BACKGROUND: Continuous oxygen inhalation of high concentration may improve antioxidase and simultaneously injure the lung in rats.OBJECTIVE: To observe the effect of intermittent high concentration oxygen on antioxidase activity and pulmonary tissue in rats.DESIGN: Complete randomized control experiment.SETTING: Department of Geriatric Cardiology and Department of Biochemistry, General Hospital of Chinese PLA. MATERIALS: The experiment was performed in Department of Hyperbaric Oxygen of Navy General Hospital and Department of Biochemistry of General Hospital of Chinese PLA from January 2002 to May 2002, in which, 10 healthy Wister rats were employed, mass weighted varied from 200 to 250 g, of either sex and randomized into two groups, 5 rats in each.METHODS: In the control, the rats were bred in the room environment for 7 days. In hyperoxic preconditioning (HP) group, the rats were placed in hyperbaric oxygen chamber and inhaled oxygen of 80％ to 85％ (101. 325 kPa) everyday for 6 hours, continuously for 7 days. At the end of experiment, the lung was collected to measure lung wet mass. 0.4 g pulmonary tissue was collected to prepare homogenate in freezing bath and its upper clear solution was collected to determine the activities of superoxide dismutase (SOD), glutathione peroxidase (GTTP) and catalase with chemiluminescence method successively. Pulmonary tissue was fixed with 30 g/L glutaradldehyde and prepared into sections to be observed the microstructure of lung under electron microscope.MAIN OUTCOME MEASURES: ① Comparison of lung wet mass and activities of SOD, glutathione peroxidase and catalase in pulmonary tissue in two groups. ② Alternation on microstructure of pulmonary tissue.RESULTS: Ten rats were all entered result analysis. ① Activities of SOD,GTTP and catalase in pulmonary tissue: Those in HP group were all higher remarkably than the control [(185.10±1.92) ％/g, (90.50±4.17) nkat/g,(4.38±0.18) k/g; (95.15±1.43) ％/g, (20.17±2.17) nkat/g, (1.10±0.19) K/g,t= -84.016, -33.488, -28.023, P ＜ 0.01]. ② Lung wet mass: That in HP group was near to the control [(1.32±0.07), (1.30±0.03) g, P ＞ 0.05], explaining that the fluid in the lung of HP group was not increased. ③ Microstructure of pulmonary tissue: In HP group, the basement membrane of pulmonary tissue was integrative and thickness was even, vascular endothelial cell was integrative and Ⅰ-type epithelial cell was not destroyed.CONCLUSION: Intermittent high concentration oxygen improves antioxidase activity in the lung of rat and does not injure the lung obviously.

Objective: To study the right hemisphere function state of NLD children. Methods: Adapting tachistoscopic vision and Benton Revised Visual Retention Test (VRT), three groups involving 20 children each were studied. Results: Under the tachistoscopic vision, NLD children achived poorly in recognition of nonverbal stimulus; and they do worse also in VRT test, with more errors of omission and distortion. Conclusion: Compared with normal children, the function of NLD children's right hemisphere is relatively weaker.

Objective To discuss the practicability of application of fault tree analysis in innovation to electrical system of SL-IE radiotherapy simulator.Methods The structure and principle of the electrical system were analyzed,the fault tree for the electrical system was built,and the qualitative analysis and quantitative analysis to the fault tree were made.The electrical system was innovated with effective redundant method,and the qualitative analysis and quantitative analysis were made after innovation.Theoretical analysis and the actual validation were made for the fault tree of electrical system after innovation.Finally,several notes were provided for the innovation to the similar medical equipments.Results The fault tree analysis method was applied to innovating the electrical system,and theoretical analysis indicated that the failure probability to total system reduced obviously.Actually,the actual effect after innovation also validated the correctness of the theoretical analysis.Conclusion Application of fault tree analysis in innovation to electrical system of SL-IE radiotherapy simulator is practical.But,the selection to the innovation event,the change to maintainence after innovation and the event to innovate carefully should be paid attention to.

