Objective To observe the velocity of left ventricular wall motion features of normal subjects and patients with abnormal regional wall movement of left ventricle with the quantitative tissue velocity imaging(QTVI),and to know whether variances exit between the velocity of left ventricular myocardial motion of the normal subjects and the abnormal subjects with coronary artery disease(CAD) and myocardial ischemia. The practical value of QTVI on myocardium movement of left ventricle was evaluated. Methods The segmental left ventricular wall motion velocity was measured on 27 normal subjects and 21 patients with CAD and myocardial ischemia,including three waves,peak velocity of systolic S wave,diastolic E wave,A wave, and time velocity integrate of S wave(S TVI) was used as measurement parameters.The parameters were analysed between the two groups. Results The velocity and S TVI were gradually decreased from base to apex in the same wall in the control group.There were some abnormal segmental movements in the abnormal group. S wave ,E wave and S TVI of diseased segments in the abnormal group were much lower than those of control group ( P  0.05 ). Conclusions QTVI can quantitatively measure the velocity in abnormal movement segment of ventrical wall of patients with CAD and myocardial ischemia.S wave,E wave and S TVI are better parameters of assessing systolic function of left ventricle.QTVI has clinical application in regional myocardial motion function evaluation.

Background:Ulcerative colitis-associated colorectal cancer(UcCRC)is the most serious complication of ulcerative colitis(UC). 5-Aminosalicylic acid(5-ASA)could reduce the risk of UC progressing to UcCRC. Aims:To investigate the effect of 5-ASA on cell proliferation,apoptosis and cell cycle in human colon cancer cell line HT-29 for verifying the inhibitory effect of 5-ASA on UcCRC. Methods:HT-29 cells were treated with different concentrations(0,10,20,30, 40 mmol/ L)of 5-ASA. Cell proliferation was measured by CCK-8 assay. Cell apoptosis was determined by TUNEL method. Cell cycle was analyzed by flow cytometry. Expressions of cell mitotic regulators AuroraB and BubR1 were determined by immunohistochemistry. Results:Proliferation inhibition rates and apoptosis rates of HT-29 cells in 5-ASA 10,20,30,40 mmol/ L groups were increased with increase in concentration of 5-ASA(P ﹤ 0. 05). Proportions of cells in phase G0 / G1 in 5-ASA 30,40 mmol/ L groups were significantly lower than those in 5-ASA 0,10,20 mmol/ L groups (P ﹤ 0. 05);proportions of cells in phase S in 5-ASA 0,10,20,30 mmol/ L groups were significantly lower than that in 5-ASA 40 mmol/ L group(P ﹤ 0. 05);while proportions of cells in phase G2 / M in 5-ASA 30,40 mmol/ L groups were significantly higher than those in 5-ASA 0,10,20 mmol/ L groups(P ﹤ 0. 05). Mean optical density(MOD)values of AuroraB and BubR1 were decreased with increase in concentration of 5-ASA(P ﹤ 0. 05). Concentration of 5-ASA was positively correlated with proliferation inhibition rate and apoptosis rate of HT-29 cells(P ﹤ 0. 05),but was negatively correlated with MOD values of AuroraB and BubR1( P ﹤ 0. 05). MOD values of AuroraB and BubR1 were negatively correlated with proliferation inhibition rate and apoptosis rate of HT-29 cells as well as proportion of cells in phase G2/ M (P ﹤0. 05),but were positively correlated with proportion of cells in phase G0/ G1(P ﹤0. 05). MOD value of AuroraB was positively correlated with MOD value of BubR1(P ﹤ 0. 05). Conclusions:5-ASA can inhibit proliferation and induce apoptosis in HT-29 cells,the mechanism may be related to inhibiting expressions of AuroraB and BubR1 and the resulted effect on cell cycle.

This article was aimed to analyze literatures on preventive treatment of disease in acupuncture and moxi-bustion in order to understand its status and development trend. Bibliometrics and statistics method in NoteExpress were used in the analysis of related literatures about preventive treatment of disease in acupuncture and moxibustion from 1981 to 2013, which were included in CNKI. The analysis and research were from aspects such as year distri-bution, keyword, journal distribution, author distribution, and fund distribution. The results showed that there were 537 articles in total from 1981 to 2013. The number of literatures was in an overall growing trend.“Zusanli”,“Inner Canon of Huangdi”,“sub-health”, and“clinical research”were high frequency keywords. The journal distribu-tion conformed to the Brad Ford’s law. The author distribution conformed to the Lotka’s law. The number of fund papers was also increased. It was concluded that research on preventive treatment of disease in acupuncture and moxibustion was still in the period of rapid development and had not reached maturity. The preventive treatment of disease in acupuncture and moxibustion had broad application prospects. However, the research still required to be strengthened.

