0.05).Conclusion: P14ARF and E2F1 can suppress colorectal tumorigenesis.The protein dysfunction is merely an early event in the tumorigenic process,correlated neither with such invasive activities as lymphonode and distant organ metastases nor with clinicopathologic characteristics.P14ARF and E2F1 have a synergenic action in suppressing cell growth and inducing cell apoptosis,and therefore can be used as potentially active gene therapeutic agents for colorectal cancer.

Objective To explore the relationship between MspⅠpolymorphism of CYP1A1 gene and susceptibility to breast cancer in Yi nationality in Yunnan province. Methods The gene polymorphism of restriction enzyme of 3′-terminal of CYP1A1 was detected by PCR-RFLP in 60 healthy Yi women , 51 Yi women with breast cancer, 235 healthy Han women, and 250 Han women with breast cancer. Results The distribution frequency of CYP1A1-MspⅠgenotypes was significantly higher in Yi women with breast cancer (51.0%) than in the healthy Yi women (33.3%) (P < 0.05), an the allele C had a higher frequency in women with breast cancer than in healthy controls (P < 0.05). Significant differences of frequencies were found in genotypes TT, TC and CC between women with breast cancer and healthy controls (P < 0.05). The risks of TC and CC for breast cancer were 1.19 and 1.95 folds respectively to TT genotype. But as compared with Yi women , the distribution frequency of CYP1A1-MspⅠ genotypes did not differ between Han women with breast cancer and in the healthy control (P > 0.05), and there were no differences in three genotype frequencies (P > 0.05). Conclusions Gene polymorphism of CYP1A1 genotypes may be associated with the risk of breast cancer in Yi nationality but not in Han nationality in Yunnan. The mutation of CYP1A1 gene may increase the incidence of breast cancer in Yi nationality.

Objective To study the clinical manifestation, pathological character, treatment strategies and prognostic factors of phyllodes tumor of breast .Methods We retrospectively reviewed 41 patients with breast phyllodes tumor.Results The average age was 35. Among all the patients, there were 20 benign cases, 5 borderline cases, 16 malignant cases. 19 patients received extensive local excision, 7 patients received local excision, 9 patients received simple mastectomy of breast and 6 patients were treated with modified radical operation. There were 4 patients undergoing radiotherapy after the operation. Follow-up was obtained for 35 cases, with an average of 26 months (5~101 months), one of the patients died of metastasis 3 years after the operation, two patients suffered from local recurrence.Conclusions Appropriate operation is the most important prognostic factor of breast phyllodes tumor.

Objective To investigate the risk factors of lymphedema in patients with breast cancer after operation.Methods From January 2012 to May 2016,the clinical data of 350 patients with breast cancer who underwent surgical treatment in First People's Hospital of Foshan were collected.According to the occurrence of postoperative lymphedema,the patients were divided into lymphedema group (87 cases) and non lymphedema group (263 cases).The possible risk factors were evaluated from general demographic indicators,pathological data of breast cancer,perioperative related data and preventive action implementation.Results General demographic data:there was no correlation between the incidence of lymphedema and age,preoperative body mass index (BMI),postoperative BMI,educational level,marital status,residence,family income,menopause,hypertension history (P ＞ 0.05).Pathological data of breast cancer:the occurrence of lymphedema was related with tumor quadrant (x2 =20.039,P ＜ 0.001) and axillary lymph node metastasis (Z =4.713,P ＜ 0.001),but not related with clinical stage and pathological type of tumor (both P≥0.05).Perioperative data:the occurrence of lymphedema was related with the type of incision (x2 =16.921,P ＜ 0.001),axillary lymph node dissection level (x2 =4.433,P ＜ 0.001),the number of axillary lymph node dissection (Z =3.620,P ＜0.001),radiotherapy (x2 =33.290,P ＜ 0.001),endocrine therapy (x2 =10.428,P =0.001),but not related with surgical methods,postoperative complications (subcutaneous fluid,upper limbedema,infection,serum swelling),chemotherapy and neoadjuvant chemotherapy (all P ＞0.05).Comparison of preventive behaviors:avoiding injuries such as cuts and bites (x2 =15.754,P ＜0.001),trimming nails (x2 =9.018,P =0.003),avoiding grasping heavy objects (x2 =11.828,P =0.001) were related with postoperative lymphedema,but other preventive behaviors did not show significant correlation (all P ＞ 0.05).Lymphedema as the dependent variable,multivariate logistic regression analysis results showed that tumor located in the upper outer quadrant (OR =3.943,P ＜ 0.001),surgical incision for longitudinal incision (OR =3.767,P ＜0.001) or oblique incision (OR =2.492,P ＜ 0.001),Ⅱ (OR =1.707,P =0.003) or Ⅲ (OR =4.211,P ＜ 0.001) axillary lymph node dissection,breast/chest wall radiotherapy (OR =2.869,P ＜0.001) or lymph node radiotherapy (OR =4.829,P ＜ 0.001),grabing heavy objects or not (OR =3.264,P ＜ 0.001),avoiding injuries such as cuts and bites (OR =2.602,P ＜ 0.001) were independent risk influence factors of postoperative lymphedema.Conclusion A variety of factors affect the occurrence of postoperative lymphedema in breast cancer patients.Tumors located in the upper outer quadrant,longitudinal or oblique incision,high level of axillary lymph node dissection,radiotherapy,having injuries such as cuts and bites,grabing heavy objects are independent risk influence factors of postoperative lymphedema in breast cancer patients.More attention should be paid to the high risk of lymphedema,and the preventive measures should be taken actively.