Aims: Aloe vera (Aloe barbadensis Miller) has been cultured in Bali, Indonesia since 2006. Its cultivation area is 170 haincluding 5 regencies i.e. Buleleng, Karangasem, Bangli, Badung and Gianyar. This study was conducted to isolate andidentify the Streptomyces sp. that potentially can be used to inhibit the growth of F. oxysporum and control the leaf rotdisease on aloe vera with and to study the structural responses of F. oxysporum to the treatment of Streptomycesculture filtrate.Methodology and results: Samples were collected from Serokadan, Kerobokan, and Saba, Bali, Indonesia. The isolateof Streptomyces sp. that resulted in the highest antifungal activity was further observed on its morphological andultrastructure characteristics using SEM and TEM. Identification was done by using 16S rRNA sequencing techniques. Agreenhouse experiment was conducted to evaluate the effectiveness of the filtrate of Streptomyces sp. to control the leafrot disease on aloe vera. The Streptomyces GYRRK was identified to be S. thermocarboxydus and the filtrate inhibitedthe growth of F. oxysporum by damaging cell wall and plasma membrane of macro conidia cell, micro conidia, andhypha. Treatment with the filtrate of S. thermocarboxydus with four sprays (one spray equal to 0.5 mL) over inoculatedleaves of aloe vera reduced the leaf rot disease by 68%.Conclusion, significance and impact of study: This result suggests that filtrate of S. thermocarboxydus potentiallycan be used as an alternative control agent against leaf rot disease on aloe vera in Bali.