1.The expression and significance of oncogene C-erbB-2,p16,nm23H1 in exhepatic bile duct cancer
Yanbin WANG ; Ningxin ZHOU ; Dianjun WANG
Chinese Journal of General Surgery 2001;0(08):-
Objective This study was to investigate correlations between the expression of oncogene c erbB 2 , p16 and nm23H1 and the development of the carcinoma. Methods Immunohistochemical S P method was used to examine the expression of oncogenes in 46 cholangiocarcinoma cases and 13 cases of benign bile duct lesions. The relationship between the expression and clinical pathology was analyzed. Results The positive rate of C erbB 2 in cholangiocarcinoma increased significantly ( P
2.The Biological Characteristics of IL-2 Gene Modified Tumor Cells
Yihua SUN ; Dianjun LI ; Zhihua WANG
Chinese Journal of Cancer Biotherapy 1994;0(01):-
In this experiment, we introduced human interleukin-2(lL-2)gene and Neomycin resistance (Neo~R) gene into a human lung adenocarcinoma cell line GLC, using a retroviral vector PLXSN. The positive cells obtained by selecting in G418 were compared with the wild-type cells. We observed their growth characteristics in vitro and the quantity of IL-2 in the culture supernatants. PCR result demonstrated the integration of foreign genes into tumor genome DNA after transfection and permanent existence. Under electron microscope, what can be seen was the surface villi of trans-duced-cells are less and shorter. It deserves further investigation to determine whether the change of cell surface influence metastatic ability of cells.
3.Studies on the intratumoral microvascular density (IMD) and the VEGF expression in malignant mesothelioma
Jie GAO ; Guo YU ; Dianjun WANG
Medical Journal of Chinese People's Liberation Army 2001;0(12):-
Objective To study the prognosis significance of intratumoral microvascular density(IMD)and the expression of vascular endothelial growth factor(VEGF)in malignant mesothelioma.Methods The data of 79 cases were reviewed of malignant mesothelioma confirmed by histology,immunohistochemistry and telectron microscope.CD34 and the expression of VEGF were detected by Immunohistochemical method.Results The age of the patients with malignant mesothelioma ranged from 11 to 79 years.The peak incidence of malignant mesothelioma was at the range of 40-50 years.The sex ratio of male to female was 1.78∶1.The survival period of the 79 cases was between 5 and 160 months with the medial survival of 23 months.According to histological structure,these cases were divided into epithelioid type(51 cases),sarcomatous type(20 cases)and mixed type(8 cases).For the VEGF positive expression,epithelioid type was 26 cases(50.98%),sarcomatous type was 13 cases(65.00)and mixed type was 5 cases(62.50%).Based on the mean IMD,the patients with malignant mesothelioma were divided into two groups.The survival time of the patients in the higher IMD group was significantly shorter than those in the lower IMD group.The analysis,on the relation of VEGF expression to IMD,as well as survival time and histological subtype,showed that VEGF expression was positively correlated to IMD(P=0.02,r=0.330 2).A significant difference existed between the survival of VEGF positive expression and negative expression(P0.05).Conclusion The results suggest that the prognosis of malignant mesothelioma probably related with IMD and VEGF expression.The higher index of IMD and the higher expression of VEGF might imply a worse prognosis.
4.The experimental study on antitumor effect of B7-1 gene modified murine EL-4 cells vaccine
Qingyuan ZHANG ; Dianjun LI ; Zhihua WANG
Chinese Journal of Immunology 1985;0(05):-
Objective:To study the vaccine potency of gene-modified tumor cells. Methods:The EL-4 lymphoma was transduced with recombinant retrovirus containing the murine B7-1 gene. The effect of gene transduction on antitumor immunity was investigated.Results:The appearance, growth rate and surface marker of MHCⅠand MHCⅡ molecules of EL-4 cells transduced with B7-1 gene were the same with control cells except for CD80 positive in B7-1 gene transduced cells. B7-1 gene transduced EL-4 cells resulted in remarkable loss of tumorigenicity in syngenetic mice. EL-4/B7-1 cells could induce system protective immunity. Therapeutic vaccine of EL-4/B7-1 cells could retard the growth of established early-stage EL-4/Wt tumor significantly, but not retard the growth of late-stage EL-4/Wt tumor. Irradiated EL-4/B7-1 vaccine showed weak effect against challenged EL-4 cells.Conclusion:B7-1 gene transduced EL-4 cells can induce system protective immunity. It suggested that this vaccine have a potential application value in human cancer treatment.
