1.Electron Microscopic Observation of Experimentally Induced Comedones in Rabbit.
Korean Journal of Dermatology 1987;25(4):492-499
Accurnulation of keratinized cells within the infundibulum of pilosebaceous canal is important in the pathogenesis of acne. Light and electron microscopic study of experimentally induced comedones, from the rabbit's external ear canal, was performed to define the earIy morphologic changes within and around the epithelial lining of the comedones. In light microscopic observation, early comedone was composed of loose cohesive horny cells and late comedone was composed of a mixture of loose and tight cohcsive horny cells. Cohesion between horny cells occured, in eIectron microscopic study, in two different ways: initially, by the persistence of desmosomes; later, and to a lesser extent, by tight junctions, which tightly bound the horny cells together. Multiple lipid droplets within the horny ceIls and a gradual decrease in the numbrane coating granules were observed.
Acne Vulgaris
;
Desmosomes
;
Ear Canal
;
Tight Junctions
2.Sarcomatoid Renal Cell Carcinoma; Special Reference to its Distinction from Carcinosarcoma.
Kee Taek JANG ; Yeon Mee KIM ; Je Geun CHI
Korean Journal of Pathology 1998;32(5):378-381
Sarcomatoid renal cell carcinoma is an uncommon tumor that has to be distinguished from renal carcinosarcoma. We have described three cases of sarcomatoid renal cell carcinoma showing different clinical and light microscopic features. An ultrastructural study of the tumor cells from the sarcomatoid area revealed frequent desmosomal junction, confirming the epithelial nature of the neoplasm. All three cases showed an aggressive clinical course and tended to invade adjacent organs or tissues. We believe that an histological and immunohistochemical examination in conjunction with an electron microscopic examination are necessary to diagnose sarcomatoid renal cell carcinoma.
Carcinoma, Renal Cell*
;
Carcinosarcoma*
;
Desmosomes
;
Microscopy, Electron
3.Ultrastructure of Langerhans Cell and Expression of HLA-DR Antigen in Normal Limbal Conjunctiva.
Myung Kyoo KO ; Shin Hwan JOO ; Joon Kiu CHOE
Journal of the Korean Ophthalmological Society 1988;29(3):283-288
We have observed the ultrastructures of Langerhans cell in the normal limbal eonjunctiva by electron microscopy and expression of HLA-DR antigen by peroxidase-antiperoxidase(PAP) method in the normal limbal conjunctiva. In electron microscope, Langerhans cell had no junctional complex like desmosome, irregular and invaginated nucleus, and characteristic Birbeck granlues in the cytoplasm. In PAP method, HLA-DR-positive cells were found in basal or suprabasal layer of conjunctival epithelium as dendritic form.
Conjunctiva*
;
Cytoplasm
;
Desmosomes
;
Epithelium
;
HLA-DR Antigens*
;
Microscopy, Electron
4.Well-Differentiated Papillary Mesothelioma of the Peritoneum: A case report .
Yi Kyeong CHUN ; Yee Jeong KIM ; Sung Ran HONG
Korean Journal of Pathology 1998;32(9):697-699
Well-differentiated papillary mesothelioma (WDPM) of the peritoneum is considered to be a distinct subtype of peritoneal mesothelioma and has mostly behaved in a benign fashion. We report a case of WDPM in a 48-year-old-woman. It was incidentally found during a hysterectomy for a uterine cervical carcinoma. Grossly, the tumor was composed of multiple peritoneal nodules, measuring up to 2 cm. Microscopically, the nodules showed well-developed papillae lined by a single layer of cuboidal mesothelial cells. Immunohistochemical staining revealed a positive reaction for cytokeratin and a negative reacion for carcinoembryonic antigen and Leu-M1. Ultrastructurally, the tumor cells showed numerous long, slender microvilli and desmosomes.
Carcinoembryonic Antigen
;
Desmosomes
;
Hysterectomy
;
Keratins
;
Mesothelioma*
;
Microvilli
;
Peritoneum*
5.Sarcomatoid Carcinoma of the Pancreas: A Case Report.
Kyung Hee KIM ; Dae Young KANG ; Min Koo LEE ; Hyeon Woong YANG ; Hyun Young HAN
Korean Journal of Pathology 2006;40(4):306-310
We report here on an unusual case of a 73-year-old Korean female with sarcomatoid carcinoma. This tumor was composed of pancreatic ductal adenocarcinoma and a malignant fibrous histiocytoma-like stroma. The CT imaging revealed a multiseptated heterogenous hypodense mass that was 15 cm in size. The mass was located in the body and tail of the pancreas, spleen and gastrosplenic area. The pathologic examination showed that the carcinomatous component was negative for vimentin, and the sarcomatous component was positive for vimentin and CD 68. The ultrastructural examination showed that both the carcinomatous and sarcomatous components had desmosomes at the cell-cell contact sites. The patient refused postoperative adjuvant chemotherapy and she died of cachexia with generalized tumor extension about 3 months later. This report presents special data that can clarify the clinicopathological features and pathogenesis of this rare neoplasm.
