Twenty Rota virus strains were isolated from 20 stool specimens derived from diarrhea children in Khanh Hoa province. After 3 times of passaged culturations on MAI04 cells, there were only 2 human Rota virus strains growing well in the medium of trypsin concentration at 5 mg/ml and 4 human Rotavirus strains multiflying well in the medium of trypsin concentration at 10 mg/ml.
Rotavirus ; Diarrhea, Child ; Child

Diarrhea continues to be one of the most common causes of morbidity and mortality among infants and children in developing countries. Escherichia coli are an emerging agent of pathogens causing diarrhea. Among 587 fecal samples from Vietnamese children under five years of age with diarrhea, the PCR technique identified 68 samples (11.6%) positive with EAEC and 18(7.2%) among the 249 age-matched controls showing the significant difference. In the diarrhea group, the significantly higher prevalence was seen in the children less than two years of age as compared to the older ones. There is a decreasing tend of EAEC infection during the age. When comparing the children at the age of less than 12 months, the prevalence of EAEC was significantly higher in the diarrhea group than in the control. More studies need to be carried out to verify the role of EAEC in causing diarrhea in children.
Diarrhea ; Escherichia coli ; Child

Chronic diarrhea is defined as passing watery stools that lasts for more than 2 weeks. Persistent diarrhea belongs to chronic diarrhea and is a chronic episode of diarrhea of infectious etiology. The etiology of chronic diarrhea is varied. It is important to consider the child's age and clinical manifestations with alarm signals for an application of proper treatments to children with chronic diarrhea. Vicious cycle is present in chronic diarrhea and nutritional rehabilitation can break the vicious cycle of chronic diarrhea and is one of the main one thing among treatments. We should know the exact concept of chronic diarrhea and provide appropriate treatments according to etiologies of chronic diarrhea.
Child ; Diarrhea ; Humans

From Aug 2001 to July 2002 at St Paul Hospital in Hanoi, 300 fecal samples collected from 300 diarrhoea children aged from 3 months to 5 years old were studied. Results showed that in 51,9% of cases, rotavirus was the leading cause, Escherichia coli was the second, among which addhesive strains and invasive strains of coli were the most common. In first time, toxic gene was detected in the group of intestinal hemorrhagic Escherichia coli. Shigella and Campillobacter were still the common pathologic causes of pediatric diarrhoae
Diarrhea/etiology ; Child ; Epidemiology

Background: Diarrhea disease is a common popular in children. It gives effect greatly to the development of children and also is a direct reason or indirect reason which results the mortality for children. Objectives:This study aims to assess the effectiveness of some interventional solution on diarrhea disease in children under 60 months of age at Hoang Tay, Kim Bang, Ha Nam. Subjects and method:An community intervention study (prospective longitudinal study) was undertaken from December 1994 to December 2000 in Kim bang district, Ha Nam province. Results: 956 children age from 0 to 60 and mothers have children under 60 months were kept under surveillance by two weeks once home visits within two years. The incidence sensitive of diarrhea in Hoang Tay (interventional commune) were 0.64 and 0.47 (effective indicator: 26.5%); in Van Xa (control group) were 0.68 and 0.63 (effective indicator: 7.3%);interventional effectiveness: 19.2%. After intervention, at Hoang Tay village, the mothers use ORS for treatment increase 58.2%, use porridge extraction increase 116.6% and use antibiotic reduced 8.8%, use herbal medicine reduced 30.8%. Conclusion: The incidence density of diarrhea disease at Hoang Tay was reduced by some interventional solutions, interventional effectiveness: 19.2%.
Diarrhea/ therapy ; Child

A case-control study was realized at children hospital No 1 Ho Chi Minh City, Vietnam, in order to identify the risk factors of diarrhoeal infants under 3 years of age. The cases were hospitalized with persistent diarrhoeal diagnosis according to World Health Organization standards. The controls were hospitalized cases without any diarrhoeal symptoms, being the same age and admitted to hospital at the same time as the case. The sample size was 140 cases and 164 controls. At the moment of inclusion, all the subjects were interviewed, examined and all the information about them were recorded in questionnaire forms by the well trained doctors. Epi info package was used for invariable analysis and Egret for multiple logistic regression, 37 factors were studied, but at the end of the analysis, only 6 factors were associated significantly with persistent diarrhea
Diarrhea ; Child ; Risk factors

The diarrhea is common and major cause of the mortality in the newborn in developing countries. Rotavirus was most frequent pathogen of diarrhea, accounted for 20-70% of cases of severe diarrhea that were admitted to hospital. It was necessary to have the vaccine against rotavirus for preventing from rotavirus diarrhea. WHO encouraged the development and application of vaccine for prevention and control the Rotavirus over the world
child ; Diarrhea ; Rotavirus

To identify the viral cause in children with acute diarrhoea in §ucTrong hospital - LamDong province, 158 stool specimens of children with Acute diarrhoea have been collected and identified for rotavirus, adenovirus and snow mountain virus. The result were compared with 151 stool specimens of healthy children in the same time for 3 years (1994-1997). Results of the study showed: the viral positive was found in 52.2% of the diarrheal group. While it was 1.3% in the Healthy Control Group. Among the viral positive, the rotavirus is 50%, Astrovirus is 1.26%, snow mountain virus is 0.67% and adeno virus is 0.63%. For three years, three types of rotavirus has been identified in DucTrong, which are: Rotavirus type I (23/79), Rotavirus type II (13/79), rotavirus type IV (21/79) undifferent (22/79). The rotavirus type III has not been found in this time. The clinical characteristic of the viral diarrheal group has been found in 94.4% of children under 2 years, 81% of cases had vomiting. The dehydration including degree B and C according to the classification of WHO is from 84.74% to 95.34%.
diagnosis ; Diarrhea ; child ; Viruses

A descriptive study with analysis on 371 interview papers was implemented in Khoai Chau District has shown that the children are not sucked fully breast milk (OR= 5,53), the children are sucked frequently breast milk (OR= 3,09), the children are received the chewed cooked rice (OR= 2,88). The water resource without hygiene (OR=6,23). unhygienic toilet (OR= 3,28), children with birth weight under 2500g (OR= 426). The mother who do not receive the communication of diarrhea (OR= 2,1).
diarrhea ; child ; risk factors

This study was applied for 1,729 children under 5 years of age selected randomly from Cai Be district, Tien Giang province by 30 cluster method. The overall incidence was 40.5 episodes/100 infants/years, it was highest for infants under 6 months of age (96 episodes/100 infants/years), followed by infants 6 to 11 months of age (84 episodes/100 infants/years). After 24 months of age, it decreased obviously. The duration of diarrhea: the majority of diarrhea episodes were short duration, 86% of episodes lasted from 1 to 4 days, only 0.5% lasted over 14 days. Risk factors associated to the prolongation of diarrhea were: infants under 1 year of age, liquid stool with defecation more than 5 times in 24 hour, bloody stool, using antibiotics more than 5 days, malnutrition and beginning of mixing feeding.
diarrhea ; risk factors ; child