OBJECTIVE: To determine the effect of soy isoflavones on cell proliferation and the transcription levels of follicle-stimulating hormone receptor (FSHR), inhibin α (INHα), INHβB, androgen binding protein (ABP), transferrin (Tf) and vimentin in testis sertoli cells in SD rats. METHODS: Sertoli cells were cultured in vitro, exposed to daidzein at 0.03, 0.3, 3, and 30 μmol/L and genistein at 0.05, 0.5, 5 and 50 μmol/L, respectively. MTT was used to detect the proliferation of sertoli cells. Real-time PCR was used to detect the relative mRNA expressions of FSHR, INHα, INHβB, ABP, Tf and vimentin. RESULTS: Compared with control groups, cell proliferation and the relative mRNA expression levels of INHβB and ABP in the treated cells showed no significant alternation. The INHα mRNA expression levels were increased in 0.3 and 3 μmol/L Dai and 0.05 μmol/L Gen, while the mRNA expression levels of FSHR were downregulated in 30 μmol/L Dai and Gen at all concentrations. Tf mRNA expression levels were downregulated in 30 μmol/L Dai and 5 μmol/L and 50 μmol/L Gen, and the mRNA expression levels of vimentin were downregulated in 3 and 30 μmol/L Dai and 50 μmol/L Gen. CONCLUSION Soy Isoflavones may have potential detrimental effect on the male reproductive system, as they may impact the function of sertoli cells by downregulating the transcription levels of some important proteins.
Androgen-Binding Protein ; metabolism ; Animals ; Inhibin-beta Subunits ; metabolism ; Inhibins ; metabolism ; Isoflavones ; adverse effects ; Male ; RNA, Messenger ; Rats ; Rats, Sprague-Dawley ; Receptors, FSH ; metabolism ; Sertoli Cells ; drug effects ; Soybeans ; chemistry ; Testis ; cytology ; drug effects ; Transferrin ; metabolism

Objective:To evaluate the efficacy of Lanqin oral liquid combined with Azithromycin in treating mycoplasma pneumoniae pneumonia children with syndrome of heat-toxicity blocking lung. Methods:A total of 82 children with mycoplasma pneumoniae from January of 2017 to January of 2019 who met the inclusion criteria were divided into two groups according to the random number table method with 41 patients in each group. The control group was given with Azithromycin sequential therapy, and the observation group was given with Lanqin oral liquid on the basis of conventional western medicine. Both groups were treated for 7 days. Scores of symptoms and signs were evaluated before and after treatment, serum levels of IL-4 and tumor necrosis TNF-α were detected by ELISA method, the disappearance time of fever, cough, dry and wet ralogue and wheezing were observed and recored, adverse reactions occurred during treatment were recorded, and the clinical efficacy was evaluated. Results:The total effective rate of the observation group and the control group was 95.1% (39/41) and 75.6% (31/41), respectively, and the difference was statistically significant ( χ2=4.783, P=0.029). The antipyretic time, cough disappearance time, dry and wet ralogue disappearance time, and wheezing disappearance time of the observation group were significantly shorter than those of the control group ( t=9.312, 8.740, 7.350, 12.467, respectively, all Ps<0.001). After treatment, the scores of cough, fever, wheezing, thirst, phlegm yellow thick and difficultly coughing in the observation group were significantly lower than those in the control group ( t=14.350, 16.779, 18.005, 17.441, 23.633, respectively, all Ps<0.001). After treatment, the scores of cough, fever, wheezing, thirst, yellow thick phlegm and difficultly in coughing in the observation group were significantly lower than those in the control group ( t=14.350, 16.779, 18.005, 17.441, 23.633, respectively, all Ps<0.001). After treatment, serum levels of IL-4 (12.56 ± 1.80 ng/L vs. 17.09 ± 2.78 ng/L, t=8.758) and TNF-α (14.08 ± 2.15 ng/L vs. 20.04 ± 2.93 ng/L, t=10.501) of the observation group were significantly lower than those of the control group ( P<0.01). The incidence of adverse reactions in the control group and the observation group was 17.07% (7/41) and 19.51% (8/41) respectively, and the difference was not statistically significant ( P>0.05). Conclusions:Lanqin oral liquid combined with Azithromycin can improve symptoms of mycoplasma pneumoniae pneumonia children and down-regulate serum levels of IL-6 and TNF-α, increase the efficacy with safety.

Objective : To investigate the co-labeling of c-Fos protein expression and oxytocin ( OXT) neurons in related brain regions in mice injected with dexmedetomidine( Dex) by intraperitoneal injection． Methods : 8 －10 week old C57 mice were divided into Saline group and Dex group．The Saline group was intraperitoneally injected with normal saline，the Dex group was intraperitoneally injected with Dex ( 100 μg / kg) ，the control group was giv- en normal saline (Saline group) ，and the experimental group was given Dex 100 μg / kg (Dex group) ．120 minutes after intraperitoneal injection，the brains of two groups of mice were collected by perfusion，and thenfrozen section and immunofluorescence microscopy imagingwere performed. Results : Immunofluorescence c-Fos protein expres- sion in Dex group and Saline group．The results showed that compared with the Saline group，the Dex group had higher thalamotomy in the supraoptic nucleus ( SON) ，ventrolateral preoptic nucleus ( VLPO) ，subfornical organ (SFO) ，solitary tract (SOL) and area postrema (AP) ，and the difference was statistically significant．C-Fos in the locus coeruleus(LC) ，lateral hypothalamic area (LH) ，laterodorsal tegmental nucleus (LDT) ，parabigeminal nu- cleus (PB) ，ventral tuberomammillary nucleus ( TMN) and motor cortex M1 / M2 protein expression decreased， and the difference was statistically significant (P＜0. 05) ．There was no difference in c-Fos protein expression in parathalamic nucleus (PVN) and suprachiasmatic nucleus ( SCN) ．The results of the co-labeling group of OXT neurons and c-Fos protein in the PVN and SON brain regions showed that : compared with the Saline group，there was no significant difference in the co-labeling of OXT neurons and c-Fos protein in the PVN and SON brain regions of the Dex group． Conclusion The immunofluorescence co-labeling experiment shows that Dex can activate sleep- promoting and other related brain regions，and has a certain inhibitory effect on wake-promoting and exercise-relat- ed brain regions，however，it does not affect the co-labeling of c-Fos protein and OXT neurons in PVN and SON brain regions.