Objective:To establish the method for the determination of five anthraquinones constituents including aloe-emodin , rhein, emodin , chrysophanol and physcion in Yanxiaodinaer syrups. Methods: An HPLC method was used with a DIONEX Acclaim 120A C18 (250 mm × 4. 6 mm,5 μm) column. The mobile phase was acetonitrile-0. 1% phosphoric acid with gradient elution at flow rate of 1. 0 ml·min-1 . The detection wavelength was 254nm,the column temperature was 30℃and the injection volume was 10 μl. Results:The linear ranges of the five anthraquinones were 0. 018 7-0. 467 0 μg(r=0. 999 8),0. 020 2-0. 504 0 μg(r=0. 999 9), 0.013 0-0.390 6 μg(r=0.999 9),0.013 3-0.399 9 μg(r=0.999 9)and 0.009 3-0.277 8 μg(r=0.999 9), and the recoveries were 94. 30%(RSD=2. 68%,n=6),95. 49%(RSD=2. 50%,n=6),93. 70%(RSD=2. 42%,n=6),92. 52%(RSD=2. 40%,n=6) and 93. 72%(RSD=2. 54%,n=6), respectively. Conclusion:The method is accurate , sensitive and reproducible, which can be used as the basis for the quality control of Yanxiaodinaer syrups.

Objective: To explore self-other agreement on personality and moderation effects of relationship quality.Methods: 114 pairs of previously unacquainted freshmen roommates accomplished the Chinese Personality Scale(QZPS) at the second week and the 15th week subsequent to the initial introduction.Results: Although all self-other agreement coefficients were different for all personality trait scores in QZPS between the 2nd week and the 15th week,some coefficients increased from the 2nd week to the 15th week,others didn’t increased but decreased from the 2nd week to the 15th week,and all the increase was not statistically significant.At the second week,main effects and moderator effects of relationship quality was not significant for all personality traits.At the fifteenth week,main effects of relationship quality were significant for kindness and human relations,and moderator effects of relationship quality were significant for extraversion and ways of life.Conclusion: There is no acquaintanceship effect demonstrated by many western literatures in Chinese culture,but moderator effects of relationship quality are significant in some traits.

Objective To explore the clinical efficacy and safety of low dose fentanyl combined to ropivacaine in subarachnoid block for cesarean section.Methods Eighty patients with ASA physical status Ⅰ and Ⅱscheduled to undergo cesarean section for delivery were randomly allocated to receiving spinally mixture none fentanyl ( group R),5 μg fentanyl ( group F5 ), 10 μg fentanyl ( group F10 ), and 15μg fentanyl ( group F15 ), combined with 1%ropivacaine 1 ml and 10% glucose 1 ml respectively,after combined spinal-epidural anesthesia puncture was applied at the L2～3 interspace.Heart rate, blood pressure, the onset and recovery time of the sensory and motor block,time to the highest level and the highest level of sensory block, time to the maximal extent and the maximal extent of the motor block were recorded at regular intervals.The adverse effects were sought during and after surgery.Neonatal outcome was assessed using Apgar score.Results The recovery of sensory block was prolonged significantly in group F10 and group F15 than in group R and group F5.Conclusion Ropivacaine with 10μg fentanyl is safe and effective in subarachnoid block for cesarean section.

