Objective To compare the measured IOP values by non-contact tonometer and indentation tonometer in order to evaluate the non-contact tonometer in clinical application. Methods 320 eyes of 160 patients for screening of IOP in the clinic were included in the observation, by self-control study, the IOP value of the non-contact tonometer(abbreviated NCT) and the indentation tonometer was compared. Results IOP values measured by NCT was lower than that mesgured by indentation tonometer. Conclusions IOP values measured by NCT was lower than that measured by indentation tonometer, for IOP value around 2.67 kPa measured by NCT, it is best to use indentation tonometer to retest or coming again for subsequent visit.