Objective To study the chemical constituents of transformed products by Fusarium sacchari and to find the rare ginsenosides with anti-tumor effects from the leaves of Panax notoginseng. Methods The compound isolated was separated by repeated silica gel,Sephadex LH-20,and HPLC chromatography. The structure was identified by analysis of its spectral data.Results A dammarane-type triterpene, notoginsenoside-LZ,20(S)-3?-hydroxy-12?,23-epoxy-dammar-24-ene-20-O-?-D-xylopyranosyl (1→6)-?-D-glucopyranoside,was isolated from the transformed products.Conclusion Notoginsenoside-LZ is a new compound.

Objective To evaluate the effectiveness and safety of recombinant human endostatin combined with concurrent chemoradiotherapy versus concurrent chemoradiotherapy for advanced non small cell lung cancer ( NSCLC) . Methods Electronic databases including the Cochrane library, PubMed, the Chinese biomedical literature database, China national knowledge internet(,EMbase,VIP and Wanfang database system were searched,until August,2013. The inclusion criteria was efficacy and safety studies of randomized controlled clinical studies in which recombinant human endostatin combined with concurrent chemoradiotherapy was compared with concurrent chemoradiotherapy alone for patients with advanced NSCLC. Cochrane handbook 5. 1. 0 was applied in evaluating the quality of included trials and RevMan 5. 1. 0 software was used for data analysis.Results Five studies including 217 cases of advanced NSCLC were included. The results of the meta-analysis exhibited that compared with concurrent chemoradiotherapy alone, recombinant human endostatin combined with concurrent chemoradiotherapy could increase effective rate [OR=2. 62,95%CI(1. 41,4. 86),P=0. 002]. But there were no significant differences in clinical benefit rate [OR=2. 08,95%CI(0. 92,4. 73),P=0. 08],one year survival rate [OR=1. 18,95%CI(0. 53,2. 66),P=0. 68], improvement in quality of life [OR=1. 57,95%CI(0. 40,6. 07),P=0. 52],rate of leucopenia [OR=1. 25,95%CI(0. 72,2. 17), P=0.43],radioactive esophagitis [OR=1. 16,95%CI(0. 42,3. 21),P=0. 77] and radiation pneumonitis [OR=2. 47,95%CI (0. 34,17. 68),P=0. 37]. Conclusion Compared with concurrent chemoradiotherapy alone,recombinant human endostatin combined with concurrent chemoradiotherapy may be more effective for advanced NSCLC,whereas improvement of life quality and toxicities are similar. For the quality restriction and possible publication bias of the included studies,more high quality randomized controlled trials are required to further verify this conclusion.

Objective: To explore the night sleep quality of shift nurses and the current situation of their daytime tiredness, sleepiness, and to provide evidence for nursing administrators and managers to allocate human resources reasonably and prevent adverse events. Methods: The cross-sectional method was utilized to conduct a questionnaire survey among shift nurses in a tertiary teaching hospital in Shandong Province from March to May inclusive, 2017. Results: There was a total of 233 valid questionnaires returned. The prevalence of sleep disorder, daytime tiredness and sleepiness was 45.92%, 16.31% and 13.30%, respectively. The differences of the nurses' sleep quality at night between different ages, marriages, educational backgrounds and professional titles were statistically significant (P<0.05) , while the differences of daytime burnout and sleep state between different shift systems were statistically significant (P<0.01) . Night sleep quality was positively correlated with daytime tiredness and sleepiness (P<0.05) . The results of multiple linear regression analysis showed that age, marriage, educational background and professional title had an impact on nurses' sleep quality at night (P<0.05) . Shift system had an impact on nurses' daytime burnout and sleep apnea (P<0.01) . Conclusion There was a high prevalence of night sleep disorder, daytime tiredness and sleepiness among the shift nurses. Nursing administrators and managers should pay more attention to the night sleep quality of nurses who aged over 30 years old, married, without a bachelor degree and those with a lower professional rank. Furthermore, the current situation of daytime tiredness and sleepiness among two-shift only nurses was worrisome.

Objective To analyze the role of APEX family in hepatocellular carcinoma(HCC).Methods After expression data of HCC and normal liver tissues from public databases(TCGA and TNM plot)were collected,the expression patterns,functional networks,and potential biological pathways of the APEX family in HCC were investigated through differential expression analysis,construction of protein-protein interaction networks(PPI),and Gene Set Enrichment Analysis(GSEA).Receiver operating characteristic(ROC)curve analysis was used to assess the potential of APEX family members as diagnostic markers for HCC,and their correlation with HCC prognosis was anlyzed through survival analysis.RT-qPCR and Western blotting we performed to detect the expression of APEX1 and APEX2 at mRNA and protein levels in liver cells and HCC cells.The effect of silencing APEX1 and APEX2 in HCC cells were determined through CCK-8 assay,EdU assay,scratch healing test,and Transwell assay.Results The expression levels of the 2 members of the APEX family,APEX1 and APEX2,were significantly higher in the HCC samples than the normal liver tissues(P＜0.01).The results of ROC curve analysis on public databases showed that the AUC value of APEX1 and APEX2 in predicting HCC was 0.922 and 0.917,respectively.PPI network analysis revealed that APEX family members regulated cell cycle through various signaling pathways.Survival analysis indicated that HCC patients with high expression levels of APEX1 and APEX2 had significantly shorter overall survival than those with low levels(P＜0.05),and APEX1 and APEX2 were highly correlated with traditional tumor survival prognostic factors Ki-67 and TP53(P＜0.001).The mRNA and protein levels of APEX1 and APEX2 were significantly higher in the liver cancer cells than the normal liver cells(P＜0.05).In addition,silencing APEX1 and APEX2 resulted in notably reduced proliferation,migration,and invasion abilities of HCC cells(P＜0.05).Conclusion APEX1 and APEX2 can serve as potential biomarkers for HCC diagnosis and prognosis assessment,and their involvement in the regulation of HCC proliferation and metastasis provides new strategies and targets for the early diagnosis and individualized treatment of HCC.