Objective To investigate the illness uncertainty state of the family members of elder patients with colostomy,explore their correlation with social support.Methods A total of 138 family members of elder patients with colostomy in two hospitals from September 2013 to November 2014 were recruited in this study.The General Information Questionnaire,Mishel Uncertainty in Illness Scale-Family Member Form (MUIS-FM) and Social Support Rating Scale (SSRS) were used to investigate the family members of elder patients with colostomy and analyse the correlation between illness uncertainty of family members and its influencing factors and social support.Results The average total score of illness uncertainty was (72.17±9.44) scores.There was significantly different of the family members of elder patients with colostomy in total uncertainty on degree of education,the relationship with patient,stoma nature and economic conditions (P＜0.05).The average total score of social support was (37.14±5.25) scores.The level of illness uncertainty was negatively associated with social support (r=-0.526,P＜0.01).Conclusions The illness uncertainty was mostly in higher levels in the family members of elder patients with colostomy and was negatively correlated with social support.Clinical practice should improve social support of family members to relieve illness uncertainty,so as to provide better services of family and promote physical and psychological rehabilitation of patients.

Objective: The Behavior Preference for Network Game Questionnaire (BPNGQ) was developed. The aim of this study was to provide a measurement for cyberpsychology. Methods: Through interview to players, we formed the pretest of BPNGQ. Then 422 players did the pretest, and 324 players did Internet Addiction Inventory (IAI) at the same time. Results: The item difficulties of all items ranged from 0.2 to 0.7. The discriminations of 70.18% items were good. Factor analyses divided scales into five factors, which were energy of devotion, emotion of devotion, behavior of aggression, emotion of aggression and attitude of aggression. The reliabilities of the subscales and full scale ranged from 0.70 to 0.92. The scores of subscales were significantly positively related with Internet addiction. Conclusion: BPNGQ’s difficulty is appropriate, and it has good discrimination, reliability and validity.

Objective:This research mainly concerns the relationship between the behavior preference in network game and the trait of personality of players.Methods:This study surveyed 294 players by Behavior Preference in Network Game Questionnaire(BPNGQ) and Eysenck Personality Questionnaire(EPQ).Results:All scores in factors of BPNGQ ex-cluding emotion of agression had significant correlations with all dimensions of EPQ.In all traits of personality,there was significant differences between types of killer and relaxing,and no significant difference between catharsis and relaxing.Sanguineous players had lower scores in putting in energy and emotion of devotion.Bilious players had higher scores in behavior of aggression,and players of viscous had higher scores in attitude of aggression.Conclusion:Theory of behavior preference in network game has evidence of personality.Players of different personality traits display various preference in network game.

Objective To identify and clone rat aging-related genes to provide clues for human aging mechanism. Methods Improved different display reverse transcript-PCR method was applied to identify differentially expressed genes in cortex tissues of 4-month and 24-month old BALB/c mouse. Results Forty-two cDNA fragments with differential expression were identified, and 21 with increase and 21 with decrease of expression in old mice. Among them, 17 represented genes with known protein function, 12 represented known gene sequences but their protein function was unknown, and the other 13 probably belonged to new cDNAs. Among the genes with known protein function, 2 genes were associated with oxidative stress, 3 with energy production, and 4 with protein metabolism, respectively. Additionally, gene expression alterations were also found in those related to cell apoptosis, neurodegenerative disorder, and growth and development regulation. Conclusions Rat aging might be related with the alteration of oxidative stress status, energy production and protein metabolism.

