of antimicrobial agents were randomly selected from our hospital from January 2014 to January 2016,serving as the data before intervention.These prescriptions were analyzed according to the prescription evaluation system and effective interventions were proposed according to the existing problems in prescriptions.Another 1 000 effective prescriptions of antimicrobial agents during February 2015 to January 2016 in our hospital were taken as the data after intervention.Unreasonable use of antimicrobial agents and the satisfaction degree of physicians,nurses and patients towards the prescription were compared before and after intervention.Results After intervention,the percentage of unreasonable use of antimicrobial agents from May 2015 to May 2016 (2.10%) was significantly lower than that from April 2014 to April 2015 (30.00%),the difference was statistically significant (χ2=288.857,P<0.05).The satisfaction degree of physicians,nurses and patients towards the prescription from May 2015 to May 2016 [(93.23±5.93)points,(95.29±6.01)points,(68.99±4.27)points] was significantly higher than that from April 2014 to April 2015 [(75.34±5.32)points,(71.46±5.13)points,(68.99±4.27)points],the difference was statistically significant (t=110.706,95.368,123.711,all P<0.05).Conclusion The improvement of prescription evaluation system can obviously improve unreasonable use of antimicrobial agents,increase the satisfaction degree,avoid drug abuse,produce obvious effect and great significance.

OBJECTIVE To investigate the effectof Myrtol combined with Cefazolin Sodium Pentahydrate in the treatment of acute suppurative otitis media in children. METHODS Totally 120 cases of pediatric acute suppurative otitis media were selected in our hospital from January 2014 to January 2016 and randomly divided into four groups, treated with Myrtol combined with Cefazolin Sodium Pentahydrate, Myrtol combined with Cefazolin Sodium, Cefazolin Sodium Pentahydrate, or Cefazolin Sodium, respectively. The effect and adverse reactions of the 4 groups were studied and compared. RESULTS There was no significant differences in age, sex, and average course of disease among the four groups. In Myrtol combined with Cefazolin Sodium Pentahydrate group, the clinical efficacy is better than that of the other three groups (P<0.05).There was no difference between Myrtol combined with Cefazolin Sodium group and the Cefazolin Sodium Pentahydrate group(P>0.05), and both of them are better than cefazolin sodium group(P<0.05). For the disappearance time of symptoms, including earache, hearing loss, fever and tympanic hyperaemia, Myrtol combined with Cefazolin Sodium Pentahydrate treatment was shorter than the other three groups (P<0.05). There was no significant differences among the three groups(P>0.05). CONCLUSION Myrtol combined with Cefazolin Pentahydrate Sodium treatment was superior to the other three protocols in clinical efficacy for children with acute suppurative otitis media , and does do not increase the risk of safety.

Cre/LoxP site specific recombinase system is an effective tool for gene recombination in the body to produce specific gene deletion or inversion.The study of gene function is the experimental basis for the development of experimental animal models.Study the Cre/LoxP system,compare the different function characteristics of mouse models for Cre recombinase system in the research of ophthalmology,epecially in the tissue of retina,such as retinal pigment epithelium (RPE) cells,Müller cells and photoreceptor cells,get the Cre transgenic mouse model to establish proper experimental animal models of ophthalmology will lay a solid foundation for the research of the lack of single gene for a particular organization study.Here,the research progress of Cre/LoxP system,the Cre transgenic mouse models and eye specific Cre recombinant enzyme model mice will be summarized.

Objective To investigate the effect of implementing the triage standard in pediatric emergency to provide a basis for the nursing triage system.Methods The self-designed questionnaire was adopted to investigate the satisfaction degree on the triage nursing work from patients,emergency nurses,triage nurses and doctors.Results The satisfaction degrees of the patients,doctors and nurses on the triage nursing work after implementation of the triage system were significantly increased compared with before implementation(P<0.05).Conclusion Implementing the pediatric emergency triage system can effectively identify critical chil-dren,make them to obtain timely treatment and conduce to increase the triage quality.

Objective To compare efficacy and safety of clavicular hook plate and Kirschner wire in treat-ment of clavicular fracture.Methods 72 patients with clavicle closed fracture were chosen as the research objects, they were randomly divided into the observation group and control group according to randomly number table,the con-trol group were treated with Kirschner wire intramedullary fixation,the observation group were treated with clavicular plate S plate internal fixation.The effect and the complication rate of two groups were compared.Results In the observation group,the fracture healing time was (13.51 ±1.06) weeks,the control group was (18.35 ±2.14) weeks, there were significant difference between the two groups in fracture healing time( t=5.54,P<0.05);postoperative follow-up in the observation group,the excellent and good rate was 94.44%,69.45%in the control group,the excel-lent and good rate of two groups had significant difference(χ2 =4.16,P<0.05);the observation group the postopera-tive complication rate was 47.22%,the control group was 77.78%,complications had significantly different(χ2 =5.21,P<0.05).Conclusion Compared with Kirschner wire,,S plate internal fixation for treatment of clavicle closed comminuted fracture has effective in treatment and high safety,which is worthy of further clinical application.