In order to study the values of MR/CT to the diagnosis, differential diagnosis, treatment and prognosis of systemic lupus erythematosis with neuropsyehiatric disorders (SLE-NP), MR/CT image changes of 22 examinations of skull/spinal cord in 16 patients with SLE-NP were analyzed. The results showed that the images of 18 examinations were abnormal (81.8%), including ischaemia/infarct (9 times), haemorrhage (3 times), focal demyelination (3 times), and cerebral atropy (6 times). The areas involved including basal ganglia (10 times), anterior horn of lateral ventricle (5 times), pons (1 time), and spinal cord (2 times). MR/CT is sensitive for the examination of lesions of SLE-NP, and the total positive rate is as high as 81.8%. Its high resolution rate can display the nature and areas of the lesions, which may provide valuable reference for the early diagnosis, differential diagnosis, treatment, evaluation of therapeutic efficacy and prognosis of SLE-NP.

OBJECTIVETo compared surgical and nonsurgical treatment of clavicular midpiece fracture.

METHODSFrom September 2002 to September 2011, 254 adult cases of displaced midshaft clavicle fractures were retrospective analyzed, including 188 males and 66 females who ranged in age from 19 to 62 years (mean 39.3 years). There were 38 cases of transverse fracture, 71 cases of oblique fracture,and 145 cases of comminuted fracture. All cases were fresh single fractures. According to the treatment methods, these patients were divided into conservative group and surgical group. In conservative group, 127 patients (96 male and 31 female; mean age 40.1 +/- 6.02) were treated by manipulative reduction and fixation with "infinity" shaped bandage. In surgical group, .96 patients (92 male and 35 female; mean age 39.0 +/- 5.11) were treated by open reduction and internal fixation with reconstruction plates. Appearance satisfaction, the cost of treatment and the incidence of complications were compared between the two groups. Therapeutic efficacy was evaluated according to Han's standard.

RESULTSAll patients of both groups were followed up for 8-10 months (mean 9.8 months). In surgical group,the curative effect was excellent in 110 cases, good in 12 cases and fair in 3 cases; in conservative group, the curative effect was excellent in 62 cases,good in 28 cases and fair in 37 cases. The therapeutic efficacy in surgical group was better than that in conservative group. The appearance satisfaction of conservative group was higher than that of surgical group. The cost of treatment and complications were lower in conservative group than those in surgical group.

CONCLUSIONTreatment modalities for adult clavicle fractures should be selected on the basis of comprehensive analysis. Non-surgical treatment should be the treatment of choice, and indication for surgical treatment should be controlled strictly.

Adult ; Case-Control Studies ; Clavicle ; injuries ; surgery ; Female ; Fractures, Bone ; therapy ; Humans ; Male ; Middle Aged ; Retrospective Studies

OBJECTIVETo develop an RP-HPLC method for simultaneous determination of 5 constituents in Fructus Cnidii.

METHODAnalysis was performed on an Alltech C18 (4.6 mm x 250 mm, 5 microm) column. The mobile phases were acetonitrile water and acetic acid with gradient elution. The flow rate was 1 mL x min(-1). The monitoring wavelength was 325 nm and 245 nm. The column temperature was 40 degrees C.

RESULTThe linear response ranges were 1-20 microg x mL(-1) (r = 0.999 9) for xanthotoxin, 1-20 microg x mL(-1) (r = 0.999 9) for isopimpinellin, 11-20 microg x mL(-1) (r = 0.999 8) for bergapten, 100-1 200 microg x mL(-1) (r = 0.999 7) for imperatorin, 100-2 000 microg x mL(-1) (r = 0.999 9) for osthole. The average recoveries were all above 95%.

CONCLUSIONThe method is simple, sensitive and accurate with good reproducibility.

