Background: Wound healing mechanisms still unclear. Wounds are physical injuries that result in an opening or breaking of the skin. Proper healing of wounds is essential for the restoration of disrupted anatomical continuity and disturbed functional status of the skin. This is a product of the integrated response of several cell types to injury. Wound healing is a complex process that results in the contraction and closure of the wound and restoration of a functional barrier.Goal: The purpose of this study was to determine burn wound healing activity of Calvacin. Materials and Methods:We used in study as colored reaction thin layer chromatography (TLC) and UV-spectrophotometer methods to determine active compounds in the three component drug "Calvacin".In research were inducted 20 healthy white mice and 40 rats. Investigation was based and implemented at scientific research laboratory of Traditional Medical Science Technology and Production Corporation and Institute of Veterinary medicine of pathological laboratory. The study protocol was approved by the Ethics Committee of the Ministry of Health.Results:Results of the phytochemical investigations showed that contained in the new drug "Calvacin" determined the presence of bioactive substances such as flavonoids, saponin, tannin, curcuminoids, organic acids and minerals. Substance was absorbed 25.7% in 40 grade ethanol, 27.1% in 70 grade, 22.2% in 96 grade and 19.6% in sterile water. We was established quality quantities of Calvacin in the drug amount summary flavonoids 1% and determined by UV spectrophotometer method. Proceeding from the absorption maximum of the substances analyzed the wave length of detection was determined as 370nm. In the result of mineral study of the "Calvacin", Ca^2.3%, P-6.17%, K-5.58%Burn wound healing time is control group 35±0.1 days, on 26±0.1 day animals of Calvacin group, but animals of Vishinski's ointment group 33±0.1 (p<0.05).Conclusions:1. The phytochemical investigations contained in the "Calvacin" drug determined the presence of bioactive substances such as flavonoids, saponin, tannin, curcuminoids, organic acids and minerals.2. Calvacin preparation has less toxic and significantly stimulate experimentally induced wound contract.

BACKGROUND. The Mongolian traditional medicine Anar-5 is excellent for weak digestion and helps with stomach irritation, loss of appetite, and resulting body weakness. Anar-5 blends punicagranatum, cinnamomum cassia presl, piper longum, cardamom and alpiniaofficinarum. We are establish an experimental animal of chronic gastritis to investigate the effect of traditional medicine Anar-5 on rats gastric mucosa. Methods: In this study, the protective effect preparation in sixty five healthy, male wistar rats were treated with intragastric administration of ammonia water 0.1%. To rats in three experiments for 2 week, 4 week, and 6wk, gastric tissues were examined histopathologically for atrophic changes and blood’s gastrin produced by preparation treatment. Results: After the treatment of animals blood’s gastrin was significantly different from that in control group (p<0.05), and the gastric mucosal inflammation was infiltration of inflammatory cells, decreased thickness of lamina propria. Conclusion: Treatment with preparation from Anar-5 protectived by the chronic gastritis and gastric atrophy.

Background: In traditional medicine, the Shimshin-6 formulation, which consists of Rheum undulatum L., Hippophae rhamnoides L., Zingiber officinalie Roscoe, Saussurea Lappa C.B.Clark, Sal ammoniacum, Tronae veneni, is recommended for women experiencing menstrual retention disorders. In recent years, Shimshin-6 has been widely used to promote postpartum uterine involution for women and our study aimed to evaluate and determine the acute and chronic toxicity effects of Shimshin-6. Aim: To evaluate and substantiate the acute and chronic toxicity effects of Shimshin-6. Materials and Methods: The acute toxicity of Shimshin-6 was evaluated using the rapid method described by V.B. Prozorovsky (1978) by administering intraperitoneal injections of the medicinal extract in white mice to determine the lethal dose. The active dose was determined following the methodology of I.P. Zapadnyuk (1983). Chronic toxicity was evaluated in Wistar rats according to the OECD 407 (2008) guidelines. The test animals were administered Shimshin-6 in tablet form (90 mg/kg and 180 mg/kg) and decoction form (tang) (162 mg/kg) daily for 60 days. At the end of the experiment, biochemical and complete blood analyses were conducted, along with histopathological examination of major organs. The study was conducted with ethical approval granted by the Ethics Committee of the Mongolian National University of Medical Sciences (MNUMS) on October 25, 2024. Results: The LD50 for Shimshin-6 tablets was 4.47 (3.39–5.1) g/kg, indicating low acute toxicity based on the K.K. Sidorov classification. The LD50 for the decoction form was 8.1 (7.1–9.4) g/kg, suggesting it is non-toxic. Regarding chronic toxicity, platelet count was significantly reduced compared to the healthy control group: Shimshin-6 tablet group: 46% reduction at 90 mg/kg and 29.7% reduction at 180 mg/kg. Shimshin-6 decoction group: 60.5% reduction at 162 mg/ kg. Additionally, hemoglobin levels in the decoction group (162 mg/kg) decreased by 15.7% (p<0.05). Biochemical analysis showed a 36.3% reduction in total cholesterol (LDL-C) levels in the tablet group (180 mg/kg) and decoction group (162 mg/kg) compared to the control (p<0.05). Conclusion Shimshin-6 tablets showed low acute toxicity in experimental mice. However, long-term administration may lead to a reduction in platelet count.

