Animals ; Caspase 1 ; genetics ; metabolism ; Disease Models, Animal ; Female ; Flurothyl ; Gene Expression Regulation ; Hippocampus ; metabolism ; pathology ; Interleukin-18 ; genetics ; metabolism ; Interleukin-1beta ; genetics ; metabolism ; Male ; RNA, Messenger ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Seizures ; metabolism ; pathology ; Time Factors ; Up-Regulation

Objective To observe the clinical effectiveness and prognostic factors of autologous hemopoietie stem cell transplantation on diabetic peripheral angiopathy patients under different degrees of vascular lesions. Methods Thirty-six patients with diabetic were divided into mild vascular stenosis group (group A: 20 lower extremities, vascular stenosis less than 50% ), moderate vascular stenosis group(group B :26 lower extremities, vascular stenosis of 50%-70% ) and severe vascular stenosis group (group C: 25 lower extremities, vascular stenosis greater than 70% ) according to the degree of vascular lesions. All patients were given the stem cell mobilization for 5 days, then peripheral hemopoietic stem cells were gathered on the last day and transplanted to ischemic limb by using monoeyte stem cell suspend liquid with 3 cm × 3 cm intervals.Each index of three groups was regularly observed ( 1th,12th and 18th month respectively after the operation)and comprehensive evaluated. Meanwhile,the transplantation prognostic factors (age, disease duration, post-prandial 2 hours blood glucose, glycated hemoglobin, blood pressure, triglycerides) were analyzed by multiple regression. Results Twelve months after transplantation, the ankle brachial index (ABI) and percutaneous oxygen pressure (TcPO2) of all three groups increased compared with that before the transplantation (P<0.05). In the 18th month after transplantation, the ABI still increased in group A and B (P< 0.05), and no significant changes were found in group C compared with that in the 12th month. Eighteenth month after transplantation, lower extremities of 8 patients (40%) in group A, 8 patients (31%) in group B and 6 patients (24%) in group C were examined to have neovascularizatian. Age, disease duration, blood pressure, post-prandial 2 hours blood glucose, glycated hemoglobin and triglycerides were all the important factors that affected diabetic peripheral angiopathy recurrence after transplantation. Conclusions The long-term effica-cy of stem cell transplantation on diabetic peripheral angiopathy patients at a mild to moderate degree is superior to that with severe vascular lesions. Close monitoring of blood glucose, blood pressure and triglycerides in the operation perinatal stage is required.They are the important prognostic indicators.

砄bjective:To fabricate bone tissue that has similar structural and mechanical characters with normal bone.Methods: Titanium meshes were molded into the shape of column in the length of 12 mm and in the diameter of 8 mm. The column was filled with natural coral granduls.4?10 7 marrow derived osteoblasts in 200 ?l cell culture medium were seeded into each of five scaffolds and incubated in vitro for 2 d to ensure that cells adhere well on the scaffolds. Then the scaffolds were implanted subcutaneously into the back of nude mice. Two months after implantation, the animals were sacrificed and the implanted materials were investigated by gross specimen inspection, X ray examination and histological observation. Results:2 months after in vivo incubation, the newly formed tissue was red and had the gross appearance of bone, and kept the original shape of column. Titanium mesh situated in the surface area. X ray examination showed that large amount of new bone formed in the scaffolds, there was no space between new bone and titanium mesh. Most of coral granduls had been absorbed. Histological observation demonstrated that in the surface area, new bone integrated well with titanium mesh and was enforced by titanium mesh(like cortical bone), and in the middle area large amount of lamellar bone formed.Conclusion: Newly formed bone in this experiment has similar structural with normal cortical bone.

Gastric cancer has a high incidence and accounts for a notable proportion of global cancer mortality.The pathogenesis of gastric cancer is complex involving multiple genetic and epige-netic alterations factors.In recent years,studies have also dem-onstrated the aberrant regulation of miRNAs in a variety of tumor pathological processes including the gastric cancer through af-fecting cell proliferation,differentiation and apoptosis.miRNA also plays important roles in gastric cancer growing,invasion, metastasis,and drug resistance of gastric cancers,which might become new biomarkers and potential therapeutic targets in the future.This paper summarizes several important miRNAs in-volved in gastric cancer and also discusses their function.

