[Objective]To introduce the effect of reversed sural neurofasciocutaneous flap with muscle repairing the soft tissue defect of foot and ankle.[Method]Reversed sural neurofasciocutaneous flap with muscle was applied for repairing the soft tissue defect simultaneous open fracture of tibia lower section 6 cases,the soft tissue defect simultaneous chronic osteomyelitis of tibia lower section 5 case,the soft tissue defect of sole of foot simultaneous calcaneus epicarp 3 case, the soft tissue defect of sole of foot simultaneous calcaneus osteomyelitis or dead space 2 case. The scope of the flap was 18 c m?13 cm～11 c m?7 c m and that of the gastrocnemius flap was 4 cm?3 cm～9 c m?6 cm,the thickness was 1～3 cm,the skin flap was bigger than the muscle flap.[Result]Muscle side of the muscle flap was errhysising actively and the skin flap was good circulation duing operation, the muscle flaps were used clinically in 16 patiens all survived completely after operation. All cases were followed up from 5 to 17 months ,incision of open fracture healed primarily, osteomyelitis without reoccurring, the muscle flaps were satisfactory in appearance,feel recovered and walk well.[Conclusion]Reversed sural neurofasciocutaneous flap with muscle has sufficient blood supply and high survival rate,so it is an effective and feasible method in repairing the soft tissue defect of foot, ankle and tibia lower section in special cases.

OBJECTIVE:To establish a method for simultaneous determination of the contents of three components in synthesis and actual satongfeng injection.METHODS:A flexible tolerance simplex method was used for multi-wavelength spectrophotometry with interval of 1nm in 258～290nm wavelenth,33 wavelenths were selected.RESULTS:The linear range and average recoveries rate of sodium salicylate,phenazone and caffeine were 0～42,0～12,0～6?g?mL-1 and(99.93?0.22)%,(99.85?0.24)% and(100.19?0.40)% respectively.All were agreed with those by standard method.CONCLUSIONS:This method is easy in operation,fast and reproducible,applicable to automatic analysis,and therefore can be used for the quality control of the preparation.

Objective To evaluate the prognosis of recurrent laryngeal cancer after surgieat treatment, the study was designed to investigate the relationship of VEGF and Cath-D expression in patients with recurrent laryngeal carcinoma. Methods Five hundred and seventy-four cases of laryngeal carcinoma treated in our department from 1958 to 1999 were retrospectively reviewed. All patients were observed for 3 years postoperatively. The whole set of tissue microarrays prepared with tumor tissue from 574 patients, including recurrent tumor tissue from 56 patients, and also 39 normal laryngeal tissue from normal subjects. The tissue chips were analyzed by the SABC immunohistochemical method. Results VEGF and Cath-D were expressed in most of the laryngeal carcinoma and normal laryngeal tissue, but the positive ratio of them was different. There was higher expression of VEGF and Cath-D in recurrent laryngeal carcinoma compared with that in laryngeal carcinoma without recurrence and normal laryngeal tissue (P

Objective To evaluate the prognosis of laryngeal cancer in terms of postoperative recurrence,the study was designed to investigate the relationship of E-cadherin,CD44,and Tenascin expression in patients with recurrent laryngeal carcinoma. Methods 574 cases of laryngeal carcinoma treated in our department from 1958 to 1999 were retrospectively reviewed. All patients were observed for 3 years postoperatively. The whole set of tissue microarray contained tumor tissue from all these patients,including recurrent tumor tissue from 56 patients,39 normal laryngeal tissue from normal larynx. The tissue chips were analyzed by the SABC immunohistochemical method. Results E-cadherin,CD44,and Tenascin were expressed in most of the laryngeal carcinoma and normal laryngeal tissue,but the positive ratio was different. There was lower expression of E-cadherin in recurrent laryngeal carcinoma compared to that in laryngeal carcinoma without recurrence,and the difference was statistically significant ( P