Background: Lish-6 has been used for treatment pharyngitis, flu and throat disease. Lish-6 is composed from Eugenia caryophylla. Thumb, Saussurea lappa C.B. Clark, Schizostachoum chinense. Rendle, Glycyrrhiza uralensis. Fisch, Gentiana algida Pall, Terminalia chebula. Retz. Anti-fever properties of these plants and their bio-active compounds have extensively been studied. Aim: To determine the anti-fever effects of Lish-6. Marerials and Methods: Fever was induced by intravenous administration of lipopolysaccharide (LPS) at concentration of 0.5 mg/kg. Lish-6 was given orally at concentration of 92 mg/kg, 1 and 6 hours after the LPS administration. Rectal temperature wa measured 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 hours after the LPS administration. Paracetamoli was given orally at concentration of 50 mg/kg. Blood levels of Prostaglandin E2 (PGE2) and interleukin-6 (IL-6) interleukin-1β (IL-1β) were determined by enzyme linked immune sorbent assay using rat kits. Lung tissue was examined by histopathological analysis. Results: The body temperature of rats in the normal group was 35.0±1.10С, while in the control group, periodic fever was caused by the effect of lipopolysaccharide (p=0.001). But in the Lish-6 drug group, rectal temperature decreased steadily (p=0.05). In addition, the IL-1β cytokine in the normal group was 3.24±0.31 ng/L and increased by 60.5% in the control, indicating the development of the pathological model, while this parameter decreased by 31% in the Lish-6 drug group (p=0.05). IL-6 cytokine in the normal animals was 21.1±0.2 pg/L and increased by 19.04% in the control, indicating the development of the pathological model, while this parameter decreased by 8.3% in the Lish-6 drug group (p =0.05). PGE2 in the normal group was 43.2±0.3 ng/L, and it increased by 62.7% in the control group, indicating the development of a pathological model, while this parameter decreased by 53.3% in the Lish-6 drug group (p=0.05). Conclusion Lish-6 traditional drug has the effect of reducing rectal temperature, IL-1β, PGE2 and IL-6 cytokines during lipopolysaccharide-induced febrile pathology model.