Adenocarcinoma
;
Aged
;
Cachexia
;
Chemotherapy, Adjuvant
;
Desmosomes
;
Female
;
Humans
;
Pancreas*
;
Pancreatic Ducts
;
Spleen
;
Vimentin
6.Two Cases of Extramammar Paget's Disease.
Duck Pyo HONG ; Eil Soo LEE ; Dong Chul KIM ; Kye Yong SONG
Korean Journal of Dermatology 1986;24(5):712-716
We have experienced two cases of extramammary Paget's disease. The first case was a 68-year-old male who showed erythematous, crusted, oozing, eczematoid patches on the scroturn, penile root and pubic area, and verrucous plaque on the left pubic area of 5 years' duration. The second case was a 60-year-old male who showed erythematous, oozing, hyperkeratotic plaque and nodular mass on the scrotum of 4 years' duration. In electron microscopic findings of the first case, the Paget's cells was suggested to be formed by pleuripotential germinative cells; well developed microvilli, numerous electron lucent secretory granules, a few electron dense granules, apocrine type secretion and well developed desmosome.
Aged
;
Desmosomes
;
Humans
;
Male
;
Microvilli
;
Middle Aged
;
Paget Disease, Extramammary
;
Scrotum
;
Secretory Vesicles
7.A Case of Familiar Benign Chronic Pemphigus.
Nack In KIM ; Choon Bong LEE ; Moo HYUNG ; Choong Rim HAW
Korean Journal of Dermatology 1987;25(5):716-721
A 25-year-old man with familial benign chronic pemphigus presented with a one-year history of a localized pruritic recurrent eruption on his perianal area, Physical examination showed moist, macerated, fissured and scaly patches on erythematous base, A biopsy specimen showed extensive intraepidermal separation containing acantholytic cells. Electron microscopic observation showed widened intercellular space, detachment of the tonofilaments from the desmosomes, and subsequent concentration of the tono filaments around the nucleus. Microvilli were elorigated, thinned and branehed. 1)esmosomes were reduced in number. This case is unique in its clinical location, showing no family tendency.
Adult
;
Biopsy
;
Desmosomes
;
Extracellular Space
;
Humans
;
Intermediate Filaments
;
Microvilli
;
Pemphigus, Benign Familial*
;
Physical Examination
8.Matrix Metalloproteinases and Autoimmune Bulbous Diseases: Expression of MMP-2, -3, and -9 in Bullous Pemphigoid, Pemphigus Vulgaris, and Pemphigus Foliaceus.
Ji Sun CHUN ; Sook Jung YUN ; Seong Jin KIM ; Seung Chul LEE ; Young Ho WON ; Jee Bum LEE
Korean Journal of Dermatology 2009;47(11):1261-1266
BACKGROUND: Matrix metalloproteinases (MMPs) participate in extracellular matrix degradation and may play an important role in basal membrane damage in many dermatologic diseases. Recent studies implicated the importance of MMP-9 in the pathogenesis of bulla formation of bullous pemphigoid (BP). Various autoimmune bullous diseases are strongly associated with desmosome or hemidesmosome pathologies, and show an increased level of lesional MMP and exposed autoantigens from these structures. OBJECTIVE: This study evaluated the level of MMP-2, -3, and -9 in three types of autoimmune bullous disease [BP, pemphigus vulgaris (PV), pemphigus foliaceus (PF)] with the aim of investigating the role of MMPs in the pathogenesis of autoimmune bullous diseases. METHODS: Sample specimens were obtained from skin lesions of patients with BP (n=12), PV (n=10), and PF (n=12), and from normal controls (n=8). The immunohistochemical expression of MMP-2, -3, and -9 was analyzed and serum levels of MMP-2, -3, and -9 were measured by enzyme-linked immunosorbant assay (ELISA). The results were analyzed with reference to graded levels of clinical severity. RESULTS: Expression of dermal MMP-2, -3, and -9 were increased in BP, PV, and PF (p=0.036, 0.022, and 0.015, respectively). However, decreased expression of the three MMPs in the epidermis of skin lesions may have resulted from epidermal destruction. ELISA-determined serum levels of MMP-2, -3, and -9 increased in BP, PV and PF. Interestingly, MMP-2 was significantly increased in the sera of BP patients (p=0.015), consistent with the previous studies concerning the role of gelatinase (MMP-2 and -9) in the pathogenesis of BP. In BP patients, clinical severity was proportional to increased levels of MMP-2 in both skin lesions and and sera. CONCLUSION: The increased expression of MMP-2, -3, and -9 in skin lesions and sera may reflect the involvement of these enzymes in the mechanism of bulla formation in autoimmune bullous diseases including BP. In addition, expression of MMP and clinical severity may be closely connected.
