Objective　This was a follow-up study of suicidal patients to assess the influence over time of different risk factors.Method　A cohort of 73 patients admitted to a psychiatric department after a suicide attempt was followed-up for 5 years.Results　In total,61.6% reattemped,15.1% of which had committed suicide during the follow-up.The rist factors for suicide included history of family psychiatric or suicide,and a poor social support,et al.Conclusion　The results suggest a high repetition rate of suicide attempters in the psychotic patients who had previous suicidal behaviour and them positive family history.The preventive strategy to suicide in patients,such as set up good social network and should pay mroe attention to psyciologic rehabilitation period after discharge.

Objective To analyze the features of drowning victim s with psychiatric disorders to support further investigation on such cases. Methods O ne hundred and five archives of drowning victim s with psychiatric disorders from January 2010 to June 2014 in H arbor Public Security Subbureau, Shanghai Public Security B ureau were reviewed. The decedents’ general inform ation, autopsy findings, case inves-tigation data and disease histories were statistically analyzed. Results O f the 105 cases, 104 were sui-cides. Most decedents were poorly educated. Most suicides occurred in warm clim ate, and the corpses were usually found within 2 days. Most decedents had no suicide history and notes, but had abnorm al m ental or behavior changes before suicide. D epression, depression status and schizophrenia were the m ain types of psychiatric disorders, and only 9 decedents had received regular therapies before their deaths. Conclusion Mental disorder is a high risk factor in the drowning victim s. G uardians should be aware of psychotic abnorm al behaviors, especially during warm clim ate seasons.

The present report is concerned with the surface structure of the metacercaria of Pa-ragonimus iloktsuenensis as visualized with the scanning electron microscopy. The meta-cercariae were obtained from the liver of tne Sesarma dehaani collected in Yingkou county, Liaoning Province.The tegumental spines of the metacercaria are single-pointed and densely distributed over the entire body and are slightly different in size and shape in different parts of the body. On the forebody of the ventral surface, a vertical ridgeline can be seen in the middle of the spines.In addition to the short spines, there are two circles of non-ciliated papillae on both lips of the oral and the ventral suckers. Around the oral sucker, there are 12 papillae on the outer circle and 6 on the inner circle; around the ventral sucker, there are 6 papillae on both the outer and inner circles. No sensory papillae were found around the ventral sucker. On each side of the anterior part of the ventral side, 8 to 10 pairs of papillae are arranged in two rows.

AIM:Long bone growth plate injury induced by wound and infection may cause limb reduction or angular deformity. Tissue engineering provides a promising treatment of growth plate injuries. In this study,we investigated the feasibility of establishing tissue-engineered growth-plate by allograft demineralized bone matrix(DBM) co-cultured with rabbit iliac growth-plate cells. METHODS:The experiment was performed at Department of Orthopaedics,Fourth Military Medical University of Chinese PLA from June 2005 to June 2006. ①Two 3-week-old New Zealand rabbits irrespective of gender(clean grade,2.0-2.5 kg) were selected. Growth plate cells were harvested from iliac crest epiphyseal cartilage of the rabbits by dissection and digestion with type Ⅱ collagenase. The third passage cells cultured were collected and incubated on allograft demineralized bone matrix. ② Histology,immunohistochemical staining and electronic scanning microscope(SEM) examinations were performed to observe cell growth on DBM 24 hours,7,14 and 21 days after culture. RESULTS:①Growth plate chondrocytes exhibited polygonal in monolayer culture,and immunohistochemical staining for type Ⅱ collagen was positive. ②SEM examination showed that twenty-four hours after coculture,the cells adhered to DBM scaffolds;Seven days after culture,the growth-plate chondrocytes rapidly proliferated and began to secret extracellular matrix;cells covered the whole scaffold and became overlapped on the 21st day. ③HE staining showed after 14 days of culture,growth plate cells adhered DBM scaffold in spherical shape with abundant cytoplasm. CONCLUSION:Tissue-engineered growth-plate is successfully constructed by allograft mineralized bone matrix co-cultured with rabbit iliac growth-plate cells.

Objective To retrospeetivley explore the expanded radicM resectable range and improved Surgical approach of the progressive pancreatic head cancer with metastasis of portal vein(PV) and liver,and try to improve the resectable rate.survival rate and quality of life.Methods The patients witll late pancreatic cancer sis of partial PV resection and 8 cases of left leaf or right leaf sectional hepatectomy partial were involved in 27 cases,reconstructed gastrointestinal tract with improved Whipple interposed Y-type jejunostomy and PV.27 cases were randomly divided into intervention chemotherapy of treatment groups(n=13) and control groups(n=14).Results Severe complications or dead cases were not found in 27 cases after operation.The 2-,3-,5-years survival rates were 61.5%,38.4%,23.0%,and 42.8%,21.4%,14.3% in treatment group and control group,respectively.The survival rate was statistically different(P<0.05).Conclusions The expanded radical pancreatoduodenectomy and its improved surgical approach can improve not only the survival rate but also the quality of life.