Objective To investigate the risk factors and prevention of the ERCP-related upper gastrointestinal bleeding in patients with choledocholithiasis.Methods The clinical data of 678 patients who received ERCP in Yijishan Hospital of Wannan Medical College between January 2014 and December 2014 were reviewed.Univariate and multivariate analyses were performed to define the independent risk factor(s) for delayed hemorrhage following ERCP.Results A total of 383 patients,in whom 157 were males and 226 were females,the age ranged from 19 to 88 years,were recruited to the study.ERCP-related upper gastrointestinal bleeding occurred in 41 patients(10.7％),intraoperative bleeding occurred in 27(7.0％) and postoperative hemorrhage in 14 (3.7％).Twenty-eight cases were mild bleeding,9 moderate (4 caused by laceration of the cardiac mucosa) and 4 were severe bleeding.All patients recovered and were discharged,and no death occurred.No single case required surgical conversion.Univariate analysis revealed that cholangitis,medical history of aspirin/clopidogrel,giant diverticulum and the major papilla inside the diverticulum were risk factors for delayed hemorrhage following ERCP (P ＜ 0.05).Multivariate analysis indicated that independent risk factors were associated with cholangitis (OR =4.125,95％ CI:1.306-13.031,P ＜ 0.05),medical history of aspirin/clopidogrel (OR =10.220,95％ CI:2.997-34.853,P ＜ 0.01) and major papilla occurred inside the diverticulum(OR =14.064,95％ CI:1.888-104.762,P ＜ 0.05).Conclusion Cholangitis,medical history of aspirin/clopidogrel and major papilla at the diverticulum may increase the risks for upper gastrointestinal bleeding in ERCP in patients with choledocholithiasis.However,the hemorrhage should be actively managed,especially in endoscopy,once the bleeding occurs.

Objective To investigate the current situation of psychological capital and job burnout of psychiatric nurses and explore the relationship between psychological capital and job burnout.Methods There were 142 psychiatric nurses collected in the study by convenience sampling.The Psychiatric Capital Questionnaire (PCQ) and Nurse Burnout Scale (NBS) were used to measure the level of psychiatric capital and job burnout of psychiatric nurses.The Pearson correlation analysis was used to explore the relationship between psychological capital and job burnout.Results The psychological capital of psychiatric nurses had a negative correlation with job burnout; the higher of the nurses' psychological capital,the lower their sense of job burnout,vice versa.Conclusions In order to improve nursing quality,nursing managers can reduce the job burnout by improving the psychological capital of psychiatric nurses.

Objective To observe if IL-2 gene can express in hepatic carcinoma cells by double targeting of hepatitis B virus envelope (HBVE) and alpha fetoprotein (AFP) promoter.Methods HepG2 cells,L02 cells,and HepG2.2.15 cells were cultured in vitro.HBVE was obtained by PEG8000 concentration assay,and the acquired HBVE was used to pack recombintional gene.Human AFP promoter-IL-2 recombinational gene was obtained by PCR.Then HepG2 cells and L02 cells were transfected by transient transfection and the expression of IL-2 was detected by RT-PCR and Western blot.Results IL-2 was detected in HepG2 cells by RT-PCR and Western blot but not in L02 cells.Conclusion By using HBVE as a gene transporter,human AFP promoter-IL-2 recombinational gene can express in hepatic carcinoma cells,thereby it can increase the safety of exogenous gene transfection of hepatic carcinoma cells.

