Objective: To evaluate the effectiveness of laser therapy in the treatment of dentin hypersensitivity in China.Methods: The Cochrane reviewer's handbook was followed.Domestic published articles from 1989～2009 were selected by computer and handsearch.All these retrieved study were clinical control trials related to laser therapy in the treatment of dentin hypersensitivity.The quality of included studies was critically evaluated and data were analyzed by the RevMan 5.0.1 software.Results: A tota1 of 114 articles were selected,but only 9 were included.The results showed that: although the data of immediate efficiency of laser treating dentin hypersensitivity could be combined,significant heterogeneity was found when the other studies' data were pooled.So the data could not be merged,only the descriptive analysis could be used.The immediate efficiency for the treatment of dentine hypersensitivity using lasers was higher than the control group,the difference was statistically significant[OR=3.83,95%CI(2.86,5.12)],other period of efficacy had a trend which was superior to the control group.Conclusion: Comparing with the control group,the desensitizing of dentin hypersensitivity with laser is more effective.However,because the literature quality of this study is not high,the above conclusion needs to be verified by larger sample-size,rigorously designed,randomized controlled clinical trials.

OBJECTIVEThis study aims to investigate the dental caries status of 12-year-old children (born in 1998) in Sichuan province in 2010 and 2011, monitor the tendency of dental caries, and update available information and scientific basis for oral health care program.

METHODSThe oral health of 733 twelve-year-old children was surveyed for two consecutive years according to the methods proposed by the World Health Organization. The children were selected using multi-stage, stratified, randomized sampling. The collected data were analyzed using SPSS 17.0 software.

RESULTSCaries prevalence, DMFT, and DMFS in 12-year-old children were 34.8%, 0.93, and 1.09, respectively, and increased to 44.3%, 1.08, and 1.40 in the following year. The eruption of permanent teeth among females was higher than that of males (P < 0.05). The significant caries index was 2.54 in 2010 and 2.90 in 2011. F/D+F were 4.2% and 6.5% separately. Annual incidence of dental caries was 9.6%.

CONCLUSIONCaries prevalence in the permanent teeth of 12-year-olds is high and worsens as the children age. Despite the high incidence of dental caries, the filling rate is low, hence the need for immediate preventive measures.

Child ; China ; epidemiology ; DMF Index ; Dental Caries ; epidemiology ; Female ; Humans ; Male ; Oral Health ; Prevalence ; Prospective Studies ; Tooth Eruption

OBJECTIVETo study the anti-halitosis effect of sugar-free chewing gum through their influence on odor induced by cysteine.

METHODSTen volunteers were randomly divided into the treatment group and the untreated group; each group consisted of five volunteers. All volunteers consented to participate in a test in which breath odor was induced by cysteine. After the test, the treatment group chewed sugar-free chewing gum for 1 min, whereas the untreated group did not undergo any treatment. The effectiveness was determined by the percent reduction of H2S, CH3SH, and (CH3)2S response after the volunteers chewed gum for 1, 10, and 20 min.

RESULTSAt 1, 10, and 20 min, H2S of the treatment group was reduced by 82.68%, 92.27%, 97.47%, respectively, CH3SH was reduced by 65.49%, 73.79%, and 82.89%, respectively, and (CH3)2S was reduced by 60.45%, 73.82%, and 59.72%, respectively. The differences between the two groups at different times were significant (P < 0.05).

CONCLUSIONChewing gum can effectively inhibit cysteine-induced odor.

Chewing Gum ; Cysteine ; metabolism ; Halitosis ; therapy ; Humans

Objective:To assess whether ART sealants could be applied on children in school environment in China, and to evaluate long term effects of this treatments. Methods:The study was conducted in Deyang, Sichuan Province. Four dentists placed a total of 191 fissure sealants in 140 children in four secondary schools. Standard instruments and procedures for ART were used. The material used was a high-strength glass-ionomer (Ketac-Molara, ESPE). The treatments were evaluated annually by the same dentist who was not involved in the treatment using explorers and mouth-mirrors. Results:The cumulative 1-year, 2-year, 4-year and 6-year complete survival rates of the sealants were 75.4%,62.5%,50.0%,34.6% and the partial or complete retained survival rates were 89.6%, 78.8%, 68.5% and 57.9%. No incidence of new caries was occurred 1 year after ART sealants. The incidence of recurrent caries was very low after 2 and 3 years, which were 1.6% and 2.8% respectively. But there was a high increase of caries 4 years after sealants application. The prevalence of caries were 8.5%,16.1%,15.0% respectively over 4, 5 and 6 years after ART sealants application. Conclusion:The ART approach for preventing teeth caries in school children is effective and acceptable. The 3-year survival rates of the sealant are high with low prevalence of new caries. Six years after sealants application, the result is acceptable though there is a drop of survival rate. New caries is occurred with a highly increased prevalence three years after sealants missing without re-sealant. ART sealant is a simple, acceptable technique and is suitable for widespread application in China.

0.05 ) respectively. Before and after irradiation in the group of 100 mJ, the ratio of G 2+ /P 2+ was 1.667?0.128 and 2.135?0.156( P 0.05 ). Conclusion: The normal pulsed Nd:YAG laser irradiation alone can not increase acid residence of the enamel.

OBJECTIVEThis longitudinal study aimed to investigate the caries status of primary and permanent teeth among 6-year-old children in Sichuan Province from 2010 to 2012.

METHODSA sample that comprised 652 6-year-old children from six different elementary schools (three represented the urban areas, and the other three represented the rural areas) were examined according to a baseline, with follow-up examinations at 1, 2, and 3 years. Eruption and caries experience were re- corded using World Health Organization criteria.

