Objective:To investigate the radiation dose and detection efficiency of artificial intelligence (AI) system for solid nodules in chest phantom with different scanning protocols.Methods:A total of 60 simulated nodules with different CT values and diameters were uniformly placed in each lung lobe and lung segment of the anthropomorphic chest phantom. GE Revolution evo CT was used to scan the chest phantom. 64 groups of images with different scanning parameters were collected at the tube voltage of 80, 100, 120, 140 kV, different noise indexes (NI 10-40 with interval 2), and other fixed parameters. The detection result of simulated nodules were recorded on AI software, and the detection rate and false detection rate were calculated, respectively, for different shapes of nodules. The mean volume CT dose index (CTDI vol) and dose length product (DLP) of each scan were recorded. Results:There were no statistically significant differences in the detection rate and false detection rate of spherical nodules and irregular nodules at different tube voltages( P > 0.05), but there were and statistically significant with different noise indices ( F=10.57, 17.77, 9.33, P < 0.001). Different tube voltages had no statistical significance for CTDI vol and DLP ( P > 0.05), while different noise indices had statistical significance for CTDI vol and DLP ( F=59.87, 60.92, P < 0.001). The detection rates of nodules were moderately or weakly correlated with noise indices, CTDI vol and DLP ( r=0.43, 0.56, -0.58, -0.78, P<0.05), but no correlation with tube voltage ( P>0.05). Conclusions:Scanning protocol has an impact on AI detection efficiency of pulmonary nodules. Reasonable scanning parameters should be selected according to different image quality requirements in clinical practice.

BACKGROUND: Diabetic wound healing remains a major challenge due to the impaired functionality of angiogenesis by persistent hyperglycemia. Mesenchymal stem cell exosomes are appropriate candidates for regulating the formation of angiogenesis in tissue repair and regeneration. Here, we explored the effects of exosomes derived from human amniotic mesenchymal stem cell (hAMSC-Exos) on the biological activities of human umbilical vein endothelial cells (HUVECs) treated with high glucose and on diabetic wound healing and investigate lncRNAs related to angiogenesis in hAMSC-Exos. METHODS: hAMSCs and hAMSC-Exos were isolated and identified by flow cytometry or western blot. A series of functional assays such as cell counting kit-8, scratching, transwell and tube formation assays were performed to evaluate the potential effect of hAMSC-Exos on high glucose-treated HUVECs. The effect of hAMSC-Exos on diabetic wound healing were tested by measuring wound closure rates and immunohistochemical staining of CD31. Subsequently, the lncRNAs profiles in hAMSC-Exos and hAMSCs were examined to screen the lncRNAs related to angiogenesis. RESULTS: The isolated hAMSC-Exos had a size range of 30–150 nm and were positive for CD9, CD63 and CD81. The hAMSC-Exos facilitate the functional properties of high glucose-treated HUVECs including the proliferation, migration and the angiogenic activities as well as wound closure and angiogenesis in diabetic wound. hAMSC-Exos were enriched lncRNAs that related to angiogenesis, including PANTR1, H19, OIP5-AS1 and NR2F1-AS1. CONCLUSION Our findings demonstrated hAMSC-Exos facilitate diabetic wound healing by angiogenesis and contain several exosomal lncRNAs related to angiogenesis, which may represent a promising strategy for diabetic wound healing.

Objective:To investigate the effect of different additional filters on the image quality and radiation dose in abdominaldigital radiography (DR).Methods:Retrospective analysis was performed on 10 cases of abdominal DR from December 2020 to January 2021 in Fuxing Hospital, and the average mAs was calculated. Using automatic exposure control (AEC) technology, a polymethyl methacrylate (PMMA) slab of the corresponding thickness (18 cm) at the same output of above mAs was confirmed and used as the attenuator to simulate the abdomen. The phantom of CDRAD 2.0 and 17 slices of 10 mm thick PMMA plates (total thickness 18 cm) were placed on the bed. The additional filters were selected as no additional filter, 2 mmAl, 0.1 mmCu+ 1 mmAl, 0.1 mmCu+ 2 mmAl respectively. The AEC levels were selected at -2, -1, 0, 1, and 2 respectively. The images were collected and analyzed by using CDRAD 2.0 software to obtain the image quality factor (IQF inv). The incident air kinetic energy on the surface of the PPMA was measured for each exposure, and PCXMC software was used to estimate the organ dose and effective dose, and a comparative analysis was made. Results:The skin incident doses at no additional filter, 2 mmAl, 0.1 mmCu+ 1 mmAl, 0.1 mmCu+ 2 mm Al were (0.546 1±0.200 8), (0.376 2±0.133 8 ), (0.285 3±0.100 1) and (0.289 9±0.099 2) mGy, respectively. The estimated effective doses were (79.63±29.24)×10 -3, (71.05±25.56)×10 -3, (63.58±22.18)×10 -3 and (67.64±23.11)×10 -3 mSv, respectively. The gonadal doses were (0.058 1±0.020 8), (0.050 0±0.018 0), (0.044 8±0.015 6) and (0.047 7±0.016 3) mGy.The IQF inv values were 4.70±0.61, 4.80±0.84, 4.60±0.55, 4.60±0.60, respectively. There were linear correlations between the effective dose and the skin incident dose under different additional filtration, and the effective doses increased with the increase of the incident doses. The dose was lowest at the addition filtration of 0.1 mmCu+ 1 mmAl. There was no significant difference in the IQF inv between groups( P>0.05). Conclusions:The optimal additional filtration for abdominal DR was 0.1 mmCu+ 1 mmAl with the image quality meeting the requirements of clinical diagnosis, the radiation dose reduced reasonably, and the objective of the optimization of radiographic parameters achieved.