BACKGROUND:Anterior decompression, bone graft fusion, plate and screw fixation are commonly used in clinic for the treatment of thoracolumbar burst or comminuted fractures. The posterior surgery including decompression laminectomy and pedicle screw internal fixation.
OBJECTIVE:To explore the biomechanical changes of spine after thoracolumbar fracture and dislocation treated with pedicle screw internal fixation.
METHODS:Forty-six patients with thoracolumbar fracture and dislocation were treated with pedicle screw internal fixation, and then the recovery of spinal cord injury and fracture reduction were observed after internal fixation, immediately after internal fixation and 1-year fol ow-up.
RESULTS AND CONCLUSION:Al the 46 cases were fol owed-up. Frankel function classification assessment was used to evaluate the spinal cord injury grade. There was no significant difference in the percentage of A grade between periods (P>0.05), and there were significant differences in the percentage of B-E grades when compared between immediately postoperative period, 1-year fol ow-up period and preoperative period (P<0.05), but there was no significant difference when compared between immediately postoperative period and 1-year-fol ow period (P>0.05). The anterior height percentage, posterior height percentage and Cobb angle indicators were used to assess the fracture reduction, and the results showed there was no significant difference in posterior height between periods (P>0.05), and there was significant difference in Cobb angle when compared between immediately postoperative period, 1-year fol ow-up period and preoperative period (P<0.05), but there was no significant difference between immediately postoperative period and 1-year fol ow-up period (P>0.05). The results indicate that pedicle screw internal fixation system for the treatment of thoracolumbar spinal fracture and dislocation is conducive to the fracture reduction and functional recovery.

Objective To observe the clinical efficacy of Shenfuyishen Capsule in the treatment of diabetic nephropathy of yangxuxueyu type in high- altitude regions.Methods We divided 53 patients randomly into two groups:31 in the observation group and 22 in the control group.Patients in the control group were treated with conventional regime of Western Medicine.Those in treatment group were given extra treatment with Shenfuyishen Capsule.Then we tested serum creatinine (Scr ),uric acid (UA),urinary microalbumin (MALB )and GFR. Results The total efficiency was 90.32% and 81.82% in the treatment group and the control group,which was significantly different (P < 0.05 ).Major symptoms improved after treatment in both groups,which differed significantly compared with those before treatment (P <0.05).ALT in the treatment group was not different after treatment.CR,UA,MALB and GFR significantly differed before and after treatment in both groups,especially in the treatment group (P <0.05).Conclusion Shenfuyishen Capsule helps improve major clinical symptoms and decrease CR,GFR,UA and MLAB levels in patients with diabetic nephrology,thus protecting renal function and delaying the process of diabetic nephropathy.

BACKGROUND:Oral insulin iS easy to be degradated by hydrochloric acid in gastric iuice and the enzyme which exists in stomach and intestines,while it is dimcult to through the epithelium cell membrane of gastrointestinal.Even a lasting-long injection iS taken,and it still needs an injection once 36 hours.OBJECTIVE:To prepare oraI insulin sustained-release microspheres.DESIGN,TIME AND SETTING:Contrast observation which was carried out at the Laboratory of Medical Chemistry of Nanjing Information Engineering University from September 2003 to December 2004.MATERIALS:Insulin Was provided bv Nanjing Medicinal Factory of Bio-Chemistry.METHODS:A new method tO prepare the microsphere,I.e.,drying through liquid phase was adopted with the special pH and the acrylic acid polymer which could be dissolved as the medicine'S carrier of the sustained-release microphere.MAIN 0UTCOME MEASURES:The effects of polymer concentration and agitation rates on size of microspheres and drug incorporation efficiency were studied by scanning electronic microscopy(SEM)and ultraviolet-spectrophotometry.RESULTS:The temperature,drug payloads and the content of emulsifier played important effects on microspheres formation.The mean diameter of microspheres decreased with increasing in stirring rate and decreasing in polymer concentration,and drug loading efficiency increased with increasing in stirring rate.CONCLUSION:Insulin-loaded polyacrylate sustained-release microspheres is prepared preferably.

ObjectiveTo explore the diagnosis and treatment of incomplete Kawasaki disease.MethodsA retrospective analysis was applied in 39 children patients clinically diagnosed as Kawasaki disease.ResultsThirtynine cases were found to have a high temperature over 5 days( 100％ ),30 patients with bilateral conjunctival congestion (76.9％),28 cases with cherry pink and chapped lips( 71.8％ ),21 patients with erythema multiforme and erythra(53.8％ ),20 cases with hand-foot scleredema(51.3％ )and 10 patients with cervical lymphatic enlargement (25.6％).In addition to the high temperature,22 cases were found to have three classic clinical diagnostic characterstics(56.4％ ),and 17 patients with two classic characterstics(43.6％ ).During the course of disease,C-reactve protein was observed to increase,and erythrocyte sedimentation rate speed up significantly.Intravenous infusion of gamma globulin and oral administration of aspirin was confirmed to be effective for incomplete Kawasaki disease.ConclusionThe careful observation and complete clinical examination was conducive to early diagnosis of incomplete Kawasaki disease instead of misjudge and missed diagnosis.