1.Evaluation of Direct Rapid Immunohistochemical test (DRIT) of Canis lupus familiaris hippocampal touch impression smears using a monospecific polyclonal antibody for rabies virus detection.
Ang C. Mary Jasmin ; Llenaresas-Manalo Daria ; Jarilla Blanca ; Tuason Laarni ; Avenido Eleonor ; Demetria Catalino ; Medina Plebeian ; Quaimbao Beatriz ; Acosta Luz ; Inoue Satoshi ; Estacio Ma. Amelita
Acta Medica Philippina 2016;50(2):51-55
BACKGROUND: Rabies is an important zoonotic disease that needs to be eradicated worldwide. It is still prevalent in the Philippines, thus development of a relatively affordable but still accurate and rapid post-mortem detection test for the virus is desired, especially in regional laboratories.
METHODS:The study evaluated the Direct Rapid Immunohistochemical Testing (DRIT) of hippocampal touch impressions of suspected rabid Canis lupus familiaris using monospecific N protein polyclonal antibody developed by the Research Institute for Tropical Medicine (RITM). One hundred sixty (160) acetone-fixed hippocampal touch impressions were subjected DRIT.
RESULTS: One hundred thirteen (70.6%) out of 160 samples tested positive for rabies viral antigen (RVA) and 47 (29.4%) out of 160 samples tested negative for RVA. No false positive and false negative results were obtained. The results agree with the gold standard, dFAT.
CONCLUSION: DRIT was able to detect low to high concentrations of RVA in the hippocampal touch impressions based on the grading distribution. DRIT had 100% sensitivity, specificity and over-all accuracy using monospecific polyclonal antibodies, which suggests its use as a more affordable alternative to the gold standard dFAT.
Animal ; Antigens, Viral ; Dogs ; Hippocampus ; Rabies ; Rabies Virus ; Sensitivity And Specificity ; Touch ; Tropical Medicine ; Immunohistochemistry
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