A simple, rapid and accurate spectrofluorometric method has been developed for determination of vitamin E in crop seeds. Several experiment conditions were discussed in detail. Linear analytical curve was obtained in concentration of 0-55ug of vitamin E in 10 ml solution. The precisions were 0.5-2.9% relative SD. The recoveries were 86-104%.

AIM To observe the effect of polysaccharide from spirulina platensis(PSP) on lung injured by exposing ozone. METHODS The injury model of lung were induced by exposing ozone. The xanthine oxidase method and TBA were used to determine SOD and MDA in serum and lung respectively. The transmission electron microscope was used to observe the structural change of lung cell. RESULTS Compared with model mice, SOD in the groups of PSP administrated mice were increased and MDA were decreased significantly(P

Objective To investigate the significance of the detection of cytomegalovirus (CMV) infection after kidney transplantation. Methods The serum CMV antibody(anti CMV) and CMV DAN of 72 recipients before and after transplantation were detected respectively by enzyme linked immunosorbent assay(ELISA) and polymerase chain reaction(PCR). Results The levels of serum CMV antibody and CMV DNA of 72 recipients after transplantation were significantly higher than those of pre operation( P

Objective To establish a method for the quantitative determination of sildenafil in aphrodisiacs by liquid chromatography-mass spectrometry (LC-MS). Methods Chromatography was carried on a Waters BEH C18 column (50 mmí2. 1 mm,1. 7 μm) with the column temperature being 35 ℃ and 0. 02 mol·L-1 ammonium acetate water solution (containing 0. 1% acetic acid)-acetonitril (7030) as the mobile phase at a flow rate of 0. 2 mL·min-1 . Determination was performed by multiple reaction monitoring mode in two channels,475→100,475→283,using electrospray ionization in positive ion mode. Results Sildenafil had a good linear relationship in the range of 0. 1986-1. 986 ng with the linear regression equation being Y=27 750X+15 232 (r=0. 995 2). The average recovery was 96. 6% with RSD being 1. 8% (n=9). The limits of quantitation and detection were 0. 04 ng and 0. 01 ng,respectively. Conclusion The determination results of 10 batches of samples show that the method is sensitive and accurate and can be used as an appropriate technique to detect sildenafil in aphrodisiacs.

Bruton tyrosine kinase (Btk) is a member of the non-receptor protein tyrosine kinases of Tec family.It plays an important role in growth,proliferation,differentiation and signal transduction of B lymphocyte.After activation of B-cell receptor (BCR) by diverse stimuli,Btk is activated through tyrosine phosphorylation.Activated Btk can stimulate several downstream signaling proteins.Btk and BCR signaling pathways play important roles in initiation and maintenance of B cell malignancies.This review focuses on the recent findings in association of Btk and B cell malignancies.

Objective To investigate the illness uncertainty state of the family members of elder patients with colostomy,explore their correlation with social support.Methods A total of 138 family members of elder patients with colostomy in two hospitals from September 2013 to November 2014 were recruited in this study.The General Information Questionnaire,Mishel Uncertainty in Illness Scale-Family Member Form (MUIS-FM) and Social Support Rating Scale (SSRS) were used to investigate the family members of elder patients with colostomy and analyse the correlation between illness uncertainty of family members and its influencing factors and social support.Results The average total score of illness uncertainty was (72.17±9.44) scores.There was significantly different of the family members of elder patients with colostomy in total uncertainty on degree of education,the relationship with patient,stoma nature and economic conditions (P＜0.05).The average total score of social support was (37.14±5.25) scores.The level of illness uncertainty was negatively associated with social support (r=-0.526,P＜0.01).Conclusions The illness uncertainty was mostly in higher levels in the family members of elder patients with colostomy and was negatively correlated with social support.Clinical practice should improve social support of family members to relieve illness uncertainty,so as to provide better services of family and promote physical and psychological rehabilitation of patients.

Objective: To observe the clinical effect of acupuncture and hydro-acupuncture in treating anxiety. Methods: Compare between the therapeutic effect of 186 cases of Three Huang Points of DONG's extra points in treating anxiety and that of 139 cases by purely Western medicine buspirone. Results: The total effective rate of the treatment group was 91.4%, and the rate of the control group was 93.5%. There was statistical difference of Hamilton Anxiety Scale (HAMA) and Self Rating Anxiety Scale (SAS) scores before and after treatment in both groups (P＜0.001), while there was no statistical difference between the two groups (P>0.05). Conclusion: Acupuncture treatment compared favorably to anti-anxiety drug buspirone. Oral administration of buspirone had different degrees of side effects, such as dizziness, dry mouth, constipation, difficulty urinating and acidophilus, while there was basically no side effects of acupuncture and the effect of acupuncture was better than buspirone. This was especially suitable for patients who could not take medications for various reasons.

