In the study, X—ray, patholoSy and electron neuro—physiology were used for obsering the injury of facial nerve. The result revealed that the microstructure of the facial nerve was damaged while the macrostructure unchansed. The microstructure changes included: fibre broken—up, bleeding in perineurium, deformation of axon, mitochondria swelling. Nerve evoking potential examination indicated that the latency was prolonsed, amplitude decreased and nerve conduction velocity was delayed. This results showed that there existed indired nerve injury. It is important to relieve the intraneural pressure, recover microcirculation and remove heamatoma in muscle and fascia in early stage

Objective: To view the characteristics and the primary treatment of civilian firearm wound in the maxillofacial region.Methods: 301 cases of the casualties admitted to our hospital were retrospectively investigated,the epidemic, etiology, wound characteristics, and treatments of the patients were viewed respectively. Results: Of all the cases, the ratio of male to female was 5.1∶1.0. 57% of the injuries were caused by gunshot, 66% were with multiple injuries.16% combination wound were craniocerebral injuries. In this group of patients, more than 900 wound tracks were found, 72% of the tracks were nonpenetrating and 67% of those were with foreign bodies lodged in. Burns with blast injuries were the main complex wounds, which mostly occurred in explosion. The treatments mainly included life maintaining, earlier debridement and infection control. Conclusions: The maxillofacial firearm injuries are complex and have their own characteristics depending on the firearms, the wound styles and the regional anatomy. All the factors must be considered when treatment is given.

objective: To study the effects of hydroxycampothecin(HCPT), pingyangmycin(PYM), cis platinum (DDP) or their combination on the proliferation of human tongue carcinoma Tca8113 cells. Methods: MTT assay, cell counting,clonogenic assay and HE staining were used to study the effects of HCPT, PYM, DDP or their combination on the proliferation of human tongue carcinoma Tca8113 cells. Results: ①Synergistic inhibiton was observed when HCPT at the concentration (ng/ml) of 10～100 combined with PYM at 32～320 or DDP at 32～320, antagonistic effect was observed when PYM at 32～320 combined with DDP at 32～320; ②After treatment with PYM, HCPT or PYM plus HCPT at IC 30 the clonogenecity (%) of the cells was 15.2, 11.6 and 4.1 respectively, while that of untreated cells 31.7. Conclusion: The combination of HCPT with PYM may be synergistic in the inhibition of tongue carcinoma cell proliferation.

Objective: To establish an efficient animal model for the study of explosive injury in maxillofacial region.Methods: The detonator, steel spheres as fragments simulants shot by smooth-bore barrel and a synchronizer were used to define the wound model in the maxillofacial region of 18 dogs. The whole system was triggered when shooting. Wounding parameters and the changes of targets after explosion were observed and recorded. Results: The overpressure of blast wave decreased exponentially according to the distance from explosive. After injury, the local damage of dogs was serious and extensive compounded with the damages in heart, lung and brain. The injury was aggravated with the increasing of over pressure and energy absorption. Conclusion: The new wound model can be used for the study of explosive injury in maxillofacial region that is characterized by serious tissue damage and multi-etiology.

Recombinant human epidermal growth factor (rhEGF, 20 ng/ml) and Genistein (740 μmol/ml) were added to the cells from 12 cases of human pituitary tumors and co-cultured in vitro. The 3H-thymidine uptake of tumor cells was measured by scintillation counter and tyrosine kinases activity was detected by using immunocytochemical method. The results showed that 3H-thymidine uptake and tyrosine kinases activity were increased in 10 out of 12 cases of human pituitary tumor by EGF and both declined by Genistein. It was suggested that EGF had a strong mitogenic effect on majority of pituitary tumors. Genistein could abolish the effects of EGF promoting the proliferation of the tumor cells, inhibit the growth of the tumor and might be useful in pituitary tumor treatment.

Rabbit osteoblasts were isolated, expanded in vitro, and then seeded into coral-hydroxylapatite. The osteoblasts/coral-hydroxylapatite composite was were implanted into the muscles of rabbits′ back. Bone regeneration was observed 4, 8 weeks after implantation by histological and scanning electron microscopic examinations.The results showed that osteoblasts could adhere and proliferate well on the surface of coral-hydroxylapatite. 4 weeks after implantation osteoid tissue was observed, 8 weeks after implantation large amount of new bone was found. It suggested that osteoblasts/coral-hydroxylapatite composite may be used for bone tissue engineering.