Objective To evaluate the prognosis of laryngeal cancer postoperative recurrence, and to investigate the relationship of Cyclin D1, PCNA, and Ki 67 protein expression in patients with recurrent laryngeal carcinoma. Methods 574 cases of laryngeal carcinoma from 1958 to 1999 were retrospectively reviewed. All patients were follow up surveyed for 3 years postoperatively. The whole set of tissue microarrays from 574 patients contained tumor tissue, including recurrent tumor tissue from 56 patients, and 39 normal laryngeal tissue specimens from normal laryngeal were collected as control. The tissue chips were analyzed by the SABC immunohistochemical method. Results Cyclin D1, PCNA, and Ki 67 expressed in most of the laryngeal carcinoma and normal laryngeal tissues in a different positive ratio. There was higher expression of Cyclin D1, PCNA, and Ki 67 in recurrent laryngeal carcinoma compared to that in laryngeal carcinoma without recurrence and normal laryngeal tissue ( P

0 05) , RCR and RFER were more higher , RICR and RFIR were greatly lower in analgesia group than those in control group 3d after operation(P0 05), RCR increased markedly 7d after operation(P0 05) Conclusions Postoperative analgesia can help stabilize erythrocyte immunity function

Objective To investigate the effective method of the treatment of the infective, complex defect of skin and bone in crus. Methods On the basis of through anti-infection, the infective surface of wound with bone and skin complex defect was completely debrided. The infective and necrotic skin and bone were moved away, and the wound surface was covered with flaps having abundant circulation. The anti-infection therapy was continued after operation. When the infection healed and the normal skin covered the wound, the bone defect was renovated at the second stage. Result The transplanted flaps all survived and the skin covering the wound remained normal. After the follow-up of four to five years, all the transplanted bones healed completely. No limbs were amputured because of wound infection or the complex defect of bone and skin. Conclusion After the infective surface of wound was completely debrided, the skin defect was renovated with flaps having abundant circulation. On the basis of infection being totally cured, the lone-piece-bone defect was renovated. This method can maintain the limbs and creat conditon for the functional recovery.

Objective To investigate the effects of radiotherapy combined with resveratrol( Res)on proliferation, invasion and apoptosis of hepatoma cell line Bel-7404. Methods Bel-7404 cell line was treated with Res(25 μmol·L-1 ) combined with radiotherapy,then divided into four groups( group 1:the control;group 2:radiation at dose of 2 Gy;group 3:radiation at dose of 4 Gy;group 4:radiation at dose of 6 Gy). Cell proliferation and invasion were detected by MTT assay. Cell apoptosis were observed by fluorescence microscopy. Expressions of MMP-2 and VEGF proteins were determined by Western blotting. Results Compared with the control group,cell proliferation and invasion were significantly inhibited,while cell apoptosis was increased in all radiation groups(P〈0. 05). Conclusion The sensitivity of hepatoma Bel-7404 cells to radiation can be enhanced by resveratrol. Radiation therapy combined with resveratrol can inhibit proliferation and invasion of hepatoma cells and increase the cell apoptosis,which may be related with the down-regulation of MMP-2 and VEGF proteins.

From October 1985 to April 1989, free flaps using post tibial vessel of the healthy leg as a bridgework were applied to 7 cases of extensive traumatic wounds, including damage of main vessels, exposure of bone and large soft tissue defect, of the lower limbs. Success was obtained in all cases. The operative method, indication, selection of the skin donor area and the key factor of operative success were introduced and discussed in the article.

Objective To survey the quality of life and mental health status of pre-post household service workers who held a high school graduation certificate or above and had been engaged in different work.Methods A total of 254 household service workers completed Symptom Checklist 90 (SCL-90) and World Health Organization Quality of Life-Brief (WHOQL-BREF).Statistical analyses of variance and correlation were carried out to evaluate mental health status and quality of life of the participants.Results Abnormal score of SCL-90 was found in 8％ of household service workers who had graduated from high school.The psychological score of household service workers who had worked as workers(60.59± 2.18)was lower than those who had worked as service workers (66.23 ± 12.13) or peasants (65.40 ± 12.24) (P ＜0.05).Almost all factors of SCL-90 were negatively correlated with WHOQL-BREF (correlation coefficient -0.140 to-0.376).Conclusions Our data suggest that mental health status can be well in pre-post household service workers who held a high school graduation certificate or above.Special training should be given to the household service workers in accordance with their working experience.