ObjectiveTo investigate the predictor and prophylaxis of cardiovascular events in diabetic nephropathy (DN) patients. MethodsFifty-eight cases of DN patients were assigned randomizedly to two groups. Conventional treatment group(33 cases) received conventional treatment, and ginkgo biloba extract group(30 cases) received extra ginkgo biloba extract capsules treatment. Plasma examination for blood fat, blood pressure(Bp), brain natriuretic peptide(BNP) and echocardiogram was performed every 3 months. All the patients were followed-up for the occurrence of cardiovascular events. ResultsThere were no significant differences in age, sex, Bp, blood fat, serum creatinine, hemoglobin,BNP, left ventricular E/A and left ventricular ejection fraction (LVEF) in two groups at the time of recruitment. After treatment of 12 months, the level of plasma BNP decreased significantly in ginkgo biloba extract group and maintained in the lower levels throughout the follow-up compared with that in conventional treatment group(P＜ 0.01 ). After treatment of 18 and 24 months, left ventricular E/A and LVEF were better in ginkgo biloba extract group than those in conventional treatment group(P ＜ 0.01 or ＜ 0.05 ). In ginkgo biloba extract group, the level of plasma BNP after treatment of 12 months had correlation with left ventricular E/A and LVEF after treatment of 24 months (r =0.61, -0.68, P ＜ 0.05 ). Survival analysis indicated that the level of plasma BNP was a predictor of cardiovascular event in DN patients. ConclusionThe level of plasma BNP can be used as a predictor of cardiovascular event in DN patients,and ginkgo biloba extract capsules can decrease the level of plasma BNP and reduce the incidence of cardiovascular events in DN patients.

During last decade, gold nanoparticled (AuNPs)-based assays have been well-developed and widely used in biological analysis and biomedical detection because AuNPs have unique physical and chemical properties which depend on the size, shape and degree of aggregation.The AuNPs-based assays have already been employed for detecting practical samples with high simplicity and selectivity.This review discusses the recently development of the synthesis and biological molecular functionalisation of AuNPs and their applications on the heavy metallic cations, small organic compounds, nucleic acids and proteins detection and cellular analysis.

A gold nanoparticle ( GNP)-based dynamic light scattering ( DLS) assay has been developed for detecting mercury ion ( Hg2+) in aqueous solution. The GNPs are able to maintain monodisperse state in relative high ionic strength ( 0. 1 mol/L NaCl ) aqueous solution because Hg2+ aptamer 5’-TTTCTTCTTTCTTCCCCCCTTGTTTGTTGTTT-3’( Probe DNA ) can adsorb on the GNP surface by electrostatic interaction. In the presence of Hg2+, the Probe DNA molecules easily desorb from GNP surface because of T-Hg2+-T formation. The unprotected GNPs form aggregates in 100 mmol/L NaCl solution, resulting in increased significantly the average hydrodynamic diameter of GNPs. Under the conditions of pH 7. 43, 110 nmol/L aptamer, 100 mmol/L NaCl and 30 minutes incubation time of Hg2+with aptamer, the difference of average hydrodynamic diameter (△D ) of GNPs was increased linearly with logarithm of Hg2+concentration over the range from 0. 1 nmol/L to 5 μmol/L, with a detection limit of 0. 1 nmol/L. Moreover, satisfactory results were obtained when the proposed method was applied to detect Hg2+ in real samples.

Objectives To analyze and compare four correction methods for measuring thyroid volume,and to find out the best one for thyroid volume correction of children aged 8-10.Methods In 2012 and 2013,the project was carried out in iodine deficient rural areas (water iodine below 10 μg/L) of Jilin and Gansu Provinces,10 villages in each province were chosen as survey sites,and 40 children of 8 to 10 years old were chosen from each village,half boys and half girls.Utrasonography was adopted to determine the thyroid volume,and height and weight were measured.The thyroid volume was corrected by Body Mass Indicator(BMI),weight and height,body surface,and height,separately.Body Mass Indicator corrected Volume (BMIV),Weight and Height corrected Volume Indicator(WHVI),Body Surface Area corrected Volume(BSAV) and Height corrected Volume Indicator(HVI) were calculated and compared with their original thyroid volumes to find out the best one.Results Datas of 450 and 408 children were collected from Jilin and Gansu Provinces,separately.According to their original thyroid volumes,goiter rates of Jilin and Gansu were 10.4％ (47/450) and 0.5％ (2/408),respectively.All these four methods could decrease the difference between provinces and sexes.Among the four methods,WHIV and BSAV were abnormal for correction of different ages,heights and weights.HVI had the most normalized distribution and stronger correlation with the original thyroid volumes than others(r =0.961).The HVI corrected results had similar distribution with the original thyroid volume but the data were different.After being adjusted by 1.7 × HVI,the results were close to the original thyroid volumes.Goiters in Jilin and Gansu Provinces,were 9.1％(41/450) and 0.5％(2/408),respectively,after correction.Conclusion The 1.7 × HVI is the most suitable method for correcting the thyroid volume of children aged 8-10.

