OBJECTIVE: To study the change of consumption of antibacterial agents especially the ceftazidime and its effect on drug resistance of Pseudomonas aeruginosa (PA). METHODS: The consumption of the top ten antimicrobial agents in terms of cases/times of medication from 2005 to 2006 in a hospital and its correlation with the drug resistance of Pseudomonas aeruginosa were analyzed. RESULTS: A linear correlation was noted between the consumption of ceftazidime and resistance of Pseudomonas aeruginosa to ceftazidime and another seven antimicrobial agents in the next quarter (P

OBJECTIVE:To evaluate the economics of alendronate sodium preventing osteoporotic fractures in postmenopausal women,and to provide reference for osteoporotic and osteopenia patients to select economical and effective therapy plan. METH-ODS:Using Markov economic model,TreeAge Pro 2011 software was used for cost-utility analysis using RCTs literature data of basic medication(calcium and vitamin D3)and basic medication combined with alendronate sodium as baseline data. Using quality adjusted life years(QALYs)and incremental cost utility ratio(ICUR)as economics evaluation indexes,the stability of established model was investigate by single factor sensitivity analysis. RESULTS:QALYs of the basic treatment group was 0.704 years,and QALYs of drug combination group was only 0.708 years. However,ICUR of two groups reached 714252.44. For the late period of hip fracture and other fractures,drug combination group was in high cost and low benefit,obviously had no economic advan-tage. For vertebral fractures,the cumulative probability of fracture in drug combination group was significantly lower than basic treatment group,and ICUR of the two groups was only 13902.17,with economic advantage. For hip fracture,the cumulative prob-ability of fracture in basic treatment group was the same as drug combination group,and ICUR was 19109.00,with economic ad-vantage. For wrist fractures,curative effect and economy needed further study. CONCLUSIONS:For the low-risk population with osteoporotic fractures,calcium and vitamin D3 are more economical in preventing osteoporosis fractures. For the high-risk popula-tion with vertebral and hip fractures,alendronate sodium combined with basic treatment is effective and economical.

Objective To investigate the possibility of chest approach gasless endoscopic surgery for the parathy-roid tumor. Methods Through a 2 cm incision on the surface of chest bone, a gasless procedure was developed to management of parathyroid tumor in our department. There were 2 cases of the parathyroid cyst-adenoma were dis-sected. Results 2 cases were successfully performed, and there were no paralyze of the revers laryngeal nerve and hypofunction of parathyroid could be observed. The cosmic and mini-invasive results were obtained. Conclusion Chest approach gasless endoscopic surgery for parathyroid tumor is a safe, mini-invasive, and cosmic procedure for selected patients.

OBJECTIVE To construct the lentiviral vector system expressing MDR1 small interference RNA, and identify reversal efficiency of multidrug resistant phenotype in the human laryngeal cancer multidrug resistance cell lines (LSC-1/TAX). METHODS Three target sequences of oligonucleotides were selected according to MDR-1 gene sequence, the complementary DNA contained both sense and antisense strands were designed and synthesized. After the oligonucleotides were inserted into the plasmid expression system pLVTHM, the plasmid was cotransfected along with pCMV-dR8.74 and pMD2G into 293T cell lines to package lentiviral particles. Interference efficiency of the lentiviral vector system expressing MDR1 small interference RNA was determined by RT-PCR, real time PCR and Western Blot in the human laryngeal cancer multidrug resistance cell lines (LSC-1/TAX), drug resistance was measured by MTT assay after interference. RESULTS It was confirmed by digestion and sequencing that lentiviral vector had the correct structure and could express the GFP and siRNA. The functional titer of concentrated virus was more than 1?108TU/ml. The vectors expressing 3 target sequences can infect LSC-1/TAX, and the third vector has the best interference efficiency. CONCLUSION The lentiviral vector system expres-sing MDR1 siRNA has been constructed, which is necessary to reverse multidrug resistance phenotype in the human laryngeal cancer multidrug resistance cell lines

OBJECTIVE To study the results of the reoperation for thyroid cancer. METHODS The clinical data of 288 cases who underwent reoperation for thyroid cancer were analyzed retrospectively. There were 69 male and 219 female. There were 249 cases (86.5 %) with papillary adenocarcinoma, 27 cases(9.4 %) with follicle adenocarcinoma, 6 cases (2 %) with medullar cancer, and 6 cases (2 %) with anaplastic cancer pathologically. The types of operation were lobectomy and near total lobectomy. The unilateral or bilateral neck dissections were performed in selected cases. RESULTS Pathological examination reveal that there were 63 cases of cancer residual in the ipsilateral side and 33 cases of the opposite side. The cervical node metastasis were present in 164 cases of patients at the same side. CONCLUSION The ipsilateral side lobaectomy and/or contralateral side near total lobectomy should be employed for the thyroid cancer patients. The neck dissection should be performed in most of the patients.