Chromatography, High Pressure Liquid ; methods ; Cnidium ; chemistry ; Coumarins ; analysis ; Fruit ; chemistry ; Furocoumarins ; analysis ; Methoxsalen ; analogs & derivatives ; analysis ; Plants, Medicinal ; chemistry ; Reproducibility of Results

Objective To study the Mediating effect of insecurity between childhood abuse and emotional distress of medical students,and to provide basis to intervene their mental disorder.Methods Through stratified sampling,262 medical students in Jiangsu were investigated by the Personal Report of Childhood Abuse( PRCA),Self-Rating Feeling of Insecurity Scale( SRFIS),Beck Depression Inventory( BDI ),Self-rating Anxiety Scale(SAS)and Suicide Ideation Scale(SIS).A path analysis was applied by Analysis of Moment Structures(AMOS) version 7.0.Results The path analysis showed that childhood abuse was directly related to insecurity of medical students ( β =0.538,P ＜0.01 ),and was directly related to emotional distress ( β =0.435,P ＜ 0.01 ).Insecurity were directly related to emotional distress of medical students ( β =0.342,P ＜ 0.05 ).Insecurity mediated partly the relationship between childhood abuse and emotional distress of medical students.The model fit indexes were X2/df =1.365 ＜ 3.000,P =O.082 ＞ 0.05,RMSEA =0.037 ＜ 0.050,GFI =0.971,AGFI =0.940,NFI =0.943,RFI =0.902,IFI =0.984,TLI =0.972,CFI =0.984.Conclusion Insecurity as mediated variable mediates the relationship between childhood abuse and emotional distress of medical students.

Objective To study the expression of transcription factor FOXO1 and FOXO3a on peripheral blood mononuclear cells (PBMC) in patients with active or inactive systemic lupus erythematosus (SLE) and investigate the effect of FOXO1 and FOXO3a on the clinical features of SLE. Methods Thirty SLE patients and 10 healthy controls were enrolled. PBMC were separated from the peripheral blood. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot were applied to analyze the expression of FOXOI and FOXO3a. Results The level of FOXO1 expression was significantly decreased in active SLE patients compared with controls and patients with inactive SLE (P<0.05). The level of FOXO1 expression in inactive SLE patients was lower than that of the controls (P<0.05). The expression of FOXO1 mRNA was negatively correlated to SLEDAI. However, the level of FOXO3a was similar among the three groups. Conclusion The result suggests that FOXO1 may be involved in the pathogenesis of SLE and the expression level of FOXOI may be a good indicator for the disease activity of SLE.

Objective Serological characteristic analysis for patients with autoimmune hemolytic anemia(AIHA)who has cold and warm auto‐antibody when undergoing blood type and cross‐matching .Methods Choosed 3 donors of red blood cells of group O to absorb autoantibodies of the patient′s serum(by using the methods of cold absorption) and used 2‐mercaptoethanol(2‐Me) to de‐stroyed the IgM antibody .Results The patient of AIHA blood type was B+ .There was cold and warm auto‐antibody in the pa‐tient′s serum ,and the result of cross‐matching is incompatible .Conclusion The patient′s serum contains cold and warm auto‐anti‐body which interferes blood type and cross‐matching .In emergency ,considering the patients condition ,blood transfusion combined with the drug treatment could achieve good effects .

Objective To contrastively analyze the performance of two kinds of blood cell separators for apheresis platelets . Methods 80 blood donors were selected from 4 234 healthy apheresis platelet donors and their data before and after apheresis platelets were respectively collected by using two kinds of different blood cell separators Trima and Amicus .The product quality during the collection process ,residual WBC and RBC count ,acquisition time ,collection efficiency ,scrapping situation and safety were performed the comparative analysis between the two kinds of blood cell separator .Results In the case of the difference of the basic parameters before apheresis platelet having no statistical significance ,the two kinds of blood cell separator had no differences in the aspects of product quality ,processing blood amount and the use amount of anticoagulants(P>0 .05);but there were statisti‐cal differences in the aspects of acquisition time ,collection efficiency and pipeline residual blood amount (P<0 .05) .Conclusion Two kinds of blood cell separators have their own advantages and disadvantages during the use process ,so the blood cell separator should be selected according to the physical quality and their own characteristics of blood donors .