Objectives To study the changes of thyroid function under different urinary iodine levels and pregnant trimesters,and to investigate the urinary iodine standard of pregnant women,taken thyroid function as a golden standard.Methods Early,mid and late trimester pregnant women in the iodine deficient rural areas of Gansu and Jilin Provinces were selected,at least 70 cases were included for each trimester in each province.Pregnant women included were physically healthy;cases should be excluded if their immediate relatives had a thyroid disease history.Urinary iodine,free triiodothyronine (FT3),free thyroxine (FT4),thyroid stimulating hormone (TSH),thyroid peroxidase antibody (TPOAb),thyroglobulin antibody (TgAb),and edible salt iodine from pregnant women's home were tested.The urinary iodine was measured with the method for determination of iodine in urine by As3+-Ce4+ catalytic spectrophotometry (WS/T 107-2006).Thyroid function was tested by chemiluminescence method,salt iodine was determined by direct titration method (GB/T 13025.7-1999).Results Totally 439 pregnant women were investigated in the two provinces.Median of urine iodine was 178.6 μg/L;for the 215 women in Gansu,the value was 156.4 μg/L;for the 224 women in Jilin,the value was 206.4 μg/L.Along with the increase of urinary iodine,the FT3 of Gansu decreased from 5.77 pmol/L to 5.34 pmol/L,TSH increased from the lowest 2.63 mU/L to 3.75 mU/L;the FT3 of Jilin increased from 4.41 pmol/L to 4.45 pmol/L,TSH increased from the lowest 0.92 mU/L to 1.50 mU/L.Five indicators of thyroid function of more than 97％ pregnant women were in normal range and FT3 was the most stable in the three trimesters.FT3 of the three triimesters in Gansu was 6.27,5.76,5.28 pmol/L,respectively,and that in Jilin was 4.50,4.42,4.32 pmol/L,respectively.The 5％ and 95％ quantile were 55.24,581.27 μg/L of urinary iodine,of pregnant women with normal thyroid function.Conclusions Under different urinary iodine levels,pregnant women's thyroid function is relatively stable.TSH has showed an increasing trend with increasing iodine level.Along with the progress of pregnancy,the urinary iodine is different among the three pregnancy periods,but the thyroid function is stable.The pregnant women with normal thyroid function have a urinary iodine level between 50-600 μg/L.

0.05).Thirty genotypes were got from 54 strains by ERIC-PCR.CONCLUSIONS There isn′t significant difference on the absence rate of oprD2 gene in Tianjin and it gives us a hint that other factors can also result in the prevalence of IRPA.The existence of prevalence of IRPA clone in some departments advises us to effectively control the IRPA in hospital.

Objective To examine the detection rate of 30 known virulence factors (VFs) of extraintestinal pathogenic Escherichia coli(ExPEC), and to investigates the epidemiology of ExPEC in elderly nosocomial infection. Methods A to?tal of 140 ExPEC clinical isolates from elderly nosocomial patients in hospitals in Tianjin were investigated. Multiplex PCR was performed to detect the 30 virulence factors among the E.coli strains and the detection rate of virulence factors for Ex?PEC were compared between isolates from different sites of infection.Fifty E. coli strains were shown to carry fimH gene that was amplified and sequenced. These sequences were used besides3 references strains (CFT037、UTI89 and K-12 ) to detect SNPs of fimH gene using DNAMAN Version 6.0.3.93 these 53 fimH sequences were used for genotyping and building dendrogram by MEGA4 software. Results In ExPEC, the following virulence factor genes, fimH, traT, fyuA, iutA and kpsMT II, had a higher detection rate than those of the rest . The following virulence factor genes, kpsMT II, K5, papC, pa?pEF ,papG allele II (Internal), papA, cnf1 (CNF), sfa/focDE and rfc had a a higher detectionrate from non-urine origin sam?ples than those from urine origin samples. fimH SNPs analysis of the 50 clinical isolated samples and 3 references samples showed 60 SNPs at 57 polymorphic sites. The fimH SNPs analysis classified the 53 strains into 25 genotype. The genetic fin?gerprintings of 11 isolates were exactly the same. Conclusion Many kinds of virulence factors can be found in ExPEC of el?derly nosocomial infection. The ExPEC strain isolated from non-urine origin had a stronger pathogenicity than those from urine-origin specimens. fimH SNPs analysis is suitable for molecular epidemiological investigation of ExPEC in hospital.