Objective To predict the occurrence of hypoparathyroidism following total thyroidectomy. Methods In this study, 124 patients underwent total thyroidectomy, 46 for thyroid cancer and 78 for multinodular goiter, additional neck dissection was performed on cancer patients. Serum calcium and parathyroid hormone (PTH) levels were examined preoperatively and at 1 h, 1 d and 2 d postoperatively. The occurrence of postoperative hypoparathyroidism was observed. Receiver operating characteristic curve analysis was employed to identify the best indicator to early predict the occurrence of clinical hypocalcemic symptoms. Results Fifty-eight (46.8%) patients suffered from postoperative transient hypoparathyroidism, with 22 ( 47. 8% ) cases in thyroid cancer group and 36 ( 46. 2% ) in multinodular goiter group ( λ2 = 0. 033, P = 0. 857). One (0.8%) patient in cancer group had permanent hypoparathyroidism. 90 patients (72.6%) had postoperative hypocalcaemia, 58 (46. 8% ) had subnormal serum PTH levels, 40 (32. 3% ) had hypocalcaemia symptoms. Postoperative serum calcium (F=21. 358,P =0. 000) and PTH ( F = 18.253, P =0.000) levels decreased more in cancer group than in goiter group.Receiver operating characteristic curve analysis demonstrated that the percentage of serum PTH level decline at 1 h postoperatively was most predictive and 76. 6% decline was the best cut-off value for the occurrence of clinical hypocalcaemia symptoms ( area under the curve being 0.933 ) with a sensitivity of 89. 7% and a specificity of 87.9%. Conclusions Neck dissection added to total thyroidectomy can decrease the postoperative serum calcium and PTH levels more seriously, but may not increase the incidence of postoperative transient hyperparathyroidism. The percentage of serum PTH level decline at 1 h postoperatively predicts the occurrence of clinical hypocalcaemia symptoms.

Objective To evaluate the effect of red light irradiation on serum phosphorus reduction in hemodialysis.Methods Sixty maintenance hemodialysis patients were divided into treatment group and control group.During the hemodialysis,the blood in the extracorporeal circulation tube of the patents in the treatment group was irradiated with red light by a MRX-1 red light therapy system.The irradiation was continued for 60 minutes each time,and one course of the treatment contained 10 times of irradiations.Patients in the control group were subjected to hemodialysis by conventional methods.The serum phosphate levels of all patients were measured before and after the treatment.Results The symptoms of dialysis disequilibrium of the patients in the treatment group were alleviated.There was no significant difference in serum phosphate levels between the treatment group and the control group before hemodialysis,while a statistically significant difference was found after the treatment (P＜0.05).Conclusions Hemodialysis combined with red light irradiation on external blood trails can contribute to the decrease of serum phosphate levels in maintenance hemodialysis patients.

OBJECTIVE:To analyze the characteristics and rules of new and serious ADR induced by Chinese patent medicine in Wuhan during 2012-2013,and to improve the monitoring levels of Chinese patent medicine-induced ADR and clinical rational drug use. METHODS:New and serious Chinese patent medicine-induced ADR cases reported by 16 districts of Wuhan during 2012-2013 were classified and analyzed statistically. RESULTS:A total of 245 cases of new and serious TCM ADR were reported in 2012-2013,accounting for 13.61% of all reports;the incidence of ADR in patients above 51 years old was the highest,account-ing for 55.51%;42.04%of new and serious ADR induced by Chinese patent medicine occurred within 30 minutes after using medi-cine;among suspected drugs,intravenous dripping was the main way to cause new and serious ADR induced by Chinese patent medicine (50.39%);blood-regulating formula was the main cause of new and serious ADR induced by Chinese patent medicine (40.80%);new and serious ADR induced by Chinese patent medicine mainly manifested as systemic damage(25.97%),followed by lesion of skin and its appendants(18.81%). CONCLUSIONS:It is needed to strengthen the rational use of Chinese patent medi-cine and the supervision of TCM injection for activating blood circulation to dissipate blood stasis,strengthen the supervision and improvement of instruction content of Chinese patent medicine. TCM Pharmacists should carry out the clinical pharmaceutical care.

Objective:To study and design measurement scale which can judge and survey sub-health state and itscharacteristic of TCM syndrome.Methods:Making use of methods of scale technique,literature research,expertise consultation et al,based on TCM theory,via conceptualization and operation of sub-health and TCM syndrome,forming structure and type of scale,establishing item pool and results,and then through item selection,assessment of validity and reliability of pre-investigation,making the measurement scale.Results:The scale is made up of 4 parts:body function state,psychological function state,social function state and particular state of TCM.The 4 parts are divided into 15 sorts,including 120 items.Conclusion:The scale contributes much to the diagnosis of sub-health state and the clari cation of clinical manifestations,features,distributions of TCM syndrome of public of sub-health,which is one of the most important methods for investigating TCM syndrome of sub-health state.

