Objective To investigate the effect of aprotinin on nuclear factor-?B p65 expression in brain regions around the hematoma of intracerebral hamorrage (ICH)in rats. Methods ICH models were induced utilizing the local injection of collagenase into the basal ganglia with sterile technique and divided into ICH group, aprotinin group(ATG) and Pyrrolidine dithiocarbamate(PDTC) group. The expression levels of NF-?B p65 protein and mRNA were detected by immunohisochemistry or RT-PCR at 6 h,24 h,72 h,7 d after injection. Results The amount of NF-?B positive cells increased greatly at 24 h following ICH, peak at 72 h, then decreased in each group. There was no markedly different from ICH group to ATG group, but PDTC group were singnificantly decreased after ICH 24 h~7 d than groups ICH and ATG(P

Objective To explore the curative effects of Edaravone for treatment on Parkinson disease(PD).Methods 30 patients with PD were classified into two groups:18 patients with early PD(courses of disease≤3.5 years) and 12 patients with late PD(courses of disease≥4 years).Edaravone was administered intravenous,30 mg twice daily for 14 d.Then,the drug was discontinued and a follow-up examination carried-out for 3 months.The clinical disability was assessed by using unified PD rating scale(UPDRS) at baseline and 14 d,1 month and 3 months after therapy.The adverse reaction was also observed during the course of treatment.Results In the early PD group,compared to before therapy,the scores of Mentation Behavior and Mood had significantly decreased for 14 d,1 month and 3 months after Edaravone therapy,the score of activities of Daily Living and Motor examination had significantly decreased(P

The drugs promoting axon regeneration after spinal cord injury has been receiving high attention. Growth, extending and branching of neuron axon is a biological process mediated by cytoskeleton, and microtubule plays an important role on axon structure adjustment and growth. Taxol can reasonably stabilize microtubules, eliminate the obstacles of axon regeneration, and effect on axonal regeneration repair after spinal cord injury. Taxol is mainly used for anti-tumor therapy. This paper reviewed the researches on taxel and neuronal cytoskeletal microtubule.

Objective To investigate the level of phosphorylated high-molecular-weight neurofilament (pNF-H) after spinal cord injury (SCI), and explore the relationship between pNF-H and severity of SCI. Methods 20 Sprague-Dawley rats were equally divided into control group (group A), mild SCI group (group B), moderate SCI group (group C) and severe SCI group (group D). The level of pNF-H in serum, BBB score and remaining area of white matter were obtained at different time points. Results The level of serum pNF-H in groups B, C and D arrived at peaks 12 hours and 3 days after SCI, and there was significant difference among them (P<0.05). Both BBB score and remaining area of white matter 14 days after SCI negatively correlated with the level of pNF-H 3 days after SCI (r=-0.987 and r=-0.978, respective-ly). Conclusion The pNF-H increases twice after SCI in rats, and may be associated with the severe of SCI, which can be considered as a biomarker.

Objective To evaluate the effects of celebrity role models on the teaching of rehabilitation medicine. Methods The teaching program of rehabilitation medicine based on the effect of celebrity role models was designed and given to the college students of Capital Medical University School of Rehabilitation Medicine. The students in grades 2014 (group A) and 2015 (group B) were taught by the same teachers with celebrity and general roles, respectively. The learning attitude, immediate test following the teaching and the final exam were carried out to determine the teaching effects. Results The incidence of using mobile phones was higher in group B than in group A (χ2=8.22, P<0.05), and there was no significant difference in the incidence of doze between 2 groups (χ2=2.59, P>0.05). Nobody left early in the class. The scores of the evaluated chapters and the total score in the immediate test were higher in group A than in group B (t>2.048, P<0.05). However, there was no significant difference about those in the final exam (t<1.591, P>0.05). Conclusion The teaching program of rehabili-tation medicine based on the effect of celebrity role models could improve the short-term teaching effect.

Various functional proteins are differently expressed in each pathologic stage after spinal cord injury, representing injury and the pathological change of repair. Ideal biomarker is helpful to evaluate complicated biological reactions of spinal cord injury and predict prognosis. As specific serum markers of central nerve injury, neuron-specific enolase (NSE) and astrocyte primary calcium binding protein S-100β had been studied preliminarily, but the specificity and sensitivity need more research. Future efforts still need to develop ideal biomarkers to predict functional outcome.