Objective:This article analyzes the epidemic situation and characteristics of Corona virus disease 2019 (COVID-19) in Russian Federation (referred to as Russia), summarizes the effective measures and problems exposed by Russia to deal with COVID-19, so as to provide reference for our country's epidemic prevention and control, and seek the direction of cooperation under the background of Sino Russia scientific and technological innovation in view of public health emergency.Methods:The epidemic characteristics and prevention and control measures were analyzed based on the data released by official authoritative news media such as Sputnik News Agency & Radio and Stopcoronavirus Website.Results:Russia's first confirmed case was on January 31, 2020 and its first peak of epidemic outbreak was on May 10, 2020. Thanks to a series of prevention and control measures and isolation and detection systems established by the Russia government according with national conditions, such as establishment of COVID-19 medical treatment centers, restrictions on alcohol sales, and the accelerating development of the vaccine and test kit the epidemic was basically controlled at the end of August in 2020. In September, Russia saw the second peak of the outbreak of COVID-19.Conclusions:The fatality rate of COVID-19 in Russia has been at a low level. Therefore, its prevention and control measures, experience and even its deficiencies are worth of learning by China. And we should also strengthen cooperation with Russia in the field of vaccine research and development and its clinical trials.

Objective: To study the biological characteristics of tumor cells infected with recombinant adenovirus expressing hTNF-?, investigate the antitumor effect of recombinant adenovirus. Methods: Human lung adenocarcinoma cell line Anip973 was infected with recombinant adenovirus expressing hTNF-?. Cell growth assay, colone formation test, flowcytometry assay and morphology were used to observe the effects on tumor cells. The hTNF-a gene, which was transduced into cancer cells mediated by recombinant adenovirus, was detected by PCR and agarose gel electrophoresis and its products were detected by ELISA assay. The intratumoral injection of rAd-LacZ and rAd-hTNF-? was carried out to evaluate their antitumor effects. Results: The liter of rAd reached 1010 PFU/ml and more than 90% Anip973 cells could be infected by 30MOI rAd. Except the surface structure and ultrastructure of tumor cells infected with rAd had a light change, cell growth abillity assay, colone formation test, flow cytometry assay showed no significant difference compared with that of the control cells. The TNF-? gene expression at 24 h increased greatly. Antitumor study indicated that on the tumor-bearing mice treated with rAd the tumor grew slowly. Tumor volume was significantly smaller and survive time was prolonged than that of controls. Conclusion: There was no significantly changes occurred on tumoral cells after infected with recombinant adenovirus expressing hTNF-?. The intratumoral injection of rAd-LacZ and rAd-hTNF-? could inhibit the growth of solid tumor.

Objective To investigate dynamic changes of plasma D-dimer, C-reactive protein (CRP) and fasting plas?ma glucose (FBG) levels in elderly patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) combined with type 2 diabetes mellitus. Methods AECOPD group (n=65) was used as A group, AECOPD combined with type 2 diabetes mellitus group (n=65) was used as B group. Levels of D-dimer, CRP and FBG were observed and compared between two groups in the first day, the 3rd day, the 7th day and the 14th day after hospital admission. The correlation be?tween D-dimer, CRP and FBG was analyzed. Logistic regression analysis was used to estimate the factors affecting increased plasma levels of D-dimer. Results Levels of D-dimer, CRP and FBG were significantly higher in B group than those of A group in the first day, the 3rd day, the 7th after hospital admission (P<0.01). Values of D-dimer and CRP were declined ob?viously in A group in the 7th day after hospital admission, but in B group they were declined obviously until the 14th day. There was no significant difference in FBG of A group. The level of FBG was significantly decreased in B group. There was a positive correlation between D-dimer, CRP and FBG (P<0.05). Logistic regression analysis showed that CRP, FBG, p(O2) and age were the influence factors for the increased levels of D-dimer. Conclusion In elderly patients with AECOPD com?bined with type 2 diabetes mellitus, we should pay attention to anti-inflammatory and controlling blood sugar, and the dy?namic monitoring levels of D-dimer, which helps to improve the prognosis for patients with AECOPD, and reduce mortality.