Objective:To study the effects of ?-GABA on the release of the soluble amyloid precursor protein(sAPP).Methods:Male Sprague-Dawley rats were decapitated and hippocampus was isolated at4℃,then hippocampal slices were quickly prepared in the medium of Krebs-Ringer buffer at0-4℃by a vibration chopper.After the slices were preincubated in Krebs-Ringer buffer at37℃,they were stimulated with various concentrations of ?-GABA(1.0?10 -9 -1.0?10 3 ?mol/L)in Krebs-Ringer buffer.The supernatant was removed from the solution after centrifugated at15000?g.Quantities of total pro-tein were measured by bicinchoninic acid(BCA)assay.The sAPP released into the incubation medium were assayed by West-ern blot.Results:?-GABA increased sAPP release in the incubated hippocampal brain slices.However,it failed to produce special protein.Conclusion:?-GABA within certain concentration range has inhibitory effects on the sAPP release.[