Objective To construct and express fusion toxin human IL15 mutant/TNF? mutant (hIL15M/TNF?M) in order to develop a targeted toxin capable of eliminating abnormal cells expressing high levels of hIL15 receptors. Methods DNA fragments of truncated hIL15M and TNF?M coding gene were joined in frame and cloned into T7 expression vector pET16b. The expressed fusion protein was purified from E. coli . Cytotoxicity of hIL15M/TNF?M was measured by MTT assay. Results E. coli transformed with the recombinant plasmid expressed an extra band with the expected size of 30?10 3. MTT assay indicated that PHA activated T cells were 13 times more sensitive to the cytotoxic effect of hIL15M/TNF?M than to the resting T cells. Conclusion hIL15M/TNF?M has high toxic effect against cells expressing high levels of IL15 receptors, suggesting that hIL15M/TNF?M has potential therapeutic effect on T cell leukemia and other diseases related with improperly activated T cells.

OBJECTIVE:To improve medication safety and efficacy.METHODS:5different tablets broken off with hands were investigated with respect to weight variations and microbial limits by reference to Chinese Pharmacopeia.RESULTS:The5kinds of tablets broken off with hands were not in conformity with Chinese Pharmacopeia with respect to weight variation and which involved different degrees of microbial contaminations.CONCLUSION:It is recommended that pharmaceutical enter?prises should add more dosage forms of standard drugs that suitable for the use of children,women,the aged and particular patients.If the separately packaged drugs must be broken off with hands,aseptic method should be applied in which the drugs were broken off by the pharmacists on the sterile air bench before being separately packed with sterilized plastic pouches(which were sterilized with anprolene),and which should be administered to the patients for taking during the same day.

Genetic Predisposition to Disease ; Humans ; Neoplasms ; genetics ; Polymorphism, Single Nucleotide ; RNA, Long Noncoding ; genetics

Objective We aimed to investigate the role of McMaster model of family therapy in improving family function of patients with advanced hepatocellular carcinoma (HCC).Methods Patients who had advanced HCC and received transarterial embolization (TAE) or transarterial chemoembolization (TACE) from Department of Hepatobiliary Ⅰ,Eastern Hepatobiliary Surgery Hospital between October 1,2012 and June 25,2013 were randomly divided into two groups:the experimental group (51 patients) and the control group (49 patients).The control group received routine family support education.The experimental group not only executed routine family support education,but also was given McMaster model of family therapy according to evaluation results of family assessment device (FAD) on the second and third day of hospitalization.The status of family function of all patients were assessed by FAD on the fnrst day of hospitalization and fourth week after therapy.We compared the status of family function between the two groups.Results In age,gender,educational level,place of residence,occupation,family economic status,medical payment,liver or kidney function,HBV infection,cirrhosis and tumor burden,no statistical differences were found between the experimental group and the control group patients before TAE or TACE.Two groups were dysfunction in communication,roles,affective responsiveness,affective involvement,behavior control,and general function in addition to problem solving before TAE or TACE.No statistical differences were found between two groups.After the therapy,compared with the control group,those patients in the experimental group had a lower level scoring in communication,roles,affective responsiveness,affective involvement,behavior control,and general function in addition to problem solving on the fourth week after the therapy,t value was-2.544,-3.767,-3.904,-2.848,-4.950 and-4.953,the difference had statistical significance.Conclusions McMaster model of family therapy may help to improve family function of patients with advanced HCC.

Objective To understand current status of occupational protection awareness and behavior among dif-ferent professional medical interns,and provide basis for strengthening the education about occupational protection among medical interns .Methods Questionnaire survey was used to survey medical interns at the end of their clini-cal internship.Results A total of 385 cases of occupational exposure occurred during internship period,occupation-al exposure rate was 42.31 % (385/910),occupational exposure mainly occurred in nursing interns (n =190).358 (39.34%)medical interns understood self-protective antibody (hepatitis B surface antibody);difference in occupa-tional exposure rates and awareness of self-protective antibody among different professional medical interns was sta-tistically significant (all P <0.05);222 (57.66%)medical interns sustained occupational exposure more than once, 45.45% of occupational exposure occurred during the process of diagnostic and therapeutic procedure,exposure mainly occurred in the wards (32.21 %).After occupational exposure,46.75% of medical interns felt nervous, 35.06% were worried,18.18% appeared serious psychological reaction,only 14.03% sought support and help from others.Conclusion Awareness of occupational protection among medical interns is insufficient,education and awareness of occupational protection should be intensified,psychological behavioral after occupational exposure should be paid attention among medical students.