0.05),however there was marked difference in diameter( P

Objective To explore the value of biopsy of lymph nodes alongside of internal jugular vein in surgery for papillary thyroid carcinoma(PTC) patients at CN 0 stage.Methods Clinical data of 103 PTC patients with operation at CN 0 stage were retrospectively analyzed.The lymph node ratio alongside of internal jugular vein was counted,and factors,such as gender,age,tumor size,capsular foreign invasion were analyzed.Results Of all 103 cases of papillary thyroid carcinoma at CN 0 stage,32 cases had lymph node metastasis alongside of internal jugular vein(31.1％).Meanwhile 31 cases had the thyroid capsule invasion,and 21 cases had lymph node metastasis alongside of internal jugular vein(67.7％).The rest 72 cases had no the thyroid capsule invasion,and 11 cases had lymph node metastasis alongside of internal jugular vein(15.3％),the difference was statistically significance(x2 =27.85,P ＜ 0.05).Conclusion Routine biopsy of lymph nodes alongside of internal jugular vein is necessary of papillary thyroid carcinoma patients at CN 0 stage.Whether the thyroid capsule is invased which is important factors affecting lymph node metastasis alongside of internal jugular vein.

The intramedullary expand self-locking nail(IESN) is a new type of solid small diameter nail consisting of the dynamic and the static types, designed on the basis of the CAIN. The mechanical experiments showed that its stiffness to resist the bending and twisting momentum was simillar to the Russell -Taylor's nail. The static nail was stable enough to prevent axial displacements of the fractured segments. The main characteristics of the IESN are as follows: 1) It is being able to be inserted without reaming the medullary cavity and without X- ray guidance; 2) a higher limit value to resist fatique and 3) no stress concentration on IESN.

10% was found in 5 patients (16%). Mesothelial hyperplasia was found to be significantly higher in patients with PTE than that in the control group (P

Objective In the present study we examined the roles of three proinflammatory cytokines, i.e. tumor necrosis factor-?(TNF-?), interleukin 1?(IL-1?), and IL-8 in the early phase of APTE, and analyze the impact of these cytokines on the systemic circulation. Methods APTE was induced in 7 anesthetized dogs by injecting the thrombus of own blood into the right jugular vein. Seven animals receiving only warm sterile saline served as controls. Arterial serum samples were collected regularly (0h, 0.5h, 1h, 2h, 4h, 6h) and cytokine levels could be measured by radio-immunity assay. Results Introduction of clots into the pulmonary circulation caused significant increase in the mean pulmonary arterial pressure(from 99.1?5.7 to 66.8?15.3mmHg, P

The occurrence of iliac vein compression syndrome(IVCS) has the anatomic factor.IVCS has no specific symptoms and signs.The diagnosis of IVCS is mainly made by venography, intravascular pressure measurement, intravascular ultrasound, Doppler ultrasound,magnetic resonance venography, and CT.Before the occurrence of acute iliofemoral thrombosis,the treatment of IVCS is conservative therapy.The purpose of surgical intervention is to resolve the obstruction and keep the blood flow. The surgery of occluded iliac veins secondary to IVCS is now to be replaced by endovascular reconstruction. IVCS can be treated correctly before the occurrence of iliofemoral thrombosis and its sequelae can be reduced greatly, if the diagnosis of IVCS can be made as early as possible,and the degree of the stenosis of the iliac vein,the characteristics of its hemorheology and hemodynamics can be understood in time.

Objective To explore the significance of carbon nanoparticles in routine central lymph node dissection of papillary thyroid microcarcinoma (PTMC).Methods 272 cases of PTMC admitted from 2013 to 2015 were retrospectively analyzed and they were divided into two groups,the experimental group who were given carbon nanoparticles during surgery (136 cases) and the control group (136 cases) without carbon nanoparticles.The total number of central lymph nodes,number of transfer,and the black dye lymph node number and transfer number in the experimental group were recorded.The total number of lymph nodes,and number of transfer in the control group were recorded.The metastasis rate of the two groups were analyzed.The number of parathyroid mistakenly cut and hypocalcemia cases of the two groups were counted.Parathyroid function was observed by determination of Ca2+ and PTH in blood.The incidence of recurrent laryngeal nerve (RLN) injury of the two groups was compared.Results The total number of central lymph nodes was 1216 and the transfer number was 481 in the experimental group,higher than those of the control group.The positive lymph node rate of the two groups was 39.6％ and 25.9％ respectively,and the difference was statistically significant.The mumber of patients with central lymph node metastasis was 63 and 42 respectively for the experimental group and the control group,and the transfer rate was 46.3％ and 30.8％ respectively.The difference was statistically significant.The rate of parathyroid mistakenly cut was 2.6％(7/261) and 14.7％ (28/193) respectively for the experimental group and the control group,and the difference was statistically significant.The difference of Ca2+ and PTH value at 3h,6h,and 12h after surgery between the two group was statistically significant.No RLN injury occured.5 cases in the experimental and 8 cases in the control group had temporary RLN injury.The difference was not statistically significant.Conclusions The application of carbon nanoparticles in PTMC surgery can help to improve the thoroughness of central lymph node dissection and to protect parathyroid function.However,its benefits to protect the recurrent laryngeal is uncertain.