5.Role of PERK/eIF2α signaling pathway in systemic damages resulted from fluorosis
Shize WANG ; Wei WEI ; Dianjun SUN
Chinese Journal of Endemiology 2016;35(9):698-702
Fluoride can cause phrenology and non-phrenology damage,but the mechanisms were unclear.It is reported that fluoride can decrease protein synthesis and induce cell apoptosis,leading to extensive systemic damage.Many studies have found that the mechanism is closely associated with endoplasmic reticulum stress.Protein kinase receptor-like ER kinase/Eukaryotic translation initiation factor 2α (PERK/eIF2α) signaling pathway is the first activation pathway when endoplasmic reticulum stress occurs which may play an important role in the pathogenesis of fluorosis.This present paper is focused on the role of PERK/elF2α signaling pathway-related factors in the systemic and organism damages resulted from fluorosis,which may provide new ideas in mechanism and prevention of fluorosis.
6.Synthesis and Applications of Gold Nanoparticle Probes
Lina MA ; Dianjun LIU ; Zhenxin WANG
Chinese Journal of Analytical Chemistry 2010;38(1):1-7
During last decade, gold nanoparticled (AuNPs)-based assays have been well-developed and widely used in biological analysis and biomedical detection because AuNPs have unique physical and chemical properties which depend on the size, shape and degree of aggregation.The AuNPs-based assays have already been employed for detecting practical samples with high simplicity and selectivity.This review discusses the recently development of the synthesis and biological molecular functionalisation of AuNPs and their applications on the heavy metallic cations, small organic compounds, nucleic acids and proteins detection and cellular analysis.
7.Effect of fimH gene on type 1 fimbriae adhesion of uropathogenic Escherichia coli: a preliminary study
Jianfei WANG ; Yang CAO ; Dianjun WEI
Chinese Journal of Clinical Infectious Diseases 2013;6(5):278-281
Objective To investigate the effect of fimH gene on type 1 fimbriae adhesion of uropathogenic Escherichia coli (UPEC) and the gene variations between type 1 fimbriae adhesion positive and negative bacteria.Methods A total of 171 UPEC strains (not catheter associated) were collected from the Second Hospital of Tianjin Medical University,Tianjin First Center Hospital,and Tianjin Children's Hospital during January 2012 and January 2013.fimH gene was detected by PCR technique,and type 1 fimbriae adhesion was detected by yeast agglutination test.RT-PCR was used to exterminate gene transcript factor impacting on adhesion.Chi-square and Yates' chi-squared tests were performed to comparefimH gene sequences between the adhesion positive and negative bacteria.Results Among 171 UPEC strains,142 (83%) werefimH gene positive,and type 1 fimbriae was expressed in 98 strains (57%).All adhesion positive strains carriedfimH gene.Among 44 strains with positivefimH gene and negative adhesion RT-PCR revealed thatfimH gene did not transcript in 8 strains (18%).The sequencing results showed that gene mutation on the 51 st amino acid site was more prevalent in the adhesion positive group compared with the negative group (x2 =6.64,P =0.010).In adhesion,mutations on the 190th and 219th amino acid sites were observed in 6 strains and 7 strains of negative group,respectively; while not observed in the adhesion positive group (x2 =4.69 and 5.87,P < 0.05).Negative adhesion in other 23 strains was not attributed to single nucleotide polymorphism.Conclusion Adhesion function of type 1 fimbriae mignt be affected by mutation and deletion offimH gene.Three key site mutations may also affect the adhesion function of type 1 fimbriae.Besides fimH gene,there may be other genes that can affect the adhesion function of type 1 fimbriae.