Autoantigens
;
Blister
;
Desmosomes
;
Epidermis
;
Extracellular Matrix
;
Gelatinases
;
Hemidesmosomes
;
Humans
;
Matrix Metalloproteinases
;
Membranes
;
Pemphigoid, Bullous
;
Pemphigus
;
Skin
9.The Mechanism of Moisturization by Glycerin and Propylene Glycol: Their Effects on the Intercorneocyte Lipid and Desmosome.
Sang Min HWANG ; Sang Hoon PARK ; Sung Ku AHN ; Seung Hun LEE
Korean Journal of Dermatology 1999;37(12):1715-1723
BACKGROUND: Moisturizers induce skin hydration and then increase flexibility and elasticity, making the skin soft and smooth, and protecting it against environmental stimuli. OBJECTIVE: The aim of this work was to study the role of intercorneocyte lipid layers and desmosomes in the mechanism of moisturization. METHODS: Transepidermal water loss (TEWL) and capacitance were measured and the morphologic changes of the intercorneocyte lipid layers and desmosomes with electron microscopy, using ruthenium tetroxide (RuO4) postfixation, following the application of glycerin, propylene glycol, and a mixture of glycerin and propylene glycol for a 2 hour period to the epidermis of hairless mice were measured. RESULTS: 1. The TEWL was significantly increased in all three groups; glycerin, propylene glycol, and mixture of glycerin and propylene glycol. The increase of TEWL after the application of glycerin was maintained from the second to the forth hour after application which was statistically significant, after the application of propylene glycol it was maintained for 5 hours, and after the application of a mixture of glycerin and propylene glycol, for 6 hours. 2. The capacitance also was increased in all three experimental groups, compared to the control group. However there was no statistical significance. 3. Light microscopic findings showed no specific changes in all three groups, compared to the control group. 4. Ultrastructural observation by electron microscope, using RuO4 postfixation, showed widening of the intercorneocyte lipid layers in all three groups. In contrast to glycerin in which the results showed detachment of the desmosomes without changes in the intercorneocyte lipid layers, propylene glycol showed interruption and undulation of the intercorneocyte lipid layers and expansion of the lacunae spaces. A mixture of glycerin and propylene glycol showed interruption and undulation of the intercorneocyte lipid layers, detachment of the desmosomes, and, partial, formation of lacunae. CONCLUSION: These results suggest that the moisturizing effects of glycerin result from an increased detachment of the desmosomes and widening of the intercorneocyte lipid layers and then an increase in the water holding capacity of the stratum corneum. Propylene glycol, a chemical penetration enhancer, induce widening, interruption, and undulation of the lipid layers and expansion of the lacunae space. In the mixture of glycerin and propylene glycol, propylene glycol potentiate and continue the moisturizing effects of the glycerin.
Animals
;
Desmosomes*
;
Elasticity
;
Epidermis
;
Glycerol*
;
Mice
;
Mice, Hairless
;
Microscopy, Electron
;
Pliability
;
Propylene Glycol*
;
Ruthenium
;
Skin
10.Ultrastructural Findings of Bowenoid Papulosis of the Genitalia.
Choong Seop HAHN ; Soo Il CHUN ; Jung Bock LEE
Korean Journal of Dermatology 1982;20(3):479-487
A 41 year-old man had dark pigmented papules and plaques, 4 to 15 mm in size, on the proximal portion of the dorsum of the penile shaft and pubic area of 8 months duration. Over next several months, flesh colored, macerated papules, 2 to 4 mm in size, appeared on the left side of the scrotum Light microscopic examination from a pigmented lesion of the penile shaft showed typical findings of Bowen's disease. Ultrastructural findings included: widening of intercellular space, a decrease in the number of desmosomes with microvillous projections, aggregation of tonofilaments, an increase in the mitotic keratinocytes, an increase in the number of melanin granules, and disorganized bundles of tonofilaments surrounding chromosomes. Virus-like particles were not observed. Based on the ultrastructural findings and review of literatures, it seems to be probable that bowenoid papulosis is a clinical variant of Bowens disease.
Adult
;
Bowen's Disease
;
Desmosomes
;
Extracellular Space
;
Genitalia*
;
Humans
;
Intermediate Filaments
;
Keratinocytes
;
Melanins
;
Scrotum