OBJECTIVE To investigate drug-resistance status of Klebsiella pneumoniae(KPN) in nosocomial infections and provide the reference for rational application of antibiotics.METHODS According to Nationwide Rules of Clincal Laboratory Manipulation,the strains were identified and drug-sensitivity test was performed by K-B methods.The extended-spectrum-?-lactamases(ESBLs) producing strains were detected by double-disc synergy test and disc confirmed test.RESULTS Totally 356 strains of KPN were mainly isolated from respiratory department,gerontology department,neurosurgery department and chest surgery department.The specimens were mostly sputum,pharynx swab,urine and secretion.The detection rate of ESBLs-producing KPN was 36.0%.The drug-resistance rate to common antibiotics in ESBLs producers was generally higher than that in ESBLs nonproducers.No KPN was resistant to imipenem and meropenem.CONCLUSIONS Drug-resistance status of K.pneumoniae in nosocomial infections is very serious.We should strengthen monitoring and controlling of it.

Objective To observe the expression of macrophage migration inhibitory factor (MIF) in colon tissues of rats with 2,4, 6-trinitrobenzene sulfonic acid (TNBS) induced colitis and the relationship between MIF and the severity of the colitis. Methods 24 Spra-gue-Dawley rats were randomly divided into three groups: normal group, model group Ⅰ and model group Ⅱ. Rat colitis model was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS), and disease activity index (DAI) was calculated every day. The myeloperoxidase (MPO) ac-tivity was detected by biochemistry, the expression of MIF in colon tissue was detected by semi-quantitative reverse transcriptional chain reac-tive (RT-PCR). Results In normal group, DAI, MPO activity and MIF mRNA level were 0.51±0. 28,0. 38±0. 18,0. 11±0. 03, while in model group Ⅰ were 5.04±0.73,1.68±0. 45,0. 65±0.04 and in model group Ⅱ were 8. 13±0.71,2. 70±0. 35,0. 81±0. 05. Com-pared with normal control, the expression of MIF mRNA significantly increased (P<0.05), MPO activity and DAI also significantly in-creased(P<0.05)in model group, especially in model group Ⅱ. Conclusion The expression of MIF increased in experimental colitis, and there was a positive relation between MIF expression and the severity of colitis, which suggested that MIF may be related to the pathogen-esis of TNBS-induced colitis.

0.05),and during which,no adverse drug reactions were observed.The prophylactic medication days,antibiotic drugs costs and total drugs costs in hospitalization were significantly lower in the trial group than in the control group(P

30% after ANH) and blood volume was maintained with crystalloid and colloid (1 : 3) . The preoperatively collected blood and the blood salvaged during operation were infused back to the patient after CPB. The operations were performed under CPB with moderate hypothermia. In patients undergoing atrial or ventricular septal defect tranexamic acid 20 ml-kg-1 was given and in other patients aprotinin 5 000 000 U was given. Hb, Hct and platelet count (PLT) were measured and recorded before operation, after ANH and 24h after operation. The volume of RBC salvaged during operation, CPB time and the volume of chest tube drainage at 24h after operation, the volume of whole blood, plasma, packed RBC, cryoprecipitate and platelet transfused and hospitalization time were also recorded. Results The demographic data were comparable between the two groups. There was no significant difference in CPB time and the total days of hospitalization between the two groups. The Hb at 24h after operation was significantly higher in group A +C [(122.2+18.8)g/L] than that in group C [(112.3+15.6)g/L] (P

Objective As a special type of chronic pancreatitis, autoimmune pancreatitis ( AIP ) is gradually being recognized?In 2001,researchers found that serum IgG4 levels were significantly increased in AIP patients,2% of chronic pancreatitis was 1?AIP; 1?AIP was considered a systemic disease associated with IgG4?So far,a number of autoimmune antigens associated with 1?AIP have been identified,but the role of IgG4 in the disease is certain,because the pathogenesis of the disease is not sure,the diagnosis and treatment of AIP is not optimistic.

Objective To objectively evaluated of the efficacy of bronchodilation agent by using pulmonary function .Methods 104 childrens with airway hyperresponsive diseases who saw doctor at the Guangdong Women and Children′s Hospital,aged 0~6 years.They were classified into three groups:salbutamol group(n=36);ipratropium bromide group(n=32) and two drugs group(n =36).Each group received salbutamol or ipratropium bromide or both.Then tidal breathing flow-volume loop and parameters were obtained before and after aerosol inhalation to evalu-ate the effects of drugs .Results There were significant improvement in each group after the inhalation of bronchodi -lators.Conclusion The two drugs of Salbutamol and ipratropium bromide can be used as the selective drugs of bron -chodilation .

Objective To investigate the clinical characteristics of Takayasu′s arteritis (TA) and the difference between male and female patients. Method The clinical features of 159 patients with TA were retrospectively analyzed. Results In our series of patients, the ratio of male and female was 1:3.1. The average age of male and female patients at onset of first symptom was (32?13) years and (24?9) years respectively (P

Objective:To survey the prevalence of internet addiction disorder(IAD)of vocational college freshmen and analyze its relationship to their childhood abuse and neglect.Methods:IAT,SCL-90 and CPANS were used to investigate 740 freshmen of Yongzhou Technical Vocational College.Results:The rate of IAD among 740 freshmen is 3.2%.The factor scores of IAD students were significantly higher than those of normal students(P

0.05).CONCLUSION:The mutagenesis of Leflunomide 3-methyl-isomer was not observed in these tests.

Objective To investigate the X-ray photographic and magnetic resonance imaging (MRI) features of hip pigmented villonodular synovitis (PVNS). Methods A retrospective analysis was carried out in 19 patients with hip PVNS confirmed by operation and pathological examination.The X-ray plain photography in 10 patients and MRI features in 19 patients (of which 9 received enhanced scan) were analyzed.Results (1)The results of X-ray photography were as follows:Of 10 patients,6 showed swollen joint capsule,2 had higher-density masses around the joint;joint space narrowing was found in all of the 10 patients;except 2 patients,8 showed various degrees of bone erosion in the margin area of the joint,in the intertrochanteric area and around neck ,and showed capsule-like clear focus area with sclerotic margin in the joint surface of the bone;bonespur was found in the edge of the joints of 4 patients,of which 3 were complicated with osteoporosis.(2)The manifestations of MRI features:of 19 patients with hip PVNS,17 were differentiated as the diffused and 2 as the focalized.All of 17 diffused hip PVNS showed different degrees of diffuse hyperplasia of synovium,5 of them had intra-articular effusion,13 showed multiple-diffused nodular synovial changes with low signals of T1WI and T2WI,14 showed different degrees of bony erosion and depression in acetabulum,in the head of the femur and around the neck,which signals were similar to those with synovial hyperplasia.Of the 14 patients,8 foci presented circular low signals,capsule-like clear images under the bony joint surface with medium to low signals of T1WI and T2WI,and diffused swelling close to medullar cavities with sheet-like high signals of T2WI/STIR.The results of contrast-enhanced scanning for 8 of the 14 patients showed enhanced signals in the joint synovium and focus area of joint bones.Two focalized PVNS patients showed single mass on the outer flank of the femoral neck with medium to low T1WI signal and with medium to high T2WI signal,and also presented multiple dot-like and nodule-like foci with low T1WI and T2WI signals.One of the 2 focalized PVNS patients showed arc-shaped bone erosion at the outer margin of the femur.Conclusion MRI features of hip pigmented villonodular synovitis exert specific characteristics,which are valuable for the diagosis of PVNS;X-ray plain photography has indicative value for the diagnosis,but it has limitation.