The study on aldose reductase gene intron 8 A95 C polymorphismin 209 type 2 dia-betic patients and 84 healthy controls showed that the frequency of genotype AAis 7 %in DN,higherthan that in non-nephropathy (1 %) and control (1 %) ,suggsting that intron 8 genotype AA may be arisk factor for diabetic nephropathy .

AIM To study mechanism of endothelin (ET) on canine pulmonary veins. METHODS The isometric tension of pulmonary venous strips was recorded. RESULTS ET 3 and IRL1640 produced contraction in pulmonary venous strips. ET 3 induced contraction was markedly suppressed by BQ123 (P

Objective To construct Hepatitis B Virus (HBV) Chinese-strain wild type and Lamivudine-resistant variant recombinant baculovirus. Methods 1.2-unit length HBV construct (adr serotype, C genotype) was cloned into the multiple cloning region of the pFastBac Ⅰ vector and then transformed into DH10Bac, in which homologous recombination occurred between the pFastBac vector and the inside bacmid with the help of helper plasmid. So HBV recombinant bacmid was produced and transfected into sf9 cells to generate a Recombinant HBV baculovirus called vAcHBVc. The viral titers were detected by immune plaque assay and TCID 50. Lamivudine resistance mutation (YVDD) was introduced by site-directed mutagenesis using continuous PCR. HBV YVDD recombinant baculovirus (vAcHBVcYVDD) was generated and titered as above. Meanwhile, the recombinant HBV baculovirus containing GFP reporter was constructed for evaluating the efficacy of transfection and titration of virus. Results Recombinant wild and YMDD variant HBV baculovirus were successfully developed and the titer was between 10~6～10~8pfu/ml through plaque assay. Conclusion Different kinds of recombinant HBV baculovirus could be generated rapidly and efficiently with Bac to Bac expression system. The developed recombinant HBV baculovirus could be further used in the study of anti-viral resistances.

Objective:To study the expression of Survivin and p27～(kipl)and their relationship with the clinical features of gallbladder cancer.Methods:Fifty specimens of gallbladder cancer,20 specimens of chronic cholecystitis,20 specimens of polypoid lesions of gallbladder(PLG)were included,the expression of Survivin and p27～(kipl)were detected using immunohistochemistry assay.Results:In 50 cases of gallbladder cancer,39 cases expressed Survivin and 22 cases expressed p27～(kipl).There was a negative correlation between the expression of p27 and survivin(P0.05),but the expression of p27～(kipl)was related to the pathological grade,clinical stage and lymph node metastasis of gallbladder cancer(P

To observe the effects of polyclonal antibodies to rat LPS binding protein on LPS induced activation of NF ?B and production of TNF ? and IL 6 in the lung. The activation of NF ?B and the contents of TNF ? and IL 6 were measured by electrophoretic mobility shift assay and ELISA respectivly. We found that both the activity of NF ?B and the levels of TNF ? and IL 6 in the lung were significantly decreased by the antibodies when used at early stage, but not at late stage after LPS challenge. These data indicated that the antibodies to LPS binding protein might have a potential value in the prevention of acute lung injury as a result of LPS challenge.

While histamine plays an important role in the pathogenesis of allergic diseases, such as allergic rhinitis, H1-antihistamines, which have been using in the treatment of allergic diseases for more than 70 years, are considered as the cornerstone of the medication of allergic diseases. In this review, we discuss the history of histamine studies and anti-histamine discovery, the histamine receptors, as well as the mechanisms and the safety of H1-antihistamines.
Anti-Allergic Agents ; therapeutic use ; Histamine H1 Antagonists ; therapeutic use ; Humans ; Rhinitis, Allergic, Perennial ; drug therapy

OBJECTIVE:To establish an HPLC method for the determination of Tanshinone Ⅱ A in Huganning tablets.METHODS:The determination was performed by HPLC using C18 column(200 mm?4.6 mm,5 ?m) with the column temperature maintained at 25 ℃.The mobile phase consisted of methanol-water(80∶20) at a flow rate of 1.0 mL?min-1 and a UV detection wavelength of 270 nm.RESULTS: The calibration curve of Tanshinone Ⅱ A was in good linearity over the range of 4.999～44.999 ?g?mL-1(r=0.999 7) and the mean recovery was 99.07%(RSD=0.33%,n=9).CONCLUSION: The method is sensitive,accurate and reproducible,and it can be used for the quality control of Huganning tablets.

BACKGROUND:To prepare glucose-responsive microcapsules which can control insulin release as changing the glucose concentration in the medium is of great significance to control the occurrence and development of diabetes mel itus. OBJECTIVE:To study the performance of glucose-responsive alginate/modified-chitosan/alginate microcapsules carryingβ-TC3 cells. METHODS:Glucose-responsive alginate/modified-chitosan/alginate microcapsules were prepared by layer-by-layer self-assembly method to evaluate the performance. And the glucose-responsive microcapsules carryingβ-TC3 cells were prepared to observe the cel proliferation within the microcapsules. RESULTS AND CONCLUSION:The integrity rate of glucose-responsive alginate/modified-chitosan/alginate microcapsules could be 95%after 48 hours oscil ation, and the hardness of microcapsules lowered, but the elasticity increased. The permeability test showed that microcapsules intercepted macromolecular substances such as bovine serum albumin and immuno-globulin G. The microcapsules could release more insulin with the increase of glucose concentration. As described above, the glucose-responsive alginate/modified-chitosan/alginate microcapsules had good mechanical strength, immunoisolation effect and glucose sensitivity. Theβ-TC3 cells entrapped in the glucose-responsive microcapsules could grow wel and the peak of cel proliferation lagged behind as compared with non-microencapsulated cells, indicating the glucose-responsive microcapsules had good biocompatibility.

Objective To investigate the expression of hMLH1 and hMSH2 in hilar cholangiocarcinoma and its relation to clinical features and prognosis of the tumor.Methods The expression of hMLH1 and hMSH2 was determined with immunohistochemistry in 54 specimens of hilar cholangiocarcinoma and 25 of normal bile duct tissue.Lahoratory data were then analyzed statistically together with the related clinicopathological data.Results 1)hMLH1 and hMSH2 were expressed in 24 and 21 out of the 54 cases of hilar cholangiocarcinoma(44.4%,38.9%)and 23 and 21 of the 25 normal cases(92%,84%),respectively(P＜0.05).2)The expression of hMLH1 and hMSH2 in hilar cholangiocarcinoma had no association with the age,gender,tumor size and Bismuth type(P＞0.05)but close relation to lymph node metastasis and pathological changes(P＜0.05).3)The 2-year survival rate was markedly lower in hMLH1-negative patients than in hMLH1-and hMSH2-positive ones (15%vs.45.4%,23.5%vs.44%,P＜0.05).Conclusions Joint action of hMLH1 and hMSH2plays an important role in the oncogenesis and metastasis of hilar cholangiocarcinoma.These two may be valuble factors to indicate prognosis of the tumor.

Biomedical Research ; China ; Foundations ; Otolaryngology