Objective To explore the inhibitory effect of dexamethasone on the expression of protein kinase C(PKC) in the lung,the C-7-T-5 spinal ganglia and the corresponding spinal dorsal horns of the asthmatic guinea pigs. Methods The alterations of PKC immunoreactivity were investigated by means of immunohistochemistry and Western blot in the lung,the C-7-T-5 spinal ganglia and the corresponding spinal dorsal horns of the asthmatic guinea pigs.Results The average optic density of PKC in the lung,the C-7-T-5 spinal ganglia and the corresponding spinal dorsal horns of the asthmatic guinea pigs was much higher than that of the control group(P

Aim To explore the inhibitory effect of dexamethasone on the expression of IL-1? in the lung and the visceral sensory afferent system(C_7-T_5 spinal ganglia and the corresponding posterior horn of the spinal cord) of asthmatic guinea pigs. Methods Experimental asthma model of guinea pigs was induced by ovalbumin in vivo. The expression of IL-1? was detected by means of immunohistochemistry in the C_7-T_5 spinal ganglia and the corresponding posterior horn of the spinal cord of all guinea pigs. The expression of IL-1? protein was investigated by Western blot in the lung, C_7-T_5 spinal ganglia and the corresponding posterior horn of the spinal cord of all guinea pigs. Results The expression of IL-1? in the lung, C_7-T_5 spinal ganglia and the corresponding posterior horn of the spinal cord of the asthmatic guinea pigs was much higher than that of the control groups(P

Objective To observe the repairing effect of the acellular nerve allografts on the sciatic nerve gap of rat. Methods The acellular nerve allografts,treated by hypotonic-chemical detergent,were put on the 10 mm gap of the sciatic nerve in the rat.The action potential of the regenerated nerves was determined by the electrophysiologic method 13 weeks after operation.The morphology of the regenerated nerves was observed under light microscope and electron microscope,and the results were analyzed statistically. Results No inflammation and rejected reaction were found in the period of 13 weeks after operation in the operated and control groups.There was no significant difference in number of the regenerated nerve fibers,diameter of the axons,and the thickness of the regenerated myelinated nerve between the experimental group and control group.Conclusion The present results indicated that the acellular nerve allografts had good biocompatibility for the host rat in vivo and might as a bridge promote the regeneration of the injured sciatic nerve.;

0.05).The expression of TNF-? mRNA was significantly upregulated in the lower respiratory tract,C-7-T-5 spinal ganglia and corresponding spinal dorsal horn of the experimental asthmatic guinea pigs compared with the normal saline control group and the simple sensitized group(P

Objective The experiment was designed to investigate the effect of acellular nerve allografts on the functional recovery and reconstruction of the nerve-muscle structure of the sciatic nerve defect in rats. Methods Acellular nerve allograft was transferred into the defected rat sciatic nerve with 10mm long.The wet weight of tibialis anterior was weighed at 12 and 24 weeks postoperatively compared with control group.The conducted velocity of regenerated nerve and the effect of regenerated nerve on tibialis anterior were investigated by electrophysiologic test,and silver staining combined with AChE histochemical methods were used in the experiment separately. Results The wet weight of tibialis anterior and the conducted velocity of regenerated nerve in experimental group were similar to those in control group in 12 and 24 weeks after transplantation.The positive acetylcholinesterase(AChE)histochemical reaction was observed in the tibialis anterior at 12 weeks with deeper staining and located in the middle of tibialis anterior tidily at 24 weeks after operation.The regenerated nerve bundles and nerve terminals were found to grow into the motor end-plate of the tibialis anterior in silver staining combined with AChE staining in experiment group.Electromyogram showed that the regenerated nerve has innervated tibialis anterior already.Conclusion The results indicated that extracted nerve allografts as a bridge can promote the motor functional recovery and reconstruction of the nerve-muscle structure of the defected rat sciatic nerve.

Advances in biological and medical technologies have been providing us explosive volumes of biological and physiological data, such as medical images, electroencephalography, genomic and protein sequences. Learning from these data facilitates the understanding of human health and disease. Developed from artificial neural networks, deep learning-based algorithms show great promise in extracting features and learning patterns from complex data. The aim of this paper is to provide an overview of deep learning techniques and some of the state-of-the-art applications in the biomedical field. We first introduce the development of artificial neural network and deep learning. We then describe two main components of deep learning, i.e., deep learning architectures and model optimization. Subsequently, some examples are demonstrated for deep learning applications, including medical image classification, genomic sequence analysis, as well as protein structure classification and prediction. Finally, we offer our perspectives for the future directions in the field of deep learning.
Algorithms ; Computational Biology ; methods ; Diagnostic Imaging ; Genomics ; methods ; Humans ; Image Interpretation, Computer-Assisted ; methods ; Machine Learning ; Neural Networks (Computer) ; Protein Structure, Secondary ; Proteins ; metabolism