Objective To investigate the relationship of pulmonary function and metabolic indexes in overweight as well as obesity people.Methods Three hundred and five health examination adults were selected as our subjects.The basic parameters,metabolic indexes and pulmonary function were measured.Of which,pulmonary function indexes include forced vital capacity (FVC),forced expiratory volume in one(FEV1),peak expiratory flow(PEF),the ratio of the forced expiratory volume in the first one second to the forced vital capacity (FEV1/FVC),the ratio of the forced expiratory volume in the first one second to the vital capacity(FEV1/VC),maximal expiratory flow after 50％ of the FVC (MEF50),maximal expiratory flow after 25％ of the FVC (MEF25),and each index value of lung function was expressed the ratio of the measured value/the predictive value.Metabolic indexes include triglycerides (TG),total cholesterol (TC),low-density lipoprotein cholesterol (LDL-C),fasting plasma glucose (FPG)),C-reactive protein (CRP),high-sensitivity C-reactive (hs-CRP),superoxide dismutase(SOD),systoloc blood pressure (SBP) and diastolic blood pressure (DBP).Statistical analysis methods include one-way analysis of variance and Spearman correlation analysis.Results The levels of FVC,FEV1,FEV1/FVC in overweight and the obesity group were (85.74 ± 13.94)％ and (82.85±13.34)％,(84.52 ± 14.62)％ and (82.74 ± 14.18)％,(103.40 ± 13.05)％ and (103.17 ±8.99)％respectively,lower than that of normal weight group [(95.79 ± 26.83) ％,(92.65 ± 26.93) ％,(99.98± 11.88) ％,all P values less than 0.05)].Compared with the normal weight group,the levels of TG,SBP,FPG in overweight group and the obesity group were significantly increased.The levels of LDL-C,DBP,hs-CRP in obesity were (5.05 ± 0.83) rmtmol/L,(86.64 ± 10.49) mmHg,(3.74 ± 5.51) mg/L respectively,higher than that of normal group [(3.08 ±0.96) mmol/L,(77.69 ± 13.20) mmHg,(2.33 ±4.67) mg/L,P ＜0.05)].SOD activities in overweight and obesity group were (140.82 ± 13.16),(144.89 ± 13.82) U/L respectively,significantly lower than that of normal weight group[(148.64 ± 14.94) U/L,P ＜0.05)).The levels of SBP,DBP,hs-CRP in the over weight group were (127.77 ± 19.07) mmHg,(80.87 ± 12.21) mmHg,(2.31± 3.73) mg/L),higher than that of obesity group.Among metabolic indices,TG,SBP,DBP,FBG,CRP,hs-CRP and SOD were related with FVC (r =-0.129,-0.129,-0.136,-0.180,-0.220,-0.217 respectively,P ＜ 0.05 or P ＜ 0.01).There was negatively correlated relationship between SBP,FBG,CRP,hs-CRP and FEV1 (r =-0.128,-0.127,-0.148,-0.198 respectively,P ＜0.05 or P ＜0.01),So were SBP,CRP,hs-CRP and PEF (r =-0.137,-0.117,-0.133 respectively,P ＜ 0.05).Negatively correlated relationship between hsCRP,SBP and MEF50 were seen (r =-0.126,-0.124,P ＜ 0.05).Meanwhile there was negatively correlated relationship between SOD and FVC,FEV1/FEV,PEF,MEF50 (r =0.149,0.094,0.119,0.141,0.129respectively,P ＜ 0.05 or P ＜ 0.01).Conclusion Impaired pulmonary function and metabolic disorders were showed in the overweight and obesity people.Metabolic indexes were related with pulmonary function.

Objective To understand the paroxysm spreads characteristic, among Shenzhen community, and to offer some reference about preventing STD. Methods analyze STD in Pingshan community of Shenzhen according to the data in the disease reports from 2005 to 2007 by descriptive Epidemiology method. Results In the disease re-port of Pingshan community in Shenzhen City,there are 160 cases of t6he STD in 2005. 182 cases in 2006. 145 cases in 2007. Incidence rate is 1.683‰, 1.225‰, 1.710‰. Total incidence rate is 1.522%‰, male incidence rate is 1.082‰,female incidence rote is 0.441‰; Among 6 kinds STD, the ratio of gonorrhea is highest, occupying 28.13%. Different sex has different ratio. The male gonorrhea ratio is highest,occupying 34.97%, but the female syphilis is highest,occupying 48.94% ;6 kinds of sexual infectant diseases root in having sex without marrying and it accupied. 69.83%. The origin of disease has difference in sex. Conclusions The incidence of a disease of sexual infectant is a bit high. The main way of transmit is sex osculation without marrying and the inborn syphilis The HIV of pregant woman is not very well,promoting to increase the drumbeating work of sex healthy education,giving publicity to keep your self-esteem gereatly Lets use as many condoms as possible in that sination. The Physical examination of sexual infectant diseases for the pregant women should be strengthended.

Congenital cardiovascular anomalies are present in approximately 80％ of children with 22q11.2 deletion syndrome.Three genes in chromosome 22q11.2 ( TBX1,CRKL,and ERK2 ) have been identified whose haploinsufficiency causes anomalies of 22q11.2 deletion.The most common diseases are conotruncal anomalies,which include tetralogy of Fallot ( TOF),pulmonary atresia with ventricular septum defect (PA-VSD),truncus arteriosus,and interrupted aortic arch.In major phenotypes,a high prevalence of the deletion is noted in patients with TOF with pulmonary atresia,TOF associated with pulmonary atresia and major aortopumonary collateral arteries,persistent truncus arteriosus,and type B interruption of aortic arch.In minor phenotypes,right aortic arch,aberrant subclavian artery,and major aortopulmonary collateral arteries are frequently associated with cardiovascular anomalies associated with 22q11.2 deletion.In conclusion,conotruncal anomaly associated with aortic arch and branch anomalies should increase the suspicion of 22q11.2 deletion.