After McCabe's study about autoimmunization of perception hearing loss in 1979, the immunotest was interested in, especially the antibody neutrophil cytoplasmic antibody test (ANCA) for detecting autoimmune cause. The treatment by immunodepression presents a good result. Authors studied two patients with perception hearing loss, and with the test ANCA(+), who were treated by Imurel and corticoid
Diagnostic Techniques, Otological ; diagnosis

Ultrasound biomicroscopy (UBM) has been applied in ophthalmology for twenty years. It plays an important role in diagnosis of eye diseases, especially in glaucoma. After years of research, It has been developed in its structure, sensors, imaging technology and applied research.
Diagnostic Techniques, Ophthalmological ; Microscopy, Acoustic

Fasciola eggs were collected from stool of a woman aged 21 years old in Nghe An province. Miracidium were obtained from these eggs after 20 days culture in the laboratory. A portion of the mitochondrial-encoded nad1 gene from these miracidiums were amplified using polymerase chain reaction (PCR) and comparatively aligned with the known corresponding sequences of Fasciola gigantica taken from cattle in Hoa Binh, Lai Chau, Lang Son, Ninh Binh and international strains (from Korea, Japan and Indonesia). The results showed high similar co-efficient of nucleotide 98-99% and amino acid 95-99%, but lower rate than that of Fasciola hepatica (Australian and American) (90-92%). However, it is suggested that the Vietnamese F.gigantica have had the genetic signs hybridized with F.hepatica
Women ; diagnosis ; Ovum ; Fasciola ; Molecular Diagnostic Techniques

By molecular identification and using mitochondrial genetic markers, the results showed that different forms of lung fluke in Vietnam, including adult fluke from human, dogs, cats and Potamicus sp. rock crab, have been identified as Paragonimus heteotremus. Molecular-based analysis on 390 nucleotides of the cytochrome oxidase gene revealed that Paragonimus heterotremus of Vietnam from all forms showed high identity to the Chinese and Thai strains. (99.0 -99.2% nucleotide and 97-100% amino acid). Phylogenetic analysis uniquely placed the Vietnamese Paragonimus sp to the group of P.heterotremus of Chinese and Thai origin. Thus, P.heterotremus is offically identified from most of the natural and experimental hosts in Vietnam
Molecular Diagnostic Techniques ; Paragonimus ; Genome, Mitochondrial

284 patients with pleural effusion (PE) treated in A.D of Bach Mai Hospital from 1 Jan to 31 Dec 2001 were investigated retrospectively. The causes of PE weres determined as tuberculosis 32.7%, lung cancer 23.9%, parapneumonic effusion 8.1%, heart failure 7%, liver cirrhosis 3.5% and other causes 6.7%. 77.9% of cancer pleural effusion were aged above 50 and 66.7% of tuberculosis pleural effusion – under 50, common signs and symptoms were chest pain 76.7%, dypsnea 78.2%, cough 46.8%, expectoration 27.8%, fever 50.4%, weight loss 29.9% and pleural effusion syndrom 87%. Parachinical characteristics were: By chest Xray, free effusion in 78.6%. By pleural ultrasound, free pleural effusion 63.7%; lobulated pleural effusion 13.1%; pleural effusion with walls 20.2%. Rivalta test of pleural fluid(+) 83.8%, (-) 16.2%. Cytology of pleural fluid: malignant cells found in 23.6%. PCR MTB test of pleural fluid(+) in 34.4% of cases of tuberculous pleuritis. Histology of pleural needle biopsy – malignant in 23.4% of with 10.6% mesothelioma, pleural tuberculosis in 34.1%, other etiology in 42.5% of cases.
diagnosis ; Pleural Effusion ; Diagnostic Techniques, Respiratory System

This paper briefly introduces the working procedure of in vitro diagnostic products (IVD) industrial standards, and elaborates the importance of professional standards for production and supervision. Based on the analysis of working progress during the past 10 years, some problems and countermeasures on project setting, participation, standard material, personnel training, work cycle are put forward, which are helpful for the future development of the IVD.
Diagnostic Techniques and Procedures ; standards ; Humans ; Reference Standards

Humans ; Molecular Diagnostic Techniques ; standards ; Quality Control

Exacerbations of asthma is major causes of morbidity, mortality, and health-care costs. Over the decade, studies using new molecular diagnostic techniques have established that respiratory viruses are a major cause of exacerbations of asthma. The most prevalent viruses detected during exacerbations are the rhinovirus. Despite the burden of disease associated with exacerbations, little is known about the mechanisms of virus-induced exacerbations of airway diseases. Exacerbations are associated with increased airway inflammation in patients with asthma, but many questions remain unanswered regarding the key inflammatory cells and mediators involved. Identifying the key inflammatory mediators involved in exacerbations holds the promise of developing diagnostic and prognostic markers of exacerbation. The purpose of this review is to summarize the recent change in our understanding of the clinical manifestations of rhinovirus infection than occurred as a result of the improved diagnostic sensitivity provided by the multiplex-polymerase chain reaction (PCR) assays and serotypes.
Asthma ; Humans ; Inflammation ; Molecular Diagnostic Techniques ; Rhinovirus

Biochip analytical technology shows high throughput property for multi-samples measurement, so can reduce the required amount of samples and time used for determination. The technology quickly developed in recent years and has been applied in medical diagnosis and other analytical areas including gene chip, protein chip, lab-on-a-chip, tissue microarray, cell microarray, carbohydrate microarray and so on. This paper overviewed the current development of biochip technology, and explored the perspective of its application.
Diagnostic Techniques and Procedures ; Microchip Analytical Procedures ; methods

Laboratory medicine is a specialized division that supports physicians in the care of patients by providing rapid and accurate in vitro diagnostic tests. Standardization of every component of a specific test is essential for producing accurate results. The Clinical and Laboratory Standards Institute (CLSI) was founded to develop a formal consensus process for standardization in 1968, and has been publishing standards and guidelines covering all aspects of clinical, research, and other laboratory work. CLSI guidelines are widely used around the world for standardization. The CLSI antimicrobial susceptibility testing subcommittee (AST SC) consists of 6 standing and many ad hoc working groups. Members of the AST SC review submitted proposals and suggestions, decide on approving these submissions in face-to-face meetings held twice a year, and revise CLSI documents accordingly. As these face-to-face meetings are open to anyone who registers to attend, I strongly encourage the members of our Society to attend and actively participate in document development.
Consensus ; Diagnostic Tests, Routine ; Humans ; In Vitro Techniques