OBJECTIVE To study the distribution and drug resistance of meticillin-resistante coagulase negative Staphylococcus(MRCNS) in neonate septicemia for rational use of antibiotics in clinic. METHODS The MRCNS isolates were detected by BD120 and ATB.Susceptibility test to 12 antibiotics was performed through disk diffusion test. RESULTS Totally 262 strains coagulase negative Staphylococcus(CNS) was isolated from 344 S.aureus strains.Among 262 CNS,there were 130 S.epidermidis strains(49.6%),94 S.hemolyticus strains(35.9%),21 S.hominis(8.01%),and 17 other kinds of Staphyloccus strains(6.49%).The isolated ratio of MRCNS was 65.0%(170/262).Susceptibility test showed that the MRCNS were multiple resistant. CONCLUSIONS CNS occupys the first place in infection of neonatal blood.The isolated ratio of MRCNS is high and shows multi-drug resistance.Vancomycin and some other glycopeptide antibiotics are the first choice for MRCNS infections.

Objective To investigate the protective effect of berberine (BBR) against ionizing radiation injury in rats and its mechanism of action. Methods Sprague-Dawley rats were divided into seven groups: normal control group, 1-Gy radiation group, 1-Gy radiation plus low-dose BBR (50 mg/kg) group, 1-Gy radiation plus high-dose BBR (150 mg/kg) group, 3-Gy radiation group, 3-Gy radiation plus low-dose BBR (50 mg/kg) group, and 3-Gy radiation plus high-dose BBR (150 mg/kg) group. All the groups except the normal control group were exposed to external irradiation with a medical electron linear accelerator, followed by BBR administration by gavage for consecutive ten days. The serum levels of superoxide dismutase (SOD), reduced glutathione (GSH), and malondialdehyde (MDA) were measured by using the micromethod. The pathological changes of the bone marrow and small intestine were observed with HE staining. Results Compared with the normal control group, the radiation groups showed significantly increased MDA levels (P < 0.05), significantly decreased SOD and GSH levels (P < 0.05), and more severe pathological damage of the bone marrow and small intestine. Compared with the radiation groups, the BBR groups showed significantly decreased MDA levels (P < 0.05), significantly increased SOD and GSH levels (P < 0.05), and reduced pathological damage to the bone marrow and small intestine, which were more marked in the high-dose BBR group. Conclusion BBR has a certain protective effect against radiation injury in rats, which may be through increasing the activity of antioxidant substances, enhancing free radical clearance, and thereby alleviating free radicals-caused oxidative damage.