Objective To develop an image analyzing procedure for automatic localization of facial features on infrared images.Methods An unsupervised local and global features extraction method was adopted for the localization of facial features of frontal view face image.First,a threshold was used to segment the image into foreground and background,and the nose was localized by considering the symmetry of the face.Second,Harris operator was adopted to detect interest points in a rectangular area covering all the facial features,and then local maximum of the interest points were detected.And finally,K-means clustering method was used to cluster the points and obtain the facial features localization.Results The experimental result of 100 images demonstrated that the procedure could automatically localize eyes,nose,mouth,and distinguish the feature areas.Conclusion The proposed infrared image analyzing procedure based on Harris operator and K-means clustering can be used to locate facial features on infrared image more rapidly and reliablely.

Application of lymphatic tracer has greatly facilitated lymphadenectomy of tumor resection.As a novel lymphatic tracer,activated carbon nanoparticles suspension has high lymph taxis and can stain lymph nodes black as indicators.Thus,carbon nanoparticles can help to track tumor lymphadenectomy,modify the efficiency of lymphadenectomy and improve the prognosis of patients.Now it has been widely applied in the area of surgical treatment of tumors,such as gallbladder cancer,pancreatic cancer,liver cancer,etc.

Objective To analyze the expression and regulation of HES1 in sperms with low motility.Methods Thirty semen samples from asthenospermia patients and 20 semen samples from healthy and fertile adults were collected,total RNAs were extracted to produce cDNAs probes.Hybridization with Phalanx OneArray~(TM) containing 30 968 probes was carried out after the labeled cDNAs were purified by PCR product purification kit.Realtime RT-PCR was used to analyze the expression of hsa-miR-487a and hsa-miR-193b;the expression of the target genes of hsa-miR-487a and hsa-miR-193b were searched from gene-expression profiles in asthenospermia patients' sperms.Results The expression level of HES1 in low motility sperms was up-regulated.The expression level of hsamiR-193b in low motility sperms was 2.19 times higher than that in high motility sperms,hsa-miR-487a was 0.43% of that in high motility sperms.Conclusion The expression level of HES1 in low motility sperms was up-regulated.Hsa-miR-487a and hsa-miR-193b may affect the expression of HES1 and so regulate sperm motility.

OBJECTIVEThis study aims to investigate the dental caries status of 12-year-old children (born in 1998) in Sichuan province in 2010 and 2011, monitor the tendency of dental caries, and update available information and scientific basis for oral health care program.

METHODSThe oral health of 733 twelve-year-old children was surveyed for two consecutive years according to the methods proposed by the World Health Organization. The children were selected using multi-stage, stratified, randomized sampling. The collected data were analyzed using SPSS 17.0 software.

RESULTSCaries prevalence, DMFT, and DMFS in 12-year-old children were 34.8%, 0.93, and 1.09, respectively, and increased to 44.3%, 1.08, and 1.40 in the following year. The eruption of permanent teeth among females was higher than that of males (P < 0.05). The significant caries index was 2.54 in 2010 and 2.90 in 2011. F/D+F were 4.2% and 6.5% separately. Annual incidence of dental caries was 9.6%.

CONCLUSIONCaries prevalence in the permanent teeth of 12-year-olds is high and worsens as the children age. Despite the high incidence of dental caries, the filling rate is low, hence the need for immediate preventive measures.

