AIM: To investigate the effect of hyperhomo cysteine on endothelial cell function. METHODS: By establishing hyperhomocysteinemia model, 18 male New Zealand rabbits were divided into control group (control group) and high-methi o nine-diet group (M group). At the end of 3 weeks, M group was divided again into M+0 group (continuing high methionine-diet) and M+F group (high-methionine-diet plus folic acid, vitamin B 12). At the end of 6 weeks, isolated aortic rin g s were made and the maximum vasodilation of the aortic rings to Ach was investig ated. Meanwhile, the plasma concentrations of Hcy, NO, ET-1, Ang II at 0 week, 3 weeks and 6 weeks and the contents of NO 2-/NO 3-, Ang Ⅱ, ET-1, NOS i n regional vascular tissue at 8 weeks were also measured. RESULTS: (1) In contrast to M+F group and control group, the max imum vasodilation to Ach were decreased (E max=26.73?4.51 vs 47.84 ?5.62, 56.42?7.82, P

Objective By exploring the alteration of tissue plasminogen activator (t-PA) and tissue plasminogen activator inhibitor-1 (PAI-l) in plasma in Kawasaki disease (KD) patients to identify the pathophysiological mechanism of vascular damage and to detect the relationship between fibrinolytic system function and coronary artery lesion complications in Kawasaki disease patients. Method Plasma levels of t-PA, PAI-1 antigen were measured by enzyme-linked immunosorbent assay. Patients with KD were grouped into acute phase group, recovery phase group patients with coronary artery lesion group and non-coronary artery lesion group. Results Plasma t-PA and PAI-1 in acute phase and recovery phase groups were significantly higher than those in the healthy group (P