Objective:To study the clinical application of X-ray assisted placement of nasojejunal feeding tube in early enteral nutrition of critical illness. Methods:The feeding tube affiliated wire guide was inserted to jejunum under X-ray observation and affirmanced by the examination with contrast medium. Results:All the feeding tubes were inserted to jejunum successfully.The time was 10-40 minutes,averagely 20 minutes.Conclusion:The placement of the nasojejunal feeding tube under X-ray is simple and safe and offer an effective route of early enteral nutrition for critical illness.

A 300 cm-long ileus tube was inserted from nasal cavity to the jejunum under the radiophotography guidance in 16 patients with recurrent adhesive small-bowel obstruction. Along with entire intestinal decompression, the ileus tube was moved forward to the terminal ileum and remained for 13 to 25 days for internal intubation plication. All the participants improved during the follow-up (range, 8 months to 2 years), and no intestinal adhesion or obstruction occurred. Non-surgical intestinal intubation plication might be a simple, safe and effective treatment for adhesive intestinal obstruction.

Objective To study the relationship of expression of nuclear factor kappab gene and MMP-2mRNA and with lymph node metastasis of pancreatic cancer.Methods The expression of NF-?B was investigated by Western blot in normal pancreatic(NP) samples of 9 cases and pancreatic cancer(PC) samples of 45 cases(lymph node metastasis 30 cases,non-lymph node metastasis 15 cases).The expression of MMP-2mRNA was detected through semiquantitative reverse transcriptase polymerase chain reaction(RT-PCR).Results Of 45 PC cases,the positive expression rate of p65 and MMP-2mRNA was 66.7%(30/45) and(57.7%)(26/45),respectively.Of 30 cases of lymph node metastasis,the positive expression rate of p65 and MMP-2mRNA was 83.3%(25/30)and 73.3 %(22/30),respectively.Of 15 cases of non-lymph node metastasis,the positive expression rate of p65 and MMP-2mRNA is 33.3%(5/15) and 26.7%(4/15),respectively,and the expression of p65 gene was positively correlated with MMP-2mRNA(expression)(r=0.743,P

This paper reviewed the development of the horseback riding exercise machine in Japan based on the theory of therapeutic riding, imitating the saddle movement by using data collected three dimensional from living horse, involving virtual reality technique, 6-axes parallel mechanism and motion capturing technique. It is effective to improve muscular strength and insulin resistance in elderly people. Mechanical horseback riding is hopeful to be used in clinical approach to improve the quality of life in elderly people in the future.

Recent years a large number of researches and studies of modern rehabilitation medicine have made great progress in various fields,such as basic theory,evaluation,treatment,institution,and network.The objective of this paper is to outline these advances,which could be benefit for medical affairs in rehabilitation of cerebrovascular diseases.

.All patients cured.Three-lumen nasogastrojejunal tube placed under X-ray monitoring proves to be a safe, convenient, highly tolerable and effective procedure.

Objective To summarize the experience in diagnosis,prention and management of right accessory hepatic duct(AHD)injury during laparoscopic cholecystectomy(LC).Method The clini-cal data of 8 patients with AHD injury during LC diagnosed and managed retrospectively analyzed. Results Of the 8 patients, 5 patients were found during LC,3 patients were found after LC.Management were ad follow:5 patients with the diameter of the AHD smaller than 3.0 mm were ligated,1 patient with the diameter of 5.0 mm were reconstructed,1 patient with the diameter of 3.0 mm,but the AHD connected with other bile duct in vicinal epaticlobes,were ligated.These 7 patients,with the injured AHD ligated or re-constructed,were followed up for 6 months to 5 years and showed good results.The another who did not be found during LC and not be managed appropriately,arose serious bile leakage and infection for a long time,underwent drainage operations for 3 times,cured after haft year.No death in all patients.Conclusions Correct operative rules should be obeyed strictly in LC,and keep the exist of AHD under close guard,if AHD injury occurred,LC should turn to open surgery immediately,early finding of the injury and managing appropriately is the key to achieve successful result.

Costimulatory molecule CD_(40) is extensively expressed by immune, hematopoietic, epithelial, and a wide range of tumor cells. As a potential target for novel cancer therapy, CD_(40)/CD_(40L) may mediate tumor regression through both an indirect effect of immune activation and a direct cytotoxic effect on the tumor. Several drug formulations that target the CD_(40)/CD_(40L) pathway have undergone phase I clinical evaluation in advanced-stage cancer patients, and initial findings show objective clinical responses immune modulation function and have not serious toxicity.

objective To construct the recombinant adenovirus containing p53 up-regulated modulator of apoptosis(Ad-PUMA)and investigate its growth inhibition effect on pancreatic callCer cells in vitro and in vivo.Methodls Ad-Easy system was used to construct Ad-PUMA by recombination in E.coli.The virus was Dackaged in 293 cells and subsequently identified valid.The AsPC-1 cells were infected with AdPUMA.Before and after Ad-PUMA infection,the expression of PUMA protein wag investigated by western blot,the inhibition rate of AsPC-1 cells was examined by MTY assay.The in vivo tumor suppressive effect was detected in nude mice with human AsPC-1 xenograft.PUMA protein and the apoptosis of AsPC-1 xenograft were detected by western blot and TUNEL(terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling)method.Results In vitro,the expression of PUMA protein was increased with titer of Ad-PUMA,the proliferation of AsPC.1 cells were suppressed,significantly,and the effect was in a viral dose-dependent nlanner.In vivo,the growth in nude mice of AsPC-1 infected with Ad-PUMA was significantly inhibited with an inhibition rate of 44.2%.The expression of PUMA was significantly up-regulated,and the apoptosis index wa8 significantiv increased in tumor after Ad-PUMA infection as determined by western blot and TUNEL.Conclusion The expression of PUMA call inhibit the proliferation of pancreatic cancer in vitro and in vivo,and may be used 88 a potential tool for cancer therapy

Objective:To explore and study the clinical values of CT and MRI in the diagnosis of spinal trauma. Methods: One hundred patients with spinal trauma in our hospital were selected as the study objects and randomly divided them into 2 groups, 50 cases in each group. The control group was diagnosed by CT, the experimental group was diagnosed by MRI. Results: The efficacy of CT(control group) on fracture line and fracture flat shift was better than experimental group, but in the aspect of spinal cord injury, ligamentous injury and soft tissue injury, experimental group(MRI) was more sensitive than control group(x2=4.0, P<0.05), differences between 2 groups were statistically significant. Conclusion:Every coin has 2 sides, so as to applying CT and MRI in the diagnosis of spinal trauma, but the accuracy to overall spinal trauma of experimental group is higher. Furthermore, it can avoid misdiagnosing with spinal trauma that applying combined CT and MRI, it is worthy of being widely recommended in clinic.