1.Roles of PIF1 helicase in cell cycle arrest induced by ionizing radiation
Zhongqiu LI ; Chao LI ; Degen ZHAO ; Yang YANG ; Xueping LI ; Yan ZENG ; Xiujie PAN ; Zhihua YANG ; Pingkun ZHOU ; Maoxiang ZHU ; Yongqing GU
Military Medical Sciences 2017;41(3):169-173,177
Objective To observe the effect of PIF1 knockdown on cell growth and cell cycle arrest induced by ionizing radiation.Methods HeLa cell lines that consistently down-regulated PIF1 were prepared by the lentivirus granules interfering technology and confirmed by real-time PCR and Western blotting.The effect of down-regulation of PIF1 on cell growth and cell cycle arrest induced by ionizing radiation was evaluated by cell counting and flow cytometry.Results HeLa cell lines consistently down-regulating PIF1 were established.The growth of HeLa that down-regulated PIF1 was inhibited greatly after 4 Gy of γ-ray irradiation.There was little cell proliferation until the 5th day post 4 Gy γ-ray.Moreover, the S phase block and G2/M phase block of PIF1 knock-downed cell lines were significantly delayed after 8 Gy γ-ray irradiation.Conclusion Knockdown of PIF1 can significantly enhance the radiation sensitivity and delayes the S phase block and G 2 /M phase block induced by ionizing radiation.
Result Analysis
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