Objective To analyze the current research focus of solid organ transplantation in order to provide information for Chinese clinicians and researchers for research direction and topic selection.Method By using bibliometric method,the Chinese Science Citation Database and Web of Science were thoroughly searched and highly cited papers concerning solid organ transplantation were summarized and analyzed from 2010 to 2013.With the year of publication,national and institutional distribution,source journals,column type,research content as indicators,all the keywords of high-frequency were analyzed via co-word analysis method in order to summarize the characteristics of hot research subject in the field of solid organ transplantation.Result From 2010 to 2013,top100 highly cited articles in Chinese had a preference for liver transplantation,followed by kidney transplantation,immunosuppressant,organ donation,postoperative complication and rejection,while articles in English mainly focused on surgical technique,recipient survival,transplant immunity and rejection.Top 100 highly cited articles were from 50 and 58 institutions for Chinese and English,respectively.There were 47 papers from top 10 institutions in Chinese,accounting for 45.6％ of the total citations.There were 43 papers from top 10 institutions in English,accounting for 47.4％ of the total citations.Top 100 highly cited papers in Chinese were distributed in 27 journals.The top 10 journals published 75 papers,which have been cited for 316 times.Top 100 highly cited papers in English were distributed in 36 journals.The top 10 journals published 70 papers,which have been cited for 7 694 times.Conclusion Domestic experts and scholars should pay continuous attention to the organ donation issues related to research in the future,with reference to the advanced experience of Europe and the United States and other countries.Solid organ transplantation registry,data center report and update,transplant recipient prognosis and long-term survival should be highly focused.

AIM: To establish an animal model of experimental autoimmune myocarditis (EAM) in BALB/c mice and to investigate the expression and significance of Toll-like receptor 3 in mouse EAM. METHODS: BALB/c mice were immunized with cardiac myosin extracted from porcine ventricular myocardium covered by complete freund's adjuvant (CFA) on 0 d and 7 d, then divided into immunized with CFA only. Serum and myocardium samples were collected at 14 d and 21 d after the first immunization. HE staining was used to identify the areas of inflammation. The myosin IgG antibody was examined by indirect ELISA assay. The changes of TLR3 protein and mRNA expression in myocardial tissue were measured by immunohistochemistry and real time-PCR. RESULTS: Compared to control group, immunohistochemistry results showed that there was positive expression of TLR3 in the myocardium of mice with EAM and the mRNA of TLR3 were more than 20 times (P<0.05). The expression of interferon beta mRNA in EAM group was more than 14 times as many as basal expression, that of tumor necrosis factor alpha was more than 18 times (P<0.05). CONCLUSION: The expression of Toll-like receptor 3 in myocardium is up-regulated in experimental autoimmune myocarditis. The inflammatory response to cardiac myosin may associate with the TLR3 signal transduction pathway.