OBJECTIVE To study the application of transcervical approach for benign parapharyngeal space tumors.METHODS The clinical data of 19 patients(20 operations) with benign parapharyngeal space tumors were analyzed retrospectively.RESULTS The 19 patients with benign parapharyngeal tumors were operated on in Tongren Hospital from July 2004 to July 2007.All patients were operated on through trans-cervical approach under general anesthesia.They were followed up from 6 to 36 months.Recurrence was found in a girl 15 months after operation,and was reoperated on by the same approach.No complications occurred.CONCLUSION The patients with parapharyngeal space tumors must be carefully studied by CT or/and MRI scan.If a clear tumor capsule is identified,the tumor can be removed through transcervical approach.Transcervical approach is a useful approach with least damage,and nearly all benign tumors of parapharyngeal space can be removed through it.

Poly (hydroxyethyl methacrylate) (PHEMA) hydrogel for intraocular lens (IOL) materials was synthesized by solution polymerization using 2-hydroxyethyl methacrylate (HEMA) as raw material, ammonium persulfate and sodium pyrosulfite (KPS/SMBS) as catalyst, and trietyleneglycole dimethacrylate (TEGDMA) as cross-linking additive. Effects of reaction time, temperature, dosage of catalyst and cross-linking additive on mechanical strength and the equilibrium water content (EWC) of the PHEMA hydrogel were systematically investigated and their structure and optical property were also characterized. The experimental results showed that the optimum conditions for preparing PHEMA hydrogel are: catalyst 0.5 wt%, cross-linking additive 1.0 wt%, reaction temperature 40 degrees C, reaction time 36 h. Under the optimum conditions, the tensile strength of PHEMA hydrogel prepared is as high as 0.57 MPa, hardness of Shore A is 23.0, EWC is over 40%, and light transmittance is over 97%.
Biocompatible Materials ; chemistry ; Humans ; Hydrogel, Polyethylene Glycol Dimethacrylate ; chemistry ; Lenses, Intraocular ; Polyhydroxyethyl Methacrylate ; chemical synthesis ; chemistry ; Tensile Strength

In this paper, poly(D,L-lactide) (PDLLA), MDI chain-extending poly(D,L-lactide) (PDLLA/MDI) and MDI chain-extending poly(D,L-lactide)/hydroxyapatite composite (PDLLA/HA/MDI) were prepared respectively and the effects of moulding and extruding conditions on their mechanical properties were also investigated. At the optimal conditions, bending strength of PDLLA and PDLLA/MDI is 35.1 MPa and 51.3 MPa, respectively, and their bending modulus is 2413.6 MPa and 1830.9 MPa, respectively. Bending strength of PDLLA/HA and PDLLA/HA/MDI is 31.2 MPa and 55.4 MPa, respectively, and their bending modulus is 1735.0 MPa and 2068.5 MPa, respectively. These results have shown that the mechanical properties of PDLLA/MDI and PDLLA/HA/MDI have enhanced significantly by MDI chain-extending.
Biocompatible Materials ; chemistry ; Durapatite ; chemistry ; Mechanics ; Medical Laboratory Science ; instrumentation ; methods ; Polyesters ; chemistry

OBJECTIVE: To study the cytotoxicity of recombinate toxin MSH-Ang to Hep-2. METHOD: The depurated MSH-Ang were applied in cytotoxicity experiment, and the growth inhibiting action to laryngeal carcinoma cell Hep-2 were observed. RESULT: Recombination protein inhibited the growth of laryngeal carcinoma cell Hep-2, and its inhibiting action enhanced and corpuscular mortality rate increased along with the concentration increasing. CONCLUSION Recombinant toxin MSH-Ang can not only take special effect in tumors with high MSHR, but also target to many other popular tumors.
Angiopoietins ; genetics ; pharmacology ; Cell Line, Tumor ; Genetic Engineering ; Humans ; Laryngeal Neoplasms ; Melanocyte-Stimulating Hormones ; genetics ; pharmacology ; Recombination, Genetic