Objective To investigate mRNA and protein expressions of TGF-β1 and their signifi-cance in injured spinal cord after transplantation of activated microglia. Methods Wistar rat model of spinal cord injury was made by using modified Allen's method. Then, the activated microglia was trans-planted into spinal cord injury models tu detect mRNA and protein expressions of TGF-β1 in the spinal curd. Results The mRNA and protein expressions of TGF-β1 in control group were significantly lower than that in test group (P<0.05). Conclusion Transplantation of activated microglia to the impaired spinal cord in rats can increase the mRNA and protein expressions of TGF-β1. The up-regulated expres-sion of TGF-β1 in spinal cord may play a role in promoting the recovery of spinal cord, when the activated microglia may be the main saurce of TGF-β1.

AIM: To observe the effects of local transfection of vascular endothelial growth factor 165 (VEGF165) gene on inhibiting intimal hyperplasia and restenosis of artery in rabbits after operation injury, and its possible mechanisms. METHODS: Microsurgery injury was used to establish the intimal injury model of right external iliac artery in rabbits. 105 male New Zealand rabbits were randomly divided into 3 groups (35 rabbits in each group). Group A was physiological saline control group, group B was pBudCE4.1-transfected group, group C was pBudCE4.1/VEGF165-transfected group. The physiological saline, pBudCE4.1 and pBudCE4.1/VEGF165 transfection solutions were injected into injured vessel walls of above-mentioned groups. The injured vascular specimen was harvested for pathologic examination, electric microscope observation, RT-PCR examining and immunohistochemical staining. RESULTS: Rabbit intimal thickness and area of vessel walls in group C at every time point after operation were significantly less than those in group A and group B (P

Objective To investigate the protective effect of curcumin on apoptosis of human umbilical vein endothelial cells (HUVECs) induced by oxidized low density lipoprotein (ox-LDL) and its possible mechanism.Methods Cultivated HUVECs were divided into six groups: control group, ox-LDL group, ox-LDL plus endoplasmic reticulum stress(ERS) inhibitor PBA group,curcumin group, ox-LDL plus curcumin group,ox-LDL plus curcumin plus PI3K inhibitor LY294002 group.Cell viabilities were evaluated by CCK-8 assays.The proportions of apoptotic cells were assessed by flow cytometry.The translocation of activating transcription factor 6 (ATF6) observed by laser confocal microscopy.Western blot was used to determine the expression of the ERS associated proteins:glucose-regulated protein 78(GRP78), protein kinase-like ER kinase(PERK), inositol-requiring kinase1(IRE-1) and the related pathways protein: LOX-1, AKT and phophorylated AKT.Results Compared with control group,increasedthe proportions of apoptotic cells(P<0.01),enhanced the expressions of ERS related proteins(P<0.01),promoted the transfer of ATF6 into the nucleus,as well as increased the expression of LOX-1(P<0.01)and decreased the expression of p-AKT(P<0.01) in the ox-LDL group;Compared with ox-LDL group,PBA inhibited ox-LDL-induced HUVECs apoptosis(P<0.01),curcumin inhibited ox-LDL-induced the expression of ERS associated protein and LOX-1(P<0.01), the nuclear translocation of ATF6, the apoptosis of HUVECs (P<0.01), and it also increased ox-LDL-induced down-regulation of p-AKT expression (P<0.01);LY294002 partially attenuated the inhibitory effect of curcumin on ERstress-related protein expression induced by ox-LDL(P<0.05).ConclusionsCurcumin can reduce ox-LDL induced apoptosis of HUVECs, its mechanism may be through the inhibition of LOX-1 expression and activation of AKT pathway to reduce ERS in cell.