Child ; China ; epidemiology ; DMF Index ; Dental Caries ; epidemiology ; Female ; Humans ; Male ; Oral Health ; Prevalence ; Prospective Studies ; Tooth Eruption

Objective:To assess whether ART sealants could be applied on children in school environment in China, and to evaluate long term effects of this treatments. Methods:The study was conducted in Deyang, Sichuan Province. Four dentists placed a total of 191 fissure sealants in 140 children in four secondary schools. Standard instruments and procedures for ART were used. The material used was a high-strength glass-ionomer (Ketac-Molara, ESPE). The treatments were evaluated annually by the same dentist who was not involved in the treatment using explorers and mouth-mirrors. Results:The cumulative 1-year, 2-year, 4-year and 6-year complete survival rates of the sealants were 75.4%,62.5%,50.0%,34.6% and the partial or complete retained survival rates were 89.6%, 78.8%, 68.5% and 57.9%. No incidence of new caries was occurred 1 year after ART sealants. The incidence of recurrent caries was very low after 2 and 3 years, which were 1.6% and 2.8% respectively. But there was a high increase of caries 4 years after sealants application. The prevalence of caries were 8.5%,16.1%,15.0% respectively over 4, 5 and 6 years after ART sealants application. Conclusion:The ART approach for preventing teeth caries in school children is effective and acceptable. The 3-year survival rates of the sealant are high with low prevalence of new caries. Six years after sealants application, the result is acceptable though there is a drop of survival rate. New caries is occurred with a highly increased prevalence three years after sealants missing without re-sealant. ART sealant is a simple, acceptable technique and is suitable for widespread application in China.

Objective:To investigate the application value of fusion indocyanine green fluorescence imagine (FIGFI) in laparoscopic common bile duct reexploration.Methods:The clinical data of 65 patients who underwent laparoscopic common bile duct reexploration at Henan Province People′s Hospital from Jan 2015 to Dec 2019 were collected. According to the operational manner, the patients were divided into the conventional laparoscopic group (control group, 35 patients) and the FIGFI laparoscopic group (study group, 30 patients). The intraoperative and postoperative data were analyzed.Results:Operation time, time to identify extrahepatic bile duct, intra operative blood loss, and conversion to open surgery were (195.7±9.2) min vs (147.2±9.3) min, (39.3±3.7) min vs (21.8±1.8) min, (203.2±34.6) ml vs (108.9±32.1) ml, 8 vs 1, between the control group and the study group, respectively ( P<0.05). Postoperative cholangitis, bile leakage, intra abdominal infection, pancreatitis, and hospital stay were 11vs 2, 9 vs 2, 8 vs 1, 8 vs 1, (13.5±0.9) d vs (7.4±0.9) d, between the control group and the study group, respectively ( P<0.05). There was no statistically difference in case of residual stones (5 vs 3) and gastrointestinal fistula (3 vs 1) between the control group and the study group, respectively ( P>0.05). Conclusions:FIGFI provides real-time visualization of the extrahepatic biliary tract while doing laparoscopic common bile duct reexploration.

Objective To investigate the requirment of family rehabilitation training and provide instructions and references to guide parents practising family rehabilitation training for cerebral palsy (CP) children in Shanghai.Methods300 CP children were surveyed with the Requirement Questionnaire of Family Guidance Training Program of Rehabilitation for Cerebral Palsy Children. Then 100 children out of them were selected randomly and a visiting training and guidance of rehabilitation in family was afforded.ResultsAfter providing training and guidance of rehabilitation in family, the requirement of family rehabilitation increased from 41.5% to 92.7% ( P<0.01) and the duration and the times of training were also increased ( P<0.01).ConclusionThe families having CP children in Shanghai don't understand fully about the family rehabilitation. The visiting training and guidance program will be helpful for CP children having complete rehabilitation and coming back to society.

