Objective To investigate serum angiopoietin-2(Ang-2)levels in non-small cell lung cancer(NSCLC)and its clinical significance.Methods Patients with NSCLC(n=98)and healthycontrols (n=30)were recruited into this study.Serum Ang-2 levels were measured using enzyme-linked immunosorbent assays(ELISA).The results were compared between patients with ifferent tumor stages,metastasis status,and postoperative recurrence 1 year after surgery.Results Patients with NSCLC had higher Ang-2 levels[median 3 230.5 ng/L,range(1 231.6～4 612.5)ng/L ]than the control group [median 831.7 ng/L,range(592.4-1 701.1)ng/L,U=15,P<0.01].Serum Ang-2 levels in stage Ⅲpatients[median 2 311.7 ng/Lrange(1 512.9～3 530.7)ng/L]were significantly higher than stage I [median 1 593.6 ng/L,range(1 235.2～2 763.1)ng/L,H=27.66,P<0.01]and stage Ⅱ patients [median 1 581.4 ng/L,range(1 231.6～2 852.3)ng/L,H=27.66,P<0.01],but significantly lower than stage Ⅳ patients[median 3 808.2 ng/L,range(2 235.8～4 612.5)ng/L,H=65.6P<0.01].Serum Ang-2 levels were higher in patients with distant metastasis or postoperative recurrence than those without them.Conclusion Serum Ang-2 level in patients with NSCLC could be a useful marker for metastasis and postoperative recurrence.

Objective To study the relationship between the dynamic change of free DNA in cerebrospinal fluid (CSF)and the tissue injury in the patients with hypertensive intracerebral hemorrhage.Methods 54 cases of hypertensive intracerebral hemor-rhage were divided into 3 groups according to the glasgow coma scale(including 17 mild cases,21 moderate cases and 16 severe ca-ses).2 mL of CSF was collected for extracting free DNA on 1,3,7,15 d after stroke onset.The free DNA level was measured by the fluorescent real-time PCR.Results The free DNA level in the severe group was significantly higher than that in the mild and mod-erate groups.The free DNA level in the abnormal intracranial pressure group was higher than that in the normal intracranial pres-sure group;the intracranial infection group was higher than the non-infection group.Conclusion The free DNA level has certain value for diagnosing the craniocerebral injury in hypertensive intracerebral hemorrhage and is conducive to monitor the occurrence of postoperative complications.

Objective:To study whether the excitability of facial nerve motor nucleus is increased in patients with facial spasm to further study the mechanism of facial spasm.Methods:Thirty patients with HFS underwent MVD in our hospital from April 2018 and March 2019 were chosen in our study. The amplitude and threshold voltage of facial nerve motor evoked potential (FNMEP) of the affected side and healthy side were monitored and compared by using monopulse stimulation and multi-pulse stimulation. The changes of FNMEP amplitude in patients before and after sevoflurane inhalation were monitored.Results:Stable facial motor evoked potential could be stimulated by monopulse in 26 patients (89.7%) on the affected side, while only 5 patients (17.2%) on the healthy side, showing statistical difference between the two sides ( P<0.05); there was no significant difference in FNMEP amplitude between the affected side and the healthy side ([207.2±62.1] μV vs. [180.2±55.0] μV, P>0.05); the average threshold voltage of neuromotor evoked potential on the affected side was (140.3±26.8) V, which was significantly lower than that in the healthy side ([177.0±23.2] V, P<0.05). The amplitude of FNMEP on the affected side was (207.2±62.1) μV, (133.0±36.5) μV, and (70.4±40.2) μV, respectively, before sevoflurane inhalation, and at 0.5 fold of minimun alveolar concentration (MAC) and 1.0 fold of MAC concentrations, with significant differences ( P<0.05). Conclusion:In patients with hemifacial spasm, there is increased excitability of facial nerve motor nucleus on the affected side, which to a certain extent supports the central theory of hemifacial spasm pathogenesis.

Objective To evaluate the efficacy and safety of nerve combing for trigeminal neuralgia without neurovascular decompression.Methods A retrospective review of clinical data of 281 patients with trigeminal neuralgia,admitted to our hospital from January 2008 to January 2016,was performed.The patients were divided into two groups:patients from group A were treated by nerve combing (n=32),and patients from group B were treated by microvascular decompression (n=249).The two groups were compared in terms of surgical outcomes and postoperative complications.Results The pain was completely relieved in 90.6％ patients of group A (29/32) and 94.4％ patients of group B (235/249),respectively;the mean follow-up duration was 62.5 months in group A,and 78.1％ patients (25/32) were completely relieved;the mean follow-up duration was 59.8 months in group B,and 84.1％ patients (201/239)were completely relieved;no statistically significant differences existed in the surgical outcomes and long-term outcomes between the two groups (P＞0.05).No complications related to severe disability or death were noted in the two groups.The rate of facial numbness in patients from group A (71.9％) was significant higher than that in group B (2.5％,P＜0.05).Conclusions Nerve combing is a safe and effective treatment for trigeminal neuralgia without neurovascular decompression.Most patients treated with nerve combing experienced some degrees of facial numbness.