Background: Erkhem-8 medicine is a composed Crocus Sativus L, Lagotis integrifolia, Gentiana barbata froel etc, used for the treatment of respiratory diseases, specially pneumonia and fever or hot disease in Mongolian Traditional Medicine. Aim: The effects of Erkhem-8 medicine to determine the some sourcebook study results. Materials and Methods: An evaluation of its effects on wind, bile, and phlegm was conducted using Traditional Mongolian Medicine methods (Dagvatseren B, 1996). In order to clarify the structure and composition of Erkhem-8 traditional medicines, and the treatment of lung fever from traditional medical sources. Text and source-study and comparison method were used in the study. Results: The study found that Erkhem-8 medicine demonstrated a varying capacity to suppress wind, bile, and phlegm, with bile suppression being the most significant at 0.68, followed by wind suppression at 0.58, and phlegm suppression at 0.16. Erkhem-8 is composed of 8 ingredients, which are safflower Carthamus tinctorius L, gmeline Bostaurus domesticus, Schizostachoum chinense Rendle, wood Santalum album L, Odontitis rubra Baung.Opiz, flora Gentiana decumbens L, herba Lagoti sintegrifolia Schischk, flora Aconitum Kusnezoffii Reichenb. These herbs themselves have a variety of biological activities, including reduce heat, pain relief, anti-oxidant and anti-tumor, anti-inflammation activity. Conclusions 1. The study found that Erkhem-8 exhibited the highest suppression of bile (0.68), followed by wind (0.58), and phlegm (0.16), indicating its effectiveness in modulating these elements. 2. Most of the raw materials included in the composition of Erkhem-8 medicine have a bitter and sweet taste and have the ability to remove heat, especially heat of the solid organs, i.e. heat of the lungs, liver, blood, specially pneumonia and fever or hot disease in Traditional Mongolian Medicine.

Background: Lish-6 has been used for treatment pharyngitis, flu and throat disease. Lish-6 is composed from Eugenia caryophylla Thumb, Saussurea lappa C.B.Clark, Schizostachoum chinense Rendle, Glycyrrhiza uralensis Fisch, Gentiana algida Pall, Terminalia chebula Retz. Anti-fever properties of these plants and their bio-active compounds have extensively been studied. Aim: To determine the pain relief and antibacterial effects of Lish-6. Materials and Methods: To conduct acute toxicity study using V.B. Prozorovsk method. Average lethal dose, lethal and maximum nonlethal doses were determined. Acetic acid (1%-0.1 ml) was injected into the rat abdominal cavity to induce pain. Wistar rat of either sex (n = 6) weighing 18–22g were used. All animals were withdrawn from food 2h before the start of experiment and were divided in five groups. Group I was injected with normal saline (10ml/kg) as control, Diclodenk was given orally at concentration of 25 mg/kg. Group II III, IV, V and VI were injected with Lish-6 was given orally at concentrations of 9.2, 18, 36, 92, 184 mg/kg injection of acetic acid. The number of abdominal constrictions (writhes) were counted of acetic acid injection for the period of 20 min. To determine the antibacterial effect by dilution method. Results: Average lethal dose of Lish-6 was found to be LD50=0.92 (0.6-1.04) g/kg suggesting that it is slightly toxic animals. Control group animals abdominal constrictions 72.4±8.8. Lish-6 concentrations of 9.2, 18, 36, 92, 184 mg/kg group animal reduced acitic acid induced pain by 41.9-78.7% suggesting that it is pain relief effect (p<0.001). 4 g of Lish-6 medicine is active against gram-positive bacteria (S.aureus, Streptococcus pneumonia). However, it is inactive against gram-negative bacteria. In other words, Lish-6 medicine inhibits the growth of methicillin-resistant and non-resistant S.aureus bacteria at a dose of 500 mg. It was also found to inhibit Streptococcus pneumonia at a dose of 250 mg. Conclusion Average lethal dose of Lish-6 was found to be LD50=0.92 (0.6-1.04) g/kg suggesting that it is slightly toxic animal. Lish-6 reduced acitic acid induced pain by 41.9-78.7% suggesting that it is pain relief effect. Lish-6 traditional drug has an antibacterial effect.