BACKGROUND: Among multiple signals which affect the function of melanocyte, nitrogen monoxide (NO) has been thought as an important signal molecule. Emodin hypo-acid, effective component of rhubarb, can affect proliferation of melanocyte and regulate activity of tyrosinase in cells. OBJECTIVE: To study the effect of emodin hypo-acid on expression of nitricoxide synthase (NOS) of melanocyte in skin of guinea pig and definite the effective concentrations and mechanism of melanocyte in synthesizing melanin in living skin.DESIGN: Completely randomized grouping design and controlled study.SETTING: Department of Dermatology, Affiliated Hospital of Luzhou Medical College.MATERIALS: The experiment was completed at the Infection Laboratory of Affiliated Hospital of Luzhou Medical College and Laboratory of Histology and Embryology of Luzhou Medical College from January to June 2004. A total of 24 adult healthy male guinea pigs were randomly divided into 6 groups: control group, 2, 5, 10, 20 and 40 mg/L emodin hypo-acid groups with 4 in each group.METHODS: ① Emodin hypo-acid was diluted with dimethyl-sulfoxide into 2, 5, 10, 20 and 40 mg/L, then guinea pigs in emodin hypo-acid groups were injected subcutaneously with relevant dosages of emodin hypoacid which was provided by National Institute for the Control of Pharmaceutical and Biological Products into local skins of haunch and back, and the injected volume was 1 mL. Twenty-four hours later, 1 mL emodin hypo-acid of the same concentration was injected once again into one of the places which was injected before. Skin samples were selected after 48 hours and routine paraffin section was made. Skins which were not injected with any drugs were selected from control group and emodin hypoacid groups to regard as experimental control group. ② Expression of NOS was assayed with immunohistochemical method and melanocyte was identified under high-times optic microscope to observe stain and characteristic of cytoplasm. Five sections were randomly selected from each group. Every 20 cells on each section were measured with MLAS-1000 imaging analyzer and computer processing system was used to calculate average absorbency (A) of positive immune product in melanocyte. ③ Measurement data were compared with analysis of variance, and differences among groups were compared with SNK-q.MAIN OUTCOME MEASURES: Expression of NOS in melanocyte was assayed with immunohistochemical method and results were obtained with optic microscope and imaging analyzer.RESULTS: Average A value of positive immune product in melanocyte was lower in 2, 5, 10, 20 and 40 mg/L emodin hypo-acid groups than that in experimental control group (0.126±0.118, 0.103±0.082, 0.118±0.097,0.122±0.095, 0.112±0.078, 0.196±0.066, P ＜ 0.05). There was no significant difference among emodin hypo-acid groups at various concentrations.CONCLUSION: ① Emodin hypo-acid can regulate expression of NOS in melanocyte through NO signal transduction pathway. ② Expression of NOS is not changed as the mass concentration of emodin hypo-acid varied from 2 to 40 mg/L.

AIM: To observe the effect of continuous inhaled nitric oxide (NO) on airway resistance in guinea pigs. METHODS: 36 healthy male guinea pigs were divided into control group, isoproterenol group and two inhaled NO (20?10 -6 and 60?10 -6 ) groups. Respiratory resistance (R_E) and dynamic compliance(C_ dyn )were recorded before and after evoked by histamine at different doses. RESULTS: After injections of intravenous histamine at 80,120 and 160 ?g/kg, the R_E of inhaled NO groups were apparently lower than that of control group. Compared with control group, the C_ dyn of inhaled NO groups were significantly higher after administration of histamine at 80, 120 and 160 ?g/kg. After given histamine at more than 80 ?g/kg,the R_E of inhaled NO groups were higher and the C_ dyn lower than those of isoproterenol group. CONCLUSION: Inhalation of 20?10 -6 NO and 60?10 -6 NO can inhibit the increase in airway resistance induced by higher doses (80,120 and 160 ?g/kg )of intravenous histamine, but the effect of intravenous isoproterenol seems stronger.

Objective To construct antisense survivin eukaryotic expression vector pIRES2-EGFP-SVVas and detect its expression in human malignant glioma cell line SHG-44 cells. Methods The antisense survivin eukaryotic expression vector pIRES2-EGFP-SVVas was constructed and then transfected into SHG-44 cells with lipofectamine. The expression of survivin on the cells was measured by RT-PCR and Western blotting. Results The mRNA and protein expression of survivin decreased in the transfected cells. Conclusion pIRES2-EGFP-SVVas can decrease the expression of survivin in the cells transfected with the vector.