Objective: To investigate the possible association between the allelic variation of LAPTM4B and the genetic susceptibility of lung cancer. Methods: The genotype of LAPTM4B was analyzed in 134 unrelated healthy adult individuals and 166 patients with lung cancer by utilizing polymerase chain reaction based on special primers. The genotypical distribution of LAPTM4B was analyzed by ? 2 test. Results: The allelic frequencies of the *2 were 40.1% and 28.0% in the lung cancer group and the healthy control group respectively, which was significantly different between the two groups (P 0.002). There was a significant difference in the overall genotypical distribution between the patients and the controls (P0.005). The risk of suffering from lung cancer was increased 1.91 times in the individuals of the *1/2 genotype (95%CI: 1.178-3.110) and 3.26 times in the individuals of the *2/2 genotype of LAPTM4B (95%CI:1.338-7.929) compared with the *1/2 genotype. No association was observed between the genotypical distribution of LAPTM4B and the clinical information on patients of lung cancer such as gender, age, pathological type, differentiation classification of TNM and infection of HBV. Conclusion: This study suggests that the allele *2 of LAPTM4B might be the risk factor of lung cancer, which could be associated with genetic susceptibility of lung cancer.

Objective To establish a method for the quantitative determination of sildenafil in aphrodisiacs by liquid chromatography-mass spectrometry (LC-MS). Methods Chromatography was carried on a Waters BEH C18 column (50 mmí2. 1 mm,1. 7 μm) with the column temperature being 35 ℃ and 0. 02 mol·L-1 ammonium acetate water solution (containing 0. 1% acetic acid)-acetonitril (7030) as the mobile phase at a flow rate of 0. 2 mL·min-1 . Determination was performed by multiple reaction monitoring mode in two channels,475→100,475→283,using electrospray ionization in positive ion mode. Results Sildenafil had a good linear relationship in the range of 0. 1986-1. 986 ng with the linear regression equation being Y=27 750X+15 232 (r=0. 995 2). The average recovery was 96. 6% with RSD being 1. 8% (n=9). The limits of quantitation and detection were 0. 04 ng and 0. 01 ng,respectively. Conclusion The determination results of 10 batches of samples show that the method is sensitive and accurate and can be used as an appropriate technique to detect sildenafil in aphrodisiacs.

Objective: To observe the clinical effect of acupuncture and hydro-acupuncture in treating anxiety. Methods: Compare between the therapeutic effect of 186 cases of Three Huang Points of DONG's extra points in treating anxiety and that of 139 cases by purely Western medicine buspirone. Results: The total effective rate of the treatment group was 91.4%, and the rate of the control group was 93.5%. There was statistical difference of Hamilton Anxiety Scale (HAMA) and Self Rating Anxiety Scale (SAS) scores before and after treatment in both groups (P＜0.001), while there was no statistical difference between the two groups (P>0.05). Conclusion: Acupuncture treatment compared favorably to anti-anxiety drug buspirone. Oral administration of buspirone had different degrees of side effects, such as dizziness, dry mouth, constipation, difficulty urinating and acidophilus, while there was basically no side effects of acupuncture and the effect of acupuncture was better than buspirone. This was especially suitable for patients who could not take medications for various reasons.

Bruton tyrosine kinase (Btk) is a member of the non-receptor protein tyrosine kinases of Tec family.It plays an important role in growth,proliferation,differentiation and signal transduction of B lymphocyte.After activation of B-cell receptor (BCR) by diverse stimuli,Btk is activated through tyrosine phosphorylation.Activated Btk can stimulate several downstream signaling proteins.Btk and BCR signaling pathways play important roles in initiation and maintenance of B cell malignancies.This review focuses on the recent findings in association of Btk and B cell malignancies.

Objective: To evaluate the prognostic value of serum troponin Ⅰ (cTnI) in unstable angina pectoris (UA). Methods: Serum cTnI, creatine kinase isoenzyme MB (CK－MB) were simultaneously measured in 25 healthy subjects, 96 UA patients, routine treatment was administered and cardiac events were observed closely within two weeks. Results: Thirteen of 28 UA patients whose cTnI was≥0.3μg/L developed cardiac events, only 3 of 68 UA patients whose cTnI was ＜0. 3 μg/L developed cardiac events within two weeks, there was a significant difference (P＜0. 05). Conclusion: There is a clinical value of serum cTnI measurement in identifying the high risk patients of UA. The patients whose cTnI was≥0. 3μg/L indicate a poor prognosis.