Objective:To study the effects of hydroxycamptothecine(HC PT ) and pingyangmycin(PYM) alone or in combination(HCPT/PYM) on the proliferation and telomerase activity of human tongue carcinoma Tca 8113 cells.Methods :The growth inhibitary effects of HCPT or PYM on Tca 8113 cells were stu died by MTT assay and the IC 30 values of the two drugs were obtained. Then the cells were exposed to HCPT or PYM at IC 30 , or to their combination. T he clonogenecity, cell cycle distribution, morphological change and telomerase a ctivity were studied by clonogenic assay, flow cytometry,transmision electron mi croscopy and telomeric repeat amplification protocol (TRAP) respectively.Results:IC 30 (ng/ml) of HCPT and PYM was 109 and 416 respectivel y, when in combination(HCPT/PYM) the IC 30 of HCPT and PYM was 35 and 100 r espectively. The clonogenecity(%) of the control, HCPT,PYM and HCPT/PYM treated cells was 31.7,11.6,15.2 and 4.1 respectively. PYM decreased S and G 2 phase ce lls,increased G 1 phase cells.HCPT or HCPT/PYM decreased G 1 phase cells and i ncreased S and G 2 phase cells.Drug treatment resulted in cell organ degenerati on and decrese of telomerase activity.The A value at A 450 of telomerase in control,HCPT,PYM and HCPT/PYM treated cells was 1.89?0.03,0.82?0.02,0.77?0.0 2 and 0.53?0.02 respectively(P

Objective:To explore the mechanism of growth factor autocrine stimulation in human pituitary tumors. Method: Immunohistochemical analyses of EGF、TGF-αand EGFR were studied on paraffin-embeded sections of 30 pituitary tumors. Results: Expression of EGFR and its ligand EGF、TGF-αhas a variable stained cells intensity,density and type. EGFR and the ligands expressed in majority of pituitary tumors.Conclusion:The EGF autocrine stimulation exists in pituitary tumors,and tyrosine kinases inhibitors may be useful for pituitary tumors treatment.

Objective:To investigate the causes, characteristics and treatment of maxillofacial trauma in children. Methods: A retrospective analysis of 470 consecutive maxillofacial records of the patients not older than 14 years was conducted. Data regarding age, gender, cause, anatomic site and treatment were reviewed. Results:Most of the patients were mals(335 cases, 71.3% ), with a male and female ratio of approximately 2. 5;1 of the injuries, 28. 5% were due to accidental falls, 17.0% traffic accidents and 10.0% sharp implementt cutting. Injuries of soft tissue often occurred on gingiva, cheek, lip and chin. Mandibuir fratures were the most common (55.1% ) of all bone fractures. Conservative therapy, such as closed reduction surgery, maxillomandibular fixation, was usually performed. Conclusion: ①Boys are more tendent to be victims of maxillofacial trauma than girls. ②Falls are the first cause of child victims. ③Gingiva, chin, lip, check and mandible are the most commonly injuried sites.

Objective: To develop injectable tissue-engineered bone. Methods: Bone marrow cells isolated from lilac bone of New Zealand rabbits were cultured and induced to differentiate Into osteoblasts. The osteoblasts were mixed with 20 g/L alginate sodium solution to generate osteoblasts/alginate composite with final cellular density of 5 ? 10 6/ml. Calcium chloride was used as cross-linking agent. The osteoblasts/alginate composite was injected into the dorsal subcutaneous tissue of nude mice. The injected material with surounding tissue were examined with X-ray and histopathologic technique. Results: Four and eight weeks after injection, the hard knobbles were easily palpated under the dorsal skin of the animals. On X-ray photograph the knobbles showed calcified tissue image. In histological analysis, new bone formation was observed in the osteoblasts/alginate composite. The osteogenesis was in association with regenerated hematopoietic bone marrow. Conclusion: New bone tissue can be created through the injection of alginate sodium mixed with marrow stromal osteoblasts.