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) system is a hotspot of gene editing and gene expression research, in which CRISPR/Cas13 system provides a new direction for RNA interference and editing. In this study, we designed and synthesized the corresponding gRNAs of CRISPR/Cas13a and CRISPR/Cas13b systems in non-homologous end joining (NHEJ) pathway, such as Ku70 and Lig4, and then detected the expression of ku70 and lig4 in HEK293T cells. The CRISPR/Cas13a system could efficiently knockdown the mRNA expression of ku70 and lig4 more than 50%, and CRISPR/Cas13b system also suppressed ku70 and lig4 about 92% and 76%, respectively. Also, CRISPR/Cas13a, b systems could down-regulate Ku70 and Lig4 proteins level to 68% and 53%, respectively. The study demonstrates that the CRISPR/Cas13 system could effectively knockdown the expression of RNA and protein in HEK293T cells, providing a new strategy for gene function and regulation research.
CRISPR-Cas Systems ; DNA Ligase ATP ; genetics ; Gene Expression Regulation ; genetics ; Gene Knockdown Techniques ; HEK293 Cells ; Humans ; Ku Autoantigen ; genetics

The skin pathologic scar is a skin fibrous proliferative disease characterized by abnormal proliferation of fibroblasts and overdeposition of extracellular matrix. Unclarity of genesis and development mechanism is the main reason that restricts its diagnosis and treatment. In recent years, it has been found that microRNAs play important roles in the regulation mechanism of pathological scars. The competing endogenous RNAs (ceRNAs) have microRNA response elements which can be competitively combined with microRNAs through sponge adsorption. Through the mutual regulation of RNAs, ceRNAs regulate the expression of target gene and participate in the development of disease. Based on the ceRNA hypothesis, this paper systematically reviews the biological functions and clinical significance of ceRNAs in pathological scars of skin, and discusses the role of ceRNAs and " RNA-microRNA-RNA" regulation network in pathologic scars. The ceRNA therapy may become a new model therapy for skin scars in the future.

Objective:To establish the practice training platform of medical imaging technology conforming to the demand of society and the characteristics of vocational education, and improve the training conditions and promote the quality of higher vocational talent cultivation.Methods: To carry out investigation and expert interviews in various grade hospital; analyze vocation standard and skill training grade; plan and design the layout and function position of hardware for this training platform; and then take practice research for application of this platform.Results: The training platform of medical imaging technology has been established which includes 14 digital training rooms. Every teaching function, such as radiology registration, detection, image processing, diagnostic reporting, report printing and storage capability, could achieve and correspond to a clinical task.Conclusion:This study effectively improves medical imaging technology vocational practice conditions, and has a great significance to improve teaching quality of education and ensure quality of talents cultivation.

Objective To investigate the effect of Chinese herbal medicine combined with acupuncture and massage on the treatment of knee osteoarthritis. Methods A total of 92 patients with osteoarthritis of the knee were enrolled in our hospital from September 2015 to March 2017, and were randomly divided into study group and control group (46 cases) by single and double numbers (admission order).The control group was treated with acupuncture and massage, and the study group was treated with acupuncture and massage on the basis of external use of Chinese medicine. The two groups of patients with knee osteoarthritis were treated with continuous treatment for 6 courses, the pain degree and the change of osteoarthritis index were recorded before and after the treatment. Results There was no significant difference in NRS and WOMAC scores between the two groups before treatment. The scores of NRS and WOMAC in patients with knee osteoarthritis after treatment were significantly lower than those in the control group, the difference was statistically significant(P<0.05). Conclusion The application of Chinese medicine, acupuncture and massage in the treatment of proliferative osteoarthritis of the knee can achieve more satisfactory clinical efficacy, and is conducive to protect the quality of life.

Exosomes are nano-vesicles released by many kinds of cells. Exosomes play a significant role in cell-to-cell communication and substance transportation through direct effect of signaling molecules on the cell membrane surface, intracellular regulation of cellular content during membrane fusion, or regulation of release of various bioactive molecules. Several studies have reported that culture supernatant of stem cells has some related exosomes to take part in wound repair. The secretion of exosomes is depended on the source and the physiological and pathological condition of deriving cells. How to stimulate the stem cells to produce exosomes maximally and their clinical application are worthy to explore. In this review, we summarize the biological function and application of exosomes derived from stem cells in wound repair.

As one of the self-protection mechanism, autophagy widely exists in eukaryotic cells. It plays an important role in maintaining cells survival, update, material recycling, and tissue homeostasis. A series of researches discovered that autophagy played dual function in fibrotic diseases. The induction of autophagy can promote the degradation of collagen on one hand, on the other hand, the regulation of autophagy through microRNA, transforming growth factor β, and other factors can promote the occurrence of fibrosis. In wound healing, autophagy may participate in the pathophysiological processes of inflammation, reepithlialization, and wound remodeling. The regulation of cell autophagy may become an effective way and the new target for treatment of wound and pathological scar.