Twenty Rota virus strains were isolated from 20 stool specimens derived from diarrhea children in Khanh Hoa province. After 3 times of passaged culturations on MAI04 cells, there were only 2 human Rota virus strains growing well in the medium of trypsin concentration at 5 mg/ml and 4 human Rotavirus strains multiflying well in the medium of trypsin concentration at 10 mg/ml.
Rotavirus ; Diarrhea, Child ; Child

The study was performed on 10.585 household including 13.422 under 5 year old age children with 9.69% acquired acute diarrhoea and a mortality of 18.18% of cases. Risk factors ranged in decreasing order are: habit of drinking non-boiled water, latrine not reaching the standard of hygiene, unappropriate treatment of children's feaces, using unhygienic rain water, well water, lake water.
risk factors ; child ; Diarrhea

10 strains of rotavirus C-1P6 causing acute diarrhoea in under 5- year - old - children were cultured on African Rhesus monkey kidney cells. Only 4 strains were adapted in the mixture of monkey MA104 kid cells. Results showed that in the most favorable medium, not all strains of virus could be adapted and grown properly. Rotaviruses of A group is the main agents causing acute diarrhoea in children in winter
Diarrhea ; Rotavirus ; Kidney ; child ;

By RT-PCR biomolecular technique on ARN isolated from 30% fecal mixture of 41 samples of under five years old children with acute diarrhoea due to rotavirus collected at Khanh Hoa Pediatric Hospital, the year 2002, 4 genotypes (G9, G1, G2 and G4), 3 P genotypes (P4, P6, P8) and 2 common phenotypes G1P8 and G2P4 were determined. While 9.75% of G gene and 21,95% of P gene were not determined yet
Diarrhea ; Rotavirus ; Child

Collecting stools sample from 232 children with acute diarrhoea, who treated in hospitals. Using diagnose immune enzyme EIA bio-product branch defined that reason of the disease is rotavirus. In 10 typies of rota G1P8 that cause of disease in Vietnam only have suitable and develop 4 typies in renal cell combination of Africa Rhesus monkey are G1P8 that signed 2001019423-11, 2001019427-15, 2001019428-17, 2001019432-20
Rotavirus ; Diarrhea ; Child

Study on 2483 children aged 0-5 years old with acute diarrhea at Ba Vi district (Ha Tay province), St Paul Hospital, and Thanh Tri district (Ha Noi). Results: prevalence of children aged 0-5 years old with acute diarrhea due to ETEC accounted for 6.4% of all diarrhea children. Majority of diarrhea children caused by ETEC alone (82.3%), most of ETEC diarrhea patients were due to LT ETEC (61.4%). ETEC-related diarrhea characterized loose stool, mild and moderate dehydration, fatigue (83.1%), anorexia (74.65%), and abdominal pain (70.6%). Disease developed quickly, with the mean time of 3.23±1.13 days. The prevalence is higher in summer and autumn.
Diarrhea ; Child ; Escherichia coli

Diarrhea continues to be one of the most common causes of morbidity and mortality among infants and children in developing countries. Escherichia coli are an emerging agent of pathogens causing diarrhea. Among 587 fecal samples from Vietnamese children under five years of age with diarrhea, the PCR technique identified 68 samples (11.6%) positive with EAEC and 18(7.2%) among the 249 age-matched controls showing the significant difference. In the diarrhea group, the significantly higher prevalence was seen in the children less than two years of age as compared to the older ones. There is a decreasing tend of EAEC infection during the age. When comparing the children at the age of less than 12 months, the prevalence of EAEC was significantly higher in the diarrhea group than in the control. More studies need to be carried out to verify the role of EAEC in causing diarrhea in children.
Diarrhea ; Escherichia coli ; Child

From Aug 2001 to July 2002 at St Paul Hospital in Hanoi, 300 fecal samples collected from 300 diarrhoea children aged from 3 months to 5 years old were studied. Results showed that in 51,9% of cases, rotavirus was the leading cause, Escherichia coli was the second, among which addhesive strains and invasive strains of coli were the most common. In first time, toxic gene was detected in the group of intestinal hemorrhagic Escherichia coli. Shigella and Campillobacter were still the common pathologic causes of pediatric diarrhoae
Diarrhea/etiology ; Child ; Epidemiology

Background: Diarrhea disease is a common popular in children. It gives effect greatly to the development of children and also is a direct reason or indirect reason which results the mortality for children. Objectives:This study aims to assess the effectiveness of some interventional solution on diarrhea disease in children under 60 months of age at Hoang Tay, Kim Bang, Ha Nam. Subjects and method:An community intervention study (prospective longitudinal study) was undertaken from December 1994 to December 2000 in Kim bang district, Ha Nam province. Results: 956 children age from 0 to 60 and mothers have children under 60 months were kept under surveillance by two weeks once home visits within two years. The incidence sensitive of diarrhea in Hoang Tay (interventional commune) were 0.64 and 0.47 (effective indicator: 26.5%); in Van Xa (control group) were 0.68 and 0.63 (effective indicator: 7.3%);interventional effectiveness: 19.2%. After intervention, at Hoang Tay village, the mothers use ORS for treatment increase 58.2%, use porridge extraction increase 116.6% and use antibiotic reduced 8.8%, use herbal medicine reduced 30.8%. Conclusion: The incidence density of diarrhea disease at Hoang Tay was reduced by some interventional solutions, interventional effectiveness: 19.2%.
Diarrhea/ therapy ; Child

A case-control study was realized at children hospital No 1 Ho Chi Minh City, Vietnam, in order to identify the risk factors of diarrhoeal infants under 3 years of age. The cases were hospitalized with persistent diarrhoeal diagnosis according to World Health Organization standards. The controls were hospitalized cases without any diarrhoeal symptoms, being the same age and admitted to hospital at the same time as the case. The sample size was 140 cases and 164 controls. At the moment of inclusion, all the subjects were interviewed, examined and all the information about them were recorded in questionnaire forms by the well trained doctors. Epi info package was used for invariable analysis and Egret for multiple logistic regression, 37 factors were studied, but at the end of the analysis, only 6 factors were associated significantly with persistent diarrhea
Diarrhea ; Child ; Risk factors