Twenty Rota virus strains were isolated from 20 stool specimens derived from diarrhea children in Khanh Hoa province. After 3 times of passaged culturations on MAI04 cells, there were only 2 human Rota virus strains growing well in the medium of trypsin concentration at 5 mg/ml and 4 human Rotavirus strains multiflying well in the medium of trypsin concentration at 10 mg/ml.
Rotavirus ; Diarrhea, Child ; Child

Chronic diarrhea is defined as passing watery stools that lasts for more than 2 weeks. Persistent diarrhea belongs to chronic diarrhea and is a chronic episode of diarrhea of infectious etiology. The etiology of chronic diarrhea is varied. It is important to consider the child's age and clinical manifestations with alarm signals for an application of proper treatments to children with chronic diarrhea. Vicious cycle is present in chronic diarrhea and nutritional rehabilitation can break the vicious cycle of chronic diarrhea and is one of the main one thing among treatments. We should know the exact concept of chronic diarrhea and provide appropriate treatments according to etiologies of chronic diarrhea.
Child ; Diarrhea ; Humans

E. coli is an emerging diarrheagenic pathogen. Serotyping is important to identify bacteria and trace their epidemiology. Objectives: (1) To identify the distribution of serogroup O among 4 kinds of diarrheagenic E. coli strains isolated from children with and without diarrhea in Hanoi, Vietnam. (2)To find out the relationship between serogroup O and different diarrheagenic E. coli. Method: One hundred and sixty-two strains of diarrheagenic E. coli including 86 EAEC, 50 EPEC, 12 EIEC, and 14 ETEC have been serotyped by slide agglutination with antisera specific for O serogroups. Results: More than 60% of the strains were serotyped. Serogroup O25 was the most common with the prevalence of 16.7% followed by O15 (4.3%). The other serogroups (O127a, O128, O126, O6, and O16) were seen in very few numbers. 38.3% of E. coli strains were not serotypeable with available antisera. EAEC were the most prevalent among diarrheagenic E. coli and 25.6% of them belong to the serogroup O25. Conclusion: 60% of the diarrheagenic E. coli strains were serotyped. Serogroup 025 was the most common. The findings were very useful for assessing the distribution of serogroups of diarrheagenic E. coli and will be useful for future vaccine development.
Diarrhea, Escherichia coli, Child

Over 5 years we have conducted sentinel surveillance for rotavirus at six hospitals in Vietnam. More than 10,000 children less than 5 years old were admitted with diarrhea with fecal specimens screened for rotavirus. Overall, 55% were positive and there is variability in rates of rotavirus detection between sites (44%-62%). The older age children become infected, the higher percentage of rotavirus detected. the rotavirus strains appear to be the common type.
Diarrhea ; Rotavirus ; Child

The study was performed on 10.585 household including 13.422 under 5 year old age children with 9.69% acquired acute diarrhoea and a mortality of 18.18% of cases. Risk factors ranged in decreasing order are: habit of drinking non-boiled water, latrine not reaching the standard of hygiene, unappropriate treatment of children's feaces, using unhygienic rain water, well water, lake water.
risk factors ; child ; Diarrhea

10 strains of rotavirus C-1P6 causing acute diarrhoea in under 5- year - old - children were cultured on African Rhesus monkey kidney cells. Only 4 strains were adapted in the mixture of monkey MA104 kid cells. Results showed that in the most favorable medium, not all strains of virus could be adapted and grown properly. Rotaviruses of A group is the main agents causing acute diarrhoea in children in winter
Diarrhea ; Rotavirus ; Kidney ; child ;

By RT-PCR biomolecular technique on ARN isolated from 30% fecal mixture of 41 samples of under five years old children with acute diarrhoea due to rotavirus collected at Khanh Hoa Pediatric Hospital, the year 2002, 4 genotypes (G9, G1, G2 and G4), 3 P genotypes (P4, P6, P8) and 2 common phenotypes G1P8 and G2P4 were determined. While 9.75% of G gene and 21,95% of P gene were not determined yet
Diarrhea ; Rotavirus ; Child

Collecting stools sample from 232 children with acute diarrhoea, who treated in hospitals. Using diagnose immune enzyme EIA bio-product branch defined that reason of the disease is rotavirus. In 10 typies of rota G1P8 that cause of disease in Vietnam only have suitable and develop 4 typies in renal cell combination of Africa Rhesus monkey are G1P8 that signed 2001019423-11, 2001019427-15, 2001019428-17, 2001019432-20
Rotavirus ; Diarrhea ; Child

Study on 2483 children aged 0-5 years old with acute diarrhea at Ba Vi district (Ha Tay province), St Paul Hospital, and Thanh Tri district (Ha Noi). Results: prevalence of children aged 0-5 years old with acute diarrhea due to ETEC accounted for 6.4% of all diarrhea children. Majority of diarrhea children caused by ETEC alone (82.3%), most of ETEC diarrhea patients were due to LT ETEC (61.4%). ETEC-related diarrhea characterized loose stool, mild and moderate dehydration, fatigue (83.1%), anorexia (74.65%), and abdominal pain (70.6%). Disease developed quickly, with the mean time of 3.23±1.13 days. The prevalence is higher in summer and autumn.
Diarrhea ; Child ; Escherichia coli

Diarrhea continues to be one of the most common causes of morbidity and mortality among infants and children in developing countries. Escherichia coli are an emerging agent of pathogens causing diarrhea. Among 587 fecal samples from Vietnamese children under five years of age with diarrhea, the PCR technique identified 68 samples (11.6%) positive with EAEC and 18(7.2%) among the 249 age-matched controls showing the significant difference. In the diarrhea group, the significantly higher prevalence was seen in the children less than two years of age as compared to the older ones. There is a decreasing tend of EAEC infection during the age. When comparing the children at the age of less than 12 months, the prevalence of EAEC was significantly higher in the diarrhea group than in the control. More studies need to be carried out to verify the role of EAEC in causing diarrhea in children.
Diarrhea ; Escherichia coli ; Child