Objective To investigate the influence of deep brain stimulation (DBS) at high frequency to the bilateral nucleus accumbens on morphine-induced conditioned place preference (CPP) and relapse behaviors during extinction phase in rats. Methods Twenty adult SD rats were employed in the experiment. Through stereotactic operation, outer electrode cannula was implanted into rats' bilateral nucleus accumbens. After 5 days of rest, the morphine-dependent rat model with CPP was established through intraperitoneal morphine injection (10 mg/kg). The rats, after being randomly divided into experimental group (morphine+DBS) and control group (morphine+sham DBS), were electrically stimulated using DBS circuits. Rats in the experimental group were given high frequency electrical stimulations while the control group was given sham stimulation. The CPP score of the two groups was recorded the day after stimulation until successful extinction and then the extinction time was compared between the two groups. After successful extinction the rats were given small dose of morphine to trigger relapse within 24 hours, and the CPP score was recorded and compared between the two groups.Results Compared with the control group (six days), the experimental group (26 days) had a longer extinction time. After relapse, the retention time within the drug-paired chamber of the experimental group was (357.01±192.72) s, obviously shorter than that of the control group ((704.91±181.35) s;t=2.370, P=0.034 6). Conclusion High frequency DBS to rats' bilateral nucleus accumbens can prolong extinction time but inhibit relapse behavior.

Objective:To analyze the clinical data on hospitalized spinal cord injury patients with calf muscle vein thromboses (MCVTs) seeking prevention and treatment techniques.Methods:The medical records of 423 patients with spinal cord injury were collected. Those with MCVT constituted the observation group, while those without served as controls. Their clinical data were compared.Results:The risk factors for MCVT were screened in logistic regression analyses. The results showed that age, an ASIA grade of A or B, spinal fusion, preventive anticoagulation, physiotherapy treatment and a homocysteine level >15μmol/L were risk factors for the occurrence of a MCVT.Conclusions:Age, an ASIA grade of A or B, spinal fusion or an elevated serum homocysteine level are all risk factors for MCVT. Active anticoagulation and physical therapy may reduce the risk.

Incision of the spinal cord (myelotomy) after spinal cord injury (SCI) is a surgical treatment which is mainly performed within 24 hours after SCI. However, many patients are fi rst seen outside the established time window of 24 hours. Furthermore, little attention has been given to its effi cacy and mechanism after 24 hours. In this study, dorsal longitudinal incision of spinal cord on SCI rats was performed at 72 hours after SCI to remove the liquefi ed necrotic gray matter. The results indicated that after myelotomy, locomotor function of hindlimbs signifi cantly improved from 21st day after SCI. There was also increased residual white matter area at the rostral and caudal segments to the injury center when examined at day 42 after the injury. These results suggested that myelotomy at 72 hours after injury has protective effect on the spinal cord.

Objective To evaluate the quality of life for tuberculosis patients after anterior debridement, autograft bone fusion and anterolateral fixation operation. Methods 17 cases of thoracolumbar spinal tuberculosis were treated surgically from January 2008 to March 2011. All the cases underwent anterior debridement, autograft bone fusion and anterolateral fixation operation. MOS health survey 36-item Short Form (SF-36), Japanese Orthopaedic Association Scores (JOA) and Visual Analogue Scale (VAS) were used to evaluate the quality of life, spine function and pain symptom before and 1 and 6 months after surgery. Results Compared to the results 1 month after surgery, the scores of physical function (PF), role physical (RP), bodily pain (BP), general health (GH), vitality (VT), social function (SF), role emotional (RE), and mental health (MH), and JOA subjective symptoms, JOA clinical signs, and JOA daily activity limitation, and VAS improved (P<0.05) 6 months after surgery; compared to pretreatment, the scores of PF, BP, GH, VT, SF, and MH, and JOA subjective symptoms, JOA clinical signs, and JOA daily activity limitation, and VAS improved (P<0.05). Conclusion The anterior debridement, autograft bone fusion and anterior fixation operation is effective to improve the quality of life, spine function and pain symptom for tuberculosis patients.