1.Establishment of a guinea pig model of dermatophytosis caused by Trichophyton verrucosum
Chinese Journal of Dermatology 2012;45(8):582-583
Objective To establish a guinea pig model of dermatophytosis caused by Trichophyton verrucosum.Methods Thirty-two guinea pigs were randomly and equally divided into four groups to remain untreated (group 1 ),receive depilation and skin abrasion in the back (group 2 and 4),or only depilation (group 3).The back was smeared with a suspension (100 μl) of Trichophyton verrucosum in group 1,2 and 3,and with that of Trichophyton mentagrophytes in group 4.Direct microscopy,fungal culture and histopathology were performed to validate the infection of guinea pigs by Trichophyton.Results Direct microscopy was persistently negative in group 1,turned to be positive from the 7th to 9th day in group 2,3,and 4.Colonies began to appear 7 days after the inoculation of scrapings onto Sabouraud's dextrose agar (SDA) in group 2 and 3,and were observed at all the inoculated sites 2 weeks after the inoculation,with a morphological similarity to Trichophyton verrucosum before inoculation into guinea pigs.Hematoxylin and eosin staining revealed strongly refractive hyphae and spores in stratum corneum epidermidis and infundibular regions of some hair follicles in group 2 and 3.Conclusion A guinea pig model of dermatophytosis caused by Trichophyton verrucosum is successfully constructed.
2.Expression of p21and Cyclin-dependent Kinase 2 in Bowen's Disease
Deyuan YOU ; Limin CAI ; Haiyan YOU
Chinese Journal of Dermatology 2003;0(11):-
Objective To explore the expression and implication of p21and cyclin-dependent kinase2(CDK2)in Bowen's disease.Methods The expression and distribution of p21and CDK2on the skin le-sion and normal skin were detected by using immunohistochemical technique streptavidin-peroxidase(SP)in28cases of Bowen's disease and10normal controls.Results p21and CDK2were significantly highly ex-pressed in Bowen' s disease and were not expressed in normal skins.Positive staining was found in22/28(78.6%)of Bowen' s disease for p21and26/28(92.9%)for CDK2.There was a correlation between the expression of p21and CDK2(r =0.84,P
3.Immunohistochemical Study on Bcl-2 and PCNA in Mixed Tumor of Skin
Deyuan YOU ; Yandong WANG ; Haiyan YOU
Chinese Journal of Dermatology 1994;0(05):-
Objective To investigate the expression of bcl-2 and PCNA in mixed tumor of skin. Methods Bcl-2 and PCNA expression in 12 cases of mixed tumor of skin were studied using immunohistochemical S-P method and double-staining. Results Positive reactions of bcl-2 and PCNA were observed in all cases. PCNA staining was positive in tumor cells lining cystic and duct-like structures in tumor nests, but bcl-2 staining was positive in epitheloid tumor cells. Most tumor cells with bcl-2 were PCNA negative and vice versa. Conclusion Aberrant expression of bcl-2 and PCNA in mixed tumor of skin may play a role in tumorigenesis.