BACKGROUND:Posterior maleolar fracture is an important factor affecting the prognosis of ankle fractures. Posterior maleolar fracture often caused by high energy trauma. Using what kind of fixation and fixation materials biomechanics, and how to embed has become a current research hotspot. OBJECTIVE:To compare the clinical outcomes of different fixation materials on posterior maleolar fracture, and analyze the effect of different fixation methods on biomechanical outcome of posterior maleolar fracture fixation, so as to provide a basis and reference of selecting the best fixation for the clinical treatment of ankle fracture. METHODS: The relevant literature included by PubMed database and the China National Knowledge database from the year of 1976 to 2015 were retrieved by the first author through computer. English key words are “Ankle fracture; internal fixation; biomechanics; biocompatibility”, Chinese language search terms are “posterior maleolar fracture; internal fixation; biomechanics; biocompatibility”. Summarize the most commonly used metal fixation materials and absorbable content materials. The clinical commonly used metal fixation materials including bone plate and screws, absorbable fixation materials such as biodegradable material polylactic acid, polyethylene plastic ester, polylactide gum ester, etc. The biomechanical properties were analyzed. RESULTS AND CONCLUSION: The new locking screw can withstand more buckling and shear force. Lag screw has a tapping action, and the puling was stronger after tapping, but for patients with osteoporosis, the pressure effect of screw on fracture fragments was limited, the strength was not enough, at this time, locking plate should be used. Locking plate has a greater stability and higher confrontational feature to bending stress, less likely to pul out. Steel coupling screw provides better stability. To avoid secondary removing of the fixation, stress protection after fixation and other shortcomings after the metal material fixation fracture healing, the strength of the absorbable fixation material made by biodegradable material polylactic acid, polyvinyl acetate glue and polypropylene plastic ester after high temperature and pressure processing was increased. Absorbable fixation material may overcome the adverse effects associated with metal fixation, such as imageological examination, secondary implant removal. These results show that the mechanical properties of the absorbable screws are more closer to human bone, no surrounding bone vulnerability due to stress shields, no osteoporosis occurs, and can degrade in the body, but its fixation strength is stil less than the traditional fixed screw and bone plate. We should choose a suitable fixation material according to the forces of fracture site and the size of the fracture fragments.

Objective To detect the expression of miR-26a/b in the aorta and serum of mice so as to explore the role of miR-26a/b in vascular remodeling of hypertension.Methods C576L/BJ male mice were randomly divided into AngⅡ group and control group.Mini-osmotic pump was implanted subcutaneously into the back of mice, and the model of blood vessel remodeling in mice was established by continuous infusion of AngⅡ(2.0mg/kg·d).The mice in control group were injected with saline.Blood pressure was taken before the intervention and at 3, 5, 7, 10 and 14 days after the intervention.After 2 weeks, the mice were killed, the serum and aorta tissues were collected, and the expression of miR-26a/b was determined by RT-PCR.HE staining, Masson staining and immunohistochemistry were performed to observe changes in vascular morphology, fibrosis and protein expression.Results After the intervention, systolic blood pressure and diastolic blood pressure were significantly higher in AngⅡ group than in control group (P<0.05).HE staining showed that the vessel wall of AngⅡ group was thicker than that of control group.Masson staining showed more blue collagen deposition in the middle of aorta in AngⅡ group but no obvious collagen deposition in control group.RT-PCR showed that the expression of miRNA-26a/b in the serum and aorta of AngⅡ group was significantly lower than in control group (P<0.05).Immunohistochemistry indicated that the expressions of CTGF, collagen Ⅰ and collagen Ⅲ all increased after AngⅡ infusion (P<0.05).Conclusion MiR-26a/b, CTGF, collagen Ⅰ and collagen Ⅲ may be involved in AngⅡ-induced vascular remodeling in hypertension.MiR-26a/b may be a new therapeutic target of vascular remodeling in hypertension.