ObjectiveBy the study of histological feature of the regenerated nerve at different time after operation,to explore the mechanism of the peripheral nerve regenerationwhen the distal nerve stump is repaired by a smaller proximal end with artery sleeve briding.MethodsFour Wistar rats were served as the donors of arteris for bridging.Sixteen Wistar rats were randomized into 2 groups (n =8 per group).In control group,the right sciatic nerves were cut off and received epineurial suture.In experimental group,the right sciatic nerves received artery sleeve bridging in different size.HE staining was taken to observe the histological changes of the regenerated nerve at 7,14,21 and 28 days after operation.ResultsThe new regenerated axons need about 21 days to pass the bridging gap.Twenty-eight days after operation,the number of the regenerated axons in distal segment was greater than that of the proximal in the experimental group.The regenerated axons were matured and more regular.Conclusion The functional reserve of repaired nerve is exists when the proximal end is smaller than the distal in size by means of amplification effect.And the method of artery sleeve bridging provides a better situation for functional reserve.

0.05) .The total survival rate of N group and H I group was significantly higher than H II group(P0.05) .The abdominal stinking leak was more severe and death rate was higher in N group and H I group than H II group.Conclusion:Dry heat environment in the desert could obviously impair the total survival rate of pigs.

In traditional Chinese medicine, premature ovarian failure is attributed to the menstrual diseases such as amenorrhea, infertility, premenopausal and postmenopausal syndromes. The Zhejiang TCM gynecology schools are good at dialectical treatment of the above menstrual diseases similar to premature ovarian failure, especially Gynecology of Xiaoshan Zhulin Temple, Gynecology of Haining Chen Mushan, Gynecology of Ningbo Song's Family and Gynecology of Hangzhou He's Family. This paper summarizes the characteristics and experience of the four Zhejiang TCM gynecology schools in the dialectical treatment of premature ovarian failure, which can be used for reference.

Objective:To evaluate the safety and efficacy of transplanting beard to scalp using follicular unit extraction(FUE).Methods:Data obtained from hirsute patients with extensive alopecia who underwent hair transplantation between March 2017 and April 2014 at Nanfang Hospital were analyzed. Individual beard hair follicular units (FUs) were harvested under tumescence along the direction of beard growth, using a hollow acute punch with an inner diameter of 0.6-0.7 mm driven with a motor machine. The angle between the punch and the skin is adjusted according to the angle of the beard. The depth of the punch is usually between 2-4 mm and just breaks through the dermis of the skin. The rotation speed of punch is 2 500-3 000 r/min. The harvested FUs were implanted to the bald area. The total amount of FUs harvested and the rate of FUs transection were recorded for each patient. Follow-up examinations were scheduled at 3 days, 10 days, 1 month, 3 months and 9 months postoperatively to check for complications, wound healing and hair growth using portrait photograph and trichoscopy.Results:A total of 58 patients male patients with advanced androgenetic alopecia (AGA) (Norwood-Hamilton Ⅴ-Ⅵ) were included in this study. The average harvested FUs was 2 012±631 and the transection rate for beard FUs was(3.4±0.7)%. The incidence of donor folliculitis was 10.3%(6/58). Folliculitis was cured within 1-3 weeks after treatment. Although all the donor areas were healed normally, 62.1% (36/58) of the patients had remaining mild white spots under the trichoscopy. There was no visible hypopigmented scars observed in the bare areas post-operation. The satisfaction scores of both doctors and patients and the third party were 4.78, 4.40 and 4.76, respectively.Conclusions:Transplanting beard FUs to bald scalp using FUE is a safe and effective for severe androgenetic alopecia with abundant beard.

Objective:To evaluate the safety and efficacy of transplanting beard to scalp using follicular unit extraction(FUE).Methods:Data obtained from hirsute patients with extensive alopecia who underwent hair transplantation between March 2017 and April 2014 at Nanfang Hospital were analyzed. Individual beard hair follicular units (FUs) were harvested under tumescence along the direction of beard growth, using a hollow acute punch with an inner diameter of 0.6-0.7 mm driven with a motor machine. The angle between the punch and the skin is adjusted according to the angle of the beard. The depth of the punch is usually between 2-4 mm and just breaks through the dermis of the skin. The rotation speed of punch is 2 500-3 000 r/min. The harvested FUs were implanted to the bald area. The total amount of FUs harvested and the rate of FUs transection were recorded for each patient. Follow-up examinations were scheduled at 3 days, 10 days, 1 month, 3 months and 9 months postoperatively to check for complications, wound healing and hair growth using portrait photograph and trichoscopy.Results:A total of 58 patients male patients with advanced androgenetic alopecia (AGA) (Norwood-Hamilton Ⅴ-Ⅵ) were included in this study. The average harvested FUs was 2 012±631 and the transection rate for beard FUs was(3.4±0.7)%. The incidence of donor folliculitis was 10.3%(6/58). Folliculitis was cured within 1-3 weeks after treatment. Although all the donor areas were healed normally, 62.1% (36/58) of the patients had remaining mild white spots under the trichoscopy. There was no visible hypopigmented scars observed in the bare areas post-operation. The satisfaction scores of both doctors and patients and the third party were 4.78, 4.40 and 4.76, respectively.Conclusions:Transplanting beard FUs to bald scalp using FUE is a safe and effective for severe androgenetic alopecia with abundant beard.

Objective To explore the effective target and active ingredient prediction of premature ovarian insufficiency(POI)treated by Tiaojing decoction by ferroptosis pathway by network pharmacokogy and bioniformatics.Methods The active ingredients and target genes of Tiaojing decoction were obtained through traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)and bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine databases,disease targets for POI were obtained by using GeneCards,therapeutic target database and Drugbank,and intersection targets were obtained by Venn.Core target gene network of Tiaojing decoction in treatment of POI were constructed through the ferroptosis pathway by ferroptosis database,Cytoscape and STRING software.Then,the DAVID database was used to perform gene ontology and biological pathway enrichment analysis on the predicted targets related to ferroptosis of POI treated with Tiaojing decoction.Subsequently,differential genes in biology related to POI were obtained through the gene expression omnibus(GEO)database for further extraction of key targets,and the binding affinity of active small molecule drug components were verified through molecular docking.Results A total of 154 active ingredients,101 targets related to POI and 23 genes related to ferroptosis were selected from 13 kinds of traditional Chinese medicines in Tiaojing decoction.The enrichment analysis showed that the main involved pathways were phosphatidylinositol-4,5-bisphosphate 3-kinase/protein kinase B(PI3K/Akt)signaling pathway and hypoxia inducible factor-1 signaling pathway.Through GEO database screening,the key targets of Tiaojing decoction in intervening POI through ferroptosis pathway were glycogen synthase kinase-3 β(GSK-3 β),caveolin-1(CAV1),mammalian target of rapamycin(mTOR)and protein kinase C alpha gene(PRKCA),all expression of which reduced in POI.The results of molecular docking showed that CAV1-quercetin and GSK-3β-luteolin had stable binding ability.Conclusion The network pharmacology results suggest that the ferroptosis pathway may be an important mechanism of Tiaojing decoction in treatment of POI,GSK-3β,CAV1,mTOR,PRKCA and other targets,as well as PI3K/Akt signaling pathway,may play important roles in them.