RESULTSThe prevalence rates of primary tooth caries of 6-year-old children in Sichuan Province for 3 years were 74.23% (484/652), 75.61% (493/652), and 81.90% (534/652). The filling rate of the primary teeth was 5.87% (145/2,471) in 2012, with significant differences (P < 0.01) between the urban areas [10.84% (133/ 1,227)] and rural areas [0.96% (12/1,244)]. The total pit and fissure rate of the first molar was 14.11% (92/652) in 2012, with significant differences between the two areas (P < 0.01) [rural: 0.66% (2/303); urban: 25.79% (90/349)].

CONCLUSIONThe pre- valence of caries in the primary and permanent teeth of 6-year-old children was high. An increasing prevalence tendency was observed as the age increased. The prevalence of first molar caries indicated that prevention and control of dental caries should be performed as early as possible.

Child ; China ; DMF Index ; Dental Caries ; Dentition, Permanent ; Follow-Up Studies ; Humans ; Longitudinal Studies ; Molar ; Prevalence ; Tooth Eruption ; Tooth, Deciduous

Objective To study the expression and clinical significance of miR-183-5p, TβRⅠ and TβRⅡ in esophageal squamous cell carcinoma (ESCC). Methods The mRNA and protein expression of miR-183-5p, TβRⅠ and TβRⅡ were examined in ESCC cell lines ECA-109, TE-1, normal esophageal epithelial cells, tumor tissues and tumor-free tissues from 72 ESCC patients. Their clinical significance and the relationship between miR-183-5p and the latter two were analyzed. The effects of miR-183-5p on the expression of TβRⅠand TβRⅡ in ECA-109 cells and the cell functions of ECA-109 were also investigated. Results Compared with the normal esophageal epithelia cells, ESCC cell lines TE-1 and ECA-109 were statistically characterized by a high expression of miR-183-5p (all P<0.05) and low expression of TβRⅠand TβRⅡ(all P<0.05). The expression of miR-183-5p in ESCC tissues was higher than that in adjacent normal tissues, while the expressions of TβRⅠ and TβRⅡ were lower (all P< 0.05). The expression of miR-183-5p was closely related to sex, tumor differentiation, tumor staging, distant metastasis, lymphatic metastasis, and tumor location (all P<0.05). TβRⅠlevel was associated with sex, lymph node metastasis and tumor size (all P<0.05). Experimental data showed the negative correlation between the expression of miR-183-5p and TβRⅠin ESCC tissues (r= -0.521, P< 0.05). Over expression of miR-183-5p significantly inhibited the expression of TβRⅠ in ECA-109 cells (P< 0.05) and promoted the growth, invasion and metastasis of ECA-109 cells (P< 0.05). Low expression of miR-183-5p significantly promoted the expression of TβRⅠ in ECA-109 cells (P< 0.05), and suppressed the growth, invasion and metastasis of ECA-109 cells (P< 0.05). There was no significant change in the expression of TβRⅡ in the transfection experiments. Conclusion MiR-183-5p is closely related to the abnormal expression of TβRⅠ, which may exert an important role in the progression of lymphatic metastasis.

Objective To develop a kind of new machine for making animal model for pressure ulcer,and inspect its effect through experiments,in order to lay the foundation for the research of pressure ulcer experiments on animals.Methods This study developed the machine after reviewing the domestic and foreign literature,making full use of the existing experimental platform of our university.Then 55 Sprague Dawley(SD) rats were selected,after anesthesia and the skin preparation,the researchers imposed certain pressure with 70 mmHg/cm2 (1 mmHg=0.133 kPa) on the skin and muscle tissue on the inner left thigh of SD rats by using self-designed machine,pressing for 2 h,then reperfusion for 30 min,3 times a day,a total of 7 days.Results The authors developed a kind of new machine for making animal model for pressure ulcer,and successfully prepared Ⅲ phase pressure ulcers model in SD rats with success rate of 98.2％(54/55).Conclusion This machine can prepare Ⅲ phase pressure ulcers model on animals,it's easy to use and efficient,it can be used for researches in the field of prevention and cure of pressure ulcers.

Objective: To study the effect of pulsed Nd:YAG laser irradiation combined with acidulated phosphate fluorid (APF) on the acid resistance of human dental enamel. Methods: Enamel samples were prepared from 192 caries-free extracted teeth. The smooth surface and pits and fissures were treated with 12 3 g/L APF for 4 minutes after 100,150 or 200 mJ pulsed Nd:YAG laser irradiation. Then the teeth were put into 12.3 g/L of artificial caries inducing solution (acid solution) for 1,5,10 or 24 h respectively. The control teeth were treated with APF only. The amount of calcium dissolved in the solution was determined with an atomic absorption spectrophotometer and the depth of artificial caries was measured with polarized microscope. Results: The amount of calcium dissolved in the acid solution was significantly less in the groups with laser treatment than that in those without laser treatment (P

Objective To observe the difference of morphology and phagocytosis between alveolar macrophages (AMs) and pulmonary interstitial macrophages (IMs). Methods AMs were collected by lung lavage and IMs by treatment of the lung tissue with DNAse and collagenase. The two cell populations were analyzed with respect to morphology by transmission electron microscopy, and the variation of these macrophages of phagocytosis were tested by malachite green colorimetry. Results There were great differences in morphology between AMs and IMs. The phagocytosis of AMs was much stronger than that of IMs. Conclusion There is functional and morphological heterogeneity between AMs and IMs. IMs should not be regarded as the precursors to AMs.