Objective To investigate the clinical safety and effect of transurethral plasmakinetic enucleation and resection of prostate combined with mini-incision in the treatment of senior and high-risk benign prostatic hyperplasia (BPH) with bladder stones. Methods Clinical data of 42 patients of senior and high-risk benign prostatic hyperplasia (BPH) with bladder stones was analyzed. All patients were treated with transurethral plasmakinetic enucleation and resection of prostate combined with mini-incision cystofithotomy. Results All operations were successfully performed without deaths. The operative time was 52~86 min, mean (63.90 ± 16.60) min. The blood loss was 45.0~136.0 ml, mean (73.90 ± 21.10) ml. There were no significant differences in the immediate life characteristics, serum K+, serum brain natriuretic peptide and other internal environmental indexes before and after operation. There were no serious complications in perioperation. 3 months after operation, international prostate symptomscore (IPSS), quality of life score (QOL), the maximum flow rate (Qmax) improved significantly compared with preoperation, significantly reduced the post void residual volume (PVR). Conclusion Transurethral plasmakinetic enucleation and resection of prostate combined with mini-incision can be particularly applied to elderly patients with high risk BPH complicated with bladder stones with efficaciously and more safety.

Objective To improve the diagnosis and treatment of retroperitoneal liposarcoma .Methods We reported one case of huge retroperitoneal liposarcoma in our hospital .The clinical characteristics ,diagnosis and treatment were discussed ,and the literatures were reviewed .Results The patient received the left retroper-itoneal tumor resection and partial resection of the left kidney after the imaging diagnosis .Postoperative pathologi-cal results indicated retroperitoneal liposarcoma .No tumor recurrence and metastasis ocurred in the follow -up of 20 months.Conclusion Retroperitoneal liposarcoma is rare ,with no specific clinical manifestations .The applica-tion of CT is helpful to the diagnosis of this rare disease .Surgery is the preferred method for the treatment .

AIM:To explore the effects of PPARγon the elevated level of reactive oxygen species ( ROS) in-duced by high glucose and its mechanism .METHODS:Human umbilical vein endothelial cells ( HUVECs) were cultured with DMEM containing high glucose (33 mmol/L D-glucose), and DMEM containing lower glucose (5.5 mmol/L D-glu-cose) was used as control .Superoxide anion and nitric oxide fluorescence probes were used to observe the effects of PPAR γagonist on ROS and NO productions in the HUVECs .The uncoupling protein 2 (UCP2) protein level in the HUVECs was detected by Western blotting .RESULTS:PPARγagonist pioglitazone inhibited the ROS generation and prevented the de-crease in NO level under high glucose condition , and these effects were reversed by pretreatment with PPARγantagonist GW9662.The results of Western blotting indicated that PPARγagonist pioglitazone up-regulated the UCP2 expression un-der high glucose condition , and this effect was also blocked by GW 9662.Inhibition of UCP2 by genipin attenuated the effect of pioglotazone on the ROS production .CONCLUSION: Activation of PPARγinhibits ROS generation under high glucose condition , and this effect may mediate by up-regulation of UCP2.