Introduction: Diabetes mellitus is a complex, progressive disease, which is accompanied by multiple cardiovascular complications. Oxidative stress is significantly increased in diabetic patients and may lead to great haemostatic disturbances existing in these patients. The major thrust was to review current literature on potential interrelationships between haemostatic and metabolic abnormalities in diabetes mellitus. Zomoshin-6 decoction and Caragana jubata (Fabaceace) used in traditional Mongolian medicine have been shown to have haemostatic and antiviral effects. Objectives: The aim of this study was to examine the effect of Caragana jubata (Pall.) Poir. and Zomoshin-6 decoction on haemostatic parameters and lipid profiles in diabetic rats. Methods: Diabetes was induced in rats by using alloxan at a dose of 150 mg/kg. Control group received distilled water. Caragana jubata (Pall.) Poir and Zomoshin-6 were administered orally at doses of 2.0 g/kg and 0.2 g/kg respectively. Aspirin (100mg/kg) was used for comparison as a standard medicine. All treatments were performed daily for 3 weeks. Blood samples were obtained and analyzed for fibrinogen levels, prothrombin time, triglycerides (TG) and plasma uric acid. Results and conclusions: Levels of TG (p<0.05), glucose (p<0.03) and plasma uric acid (p<0.02) were increased in rats administered alloxan compare to control and these were significantly reduced by Caragana jubata and Zomoshin-6 treatments (Table 1). Caragana jubata and Zomoshin-6 significantly reduced increases in fibrinogen (p<0.05) level and prothrombin time induced by alloxan (Table 2). Table 1. Effect of Caragana jubata (Pall.) Poir and Zomoshin-6 on alloxan-induced increase in levels in TG, uric acid, and glucose. Table 2. Effect of Caragana jubata (Pall.) Poir and Zomoshin-6 on levels of fibrinogen and prothrombin time in rats given alloxan Parameters Control Alloxan 150 mg/kg Alloxan 150 mg/kg Caragana jubata 2.0 g/kg Zomoshin-6 tan 0.2 g/kg Aspirin 100 mg/kg Triglycerde ( mg/dl) 80.9±2.9 139.1±1.7 57±1.8* 64.0±2.5* 75±1.7* Uric acid (mg/dl)1.3±0.4 2.82±0.9 0.85±0.1* 0.9±0.2** 1.1±0.6* Glucose (mg/dl) 110.3±2.8 140±11 99.2±5*** 135.5±22* 125.2±31* Control Alloxan 150 mg/kg Alloxan 150 mg/kg Caragana jubata 2.0 g/kg Zomoshin-6 0.2 g/kg Aspirin 100 mg/kg Fibrinogen (g/l)221.2±8.7 254±3.7 264.3±4.0* 207.4±3.5** 168.4±1.9** Prothrombin time (sec) 14.1±4.6 9.57±0.3* 22.7±2.3* 22.8±0.7** 20.5±3

Background: The global urgency for environmentally friendly technologies has steadily intensified, and as a result, the synthesis of silver nanoparticles with antibacterial properties derived from medicinal plants traditionally used to combat bacterial infections has garnered significant interest. Goals: This research endeavors to synthesize silver nanoparticles utilizing Jidanga and Banzdo plants, widely recognized in traditional medicine, while elucidating their physical, chemical attributes, and biological potential. Methods: The physicochemical characteristics of these silver nanoparticles, synthesized through phytosynthesis, were meticulously examined via various analytical techniques. Ultraviolet and visible light spectrophotometry (UV/Vis), Photon Cross Correlation Spectroscopy (PCCS), Atomic Force Microscope (AFM), Infrared Spectroscopy (FTIR), and X-ray Crystallography (XRD) were employed for comprehensive analysis. Additionally, their antitumor efficacy was assessed via MTT assay utilizing RAW264.7 cell cultures, while antibacterial properties were evaluated through agar diffusion tests against selected bacterial strains. Results: Silver nanoparticles exhibited distinctive UV/Vis absorption peaks at 407-426 nm, affirming their composition. PCCS measurements revealed nanoparticles with hydrodynamic diameters spanning from ~100 nm to ~450 nm, while AFM imaging showcased spherical nanoparticles ranging from ~35 nm to ~97 nm. Crystallography analysis identified both simple cubic and polycrystalline structures. FTIR analysis unveiled the presence of organic compounds adsorbed onto the nanoparticle surfaces, in addition to silver bonds. Notably, the aqueous and ethanolic extracts of Banzdo plants demonstrated dose-dependent inhibition of RAW264.7 cell growth via the MTT assay. Furthermore, the silver nanoparticles exhibited antibacterial activity against a range of test bacteria with inhibition zones spanning 1-4 mm. Conclusion This study successfully synthesized silver nanoparticles utilizing Jidanga and Banzdo plants from traditional medicine, comprehensively characterizing their physicochemical attributes and demonstrating their biological activity. These findings hold promise for the future utilization of phytosynthetically derived silver nanoparticles.