OBJECTIVE: To determine the content of Sildenafil citrate illegally added in kidney-yang reinforcing Chinese medicinal tonics. METHODS: The samples were separated on ODS C18(250 mm?4.6 mm,5 ?m) with mobile phase consisted of methanol-water-acetic acid glacial-triethylamine(60∶40∶0.8∶0.3) at a flow rate of 0.8 mL?min-1.The UV detective wavelength was 290 nm and the sample size was 10 ?L. RESULTS: At a concentration range of 10～100 ?g?mL-1, Sildenafil citrate showed a good linear relation with peak area score. The mean recovery rate was 100.52%(RSD=1.20%). CONCLU-SION:The method can be used to determine Sildenafil Citrate illegally added in kidney-yang tonics.

Objective:To explore the significance of exposing the circummammary ligament under endoscopy and the effect of membrane anatomy in breast reconstruction.Methods:The case data of 49 breast cancer patients who underwent endoscopic nipple-sparing mastectomy combined with one-stage breast reconstruction with prosthesis implantation in Beijing Friendship Hospital, Capital Medical University from February 2014 to December 2021 were retrospectively analyzed, there were 44 cases of posterior pectoralis prosthesis implantation, 5 cases of anterior pectoralis prosthesis implantation. The anatomical structure of the circummammary ligament was observed under endoscopy during operation, and the annular mammary ligament was used as an anatomical marker to complete subcutaneous glandectomy and prosthesis implantation for breast reconstruction, the BREAST-Q scales were used to evaluate the postoperative effect.Results:The medial sternal ligament, sub clavicular ligament, lateral confluence ligament and triangular ligament condensation could be clearly exposed in all 49 cases. Breast reconstruction module of BREAST-Q were used to evaluate the surgery effect after breast cancer surgery, the scores of postoperative breast satisfaction, chest wall status, psychosocial status and sexual health status were 81.43±12.57, 88.39±10.61, 88.04±13.70, 74.82±15.93.Conclusion:The endoscopic technique is beneficial to expose the circummammary ligament during operation, and surgical resection and reconstruction can better restore the appearance of the breast and improve postoperative satisfaction according to the principle of membrane anatomy.

Aspergillosis ; Humans ; Mucor ; Mucormycosis/drug therapy*