8.Gold Nanoparticle-based Dynamic Light Scattering Assay for Detection of Mercury Ion
Lina MA ; Dianjun LIU ; Zhenxin WANG
Chinese Journal of Analytical Chemistry 2014;(3):332-336
A gold nanoparticle ( GNP)-based dynamic light scattering ( DLS) assay has been developed for detecting mercury ion ( Hg2+) in aqueous solution. The GNPs are able to maintain monodisperse state in relative high ionic strength ( 0. 1 mol/L NaCl ) aqueous solution because Hg2+ aptamer 5’-TTTCTTCTTTCTTCCCCCCTTGTTTGTTGTTT-3’( Probe DNA ) can adsorb on the GNP surface by electrostatic interaction. In the presence of Hg2+, the Probe DNA molecules easily desorb from GNP surface because of T-Hg2+-T formation. The unprotected GNPs form aggregates in 100 mmol/L NaCl solution, resulting in increased significantly the average hydrodynamic diameter of GNPs. Under the conditions of pH 7. 43, 110 nmol/L aptamer, 100 mmol/L NaCl and 30 minutes incubation time of Hg2+with aptamer, the difference of average hydrodynamic diameter (△D ) of GNPs was increased linearly with logarithm of Hg2+concentration over the range from 0. 1 nmol/L to 5 μmol/L, with a detection limit of 0. 1 nmol/L. Moreover, satisfactory results were obtained when the proposed method was applied to detect Hg2+ in real samples.
9.Schistosomiasis control effect of agricultural integrated development in Tie-ban Marshland of Yangtze River
Dianjun WANG ; Lecheng HAN ; Yijun CAO ; Hanzhou WANG
Chinese Journal of Schistosomiasis Control 2016;28(3):338-339
Objective To evaluate the schistosomiasis control effect of the comprehensive measures that focused on beach?land smoothing and tillage cultivation in Tieban Marshland,Wuhan Section of the Yangtze River. Methods From 2011 to 2015, before and after the comprehensive control,the Oncomelania hupensis snail situations in Tieban Marshland were investigated in spring annually,and the residents aged 6-65 years in Huayuan Community which was next to the marshland were examined to un?derstand their schistosome infection status. In late July,2014,the sentinel mouse surveillance was carried out. Moreover,the eco?nomic effectiveness of comprehensive development was evaluated. Results The schistosome infection rates of residents in 2011 (before the comprehensive control),2012(after the comprehensive control),and 2013 were 0.72%(3/414),0.37%(2/536)and 0.31%(1/326),respectively,and no schistosomiasis patients were found in 2014 and 2015. The snail area,the occurrence rate of frames with snails,and the average density of living snails in Tieban Marshland in 2005 decreased by 22.18%,97.83% and 98.25%,respectively compared to those in 2011,and no schistosome infected snails were found. No positive mice were found in the sentinel mouse surveillance. The annual net income of Tieban Marshland increased by 233.33%compared to that before the comprehensive development. Conclusion The endemic situation of schistosomiasis and snail situation declined significantly af?ter the comprehensive development in Tieban Marshland,and the economic and social benefits are significant.
10.An assay for the determination of alkaline phosphatase activity using disodium p-acetylphenylphosphate as a substrate
Jianping CAO ; Xiaoqin DING ; Ling WANG ; Dianjun WEI
Chinese Journal of Clinical Laboratory Science 2006;0(05):-
Objective To establish a new method to measure alkaline phosphatase (ALP) activity using disodium p-acetylphenylphosphate (PAP-PNa2) as substrate.Methods Experimental parameters were set up with continuous-monitoring procedures on a semiautomatic analyzer.Results The wavelength of maximum absorption of PAP was 325 nm,and the Km of ALP was 0.376 mmol/L.The molecular extinction coefficient of PAP at 340 nm was 23 390 L?mol-1?cm-1 and the concentration of citrate buffer was 0.438 mol/L.In the measurement,the optimum pH was 10.4,and the optimum concentration of substrate was 5.0 mmol/L.The delay time was 60 sec and the time keeping the linear reaction was 15 min,and the linear range was 0~1 110 U/L.The measurement was not interfered by the hemoglobin