1.Cloning and Functional Analysis of a Promoter of ?-conglycinin Subunit Gene in Soybean
Xin-Ping YI ; De-Yue YU ;
China Biotechnology 2006;0(10):-
A promoter fragment (7S?P ) of ? subunit gene was isolated by PCR from genomic DNA in various soybean accessions, including cultivars Nannong99-10,N2899,Nannong 88-1, and wild soybeans Jiangpu -1 and ZYD4174.The sequences of 7S?P fragment from these five soybean accessions shared 99% homology, this indicated that the promoter regions of ? subunit gene were conserved. Meanwhile , sequencing analysis showed that the 7S?P fragment contained several seed-specific motifs, such as RY motif, AGCCCA motif, ACGT motif and A/ T rich motif. The expression vector pBI121-7S?P was constructed with the 7S?P fragment (from Nannong99-10) and the GFP reporter gene for functional analysis. Arabidopsis plants were transformed by Agrobacterium mediated method. Southern blot results showed that the 7S?P had been integrated into the genome of Arabidopsis. Assay of GFP expression in the seeds of transgenic Arabidopsis was determined to identify the function of 7S?P promoter. The results showed that 7S?P was a seed-specific promoter.
2.The Review of Assessment of Oocyte Quality
Sheng-Yu XU ; De WU ; Ding-Yue WANG ;
China Biotechnology 2006;0(07):-
The assessments of oocyte quality were usually needed in assisted reproductive technology and reproduction science.Based upon the changes of structure and biochemistry in the development of oocyte,assessments of oocyte quality mainly comprised the oocyte morphology and its maturity.The assessment method of oocyte quality from nondestructive manner and invasive technique to provide multi-pathway for evaluating the oocyte quality reasonable was discussed.
3.Effects of 1,25-Dihydroxyvitamin D_3 on Cell Proliferation,Differentiation and Expression of Vitamin D Receptor in Mouse Osteoblast
hai-yan, GU ; chan-juan, LI ; quan, WANG ; yue, WU ; xi-rong, GUO ; de-yu, ZHAO
Journal of Applied Clinical Pediatrics 2006;0(19):-
Objective To study the effect of different concentration of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] on cell proliferation,differentiation and the expression of vitamin D receptor (VDR) in mouse MC3T3E1 osteoblast.Methods Osteoblast were cultured in medium with different concentrations of 1,25(OH)2D3.Incubated for 48 h,cell proliferation of osteoblast were examined by MTT reduction assay (mono-nuclear cell direc cytotoxicity assay),the osteocalcin (OC) levels in cell medium were detected by ELISA,and the expression of VDR mRNA and protein were examined by using SYBR Green real-time PCR and Western blot,respectively.Results 1.After incubation with 1,25(OH)2D3 for 48 h,the number of MC3T3E1 osteoblast was significantly less than that in control group(P0.05).3.SYBR Green real-time PCR and Western blot results showed that the expression of VDR mRNA as well as VDR protein of osteoblast in 10-8,10-9 mol/L experimental groups were significantly higher than those in control group (Pa0.05).Conclusions Cell proliferation of mouse osteoblast can be inhibited,while the cell differentiation was promoted by 1,25(OH)2D3.1,25(OH)2D3 up-regulated the expression of VDR in mouse osteoblast,which suggested that the VDR signal pathway may play some role in proliferation and differentiation of osteoblast.
4.Identification and characterization of marker chromosome in Turner syndrome
Yue-Qiu TAN ; De-Hua CHENG ; Yu-Fen DI ; Lu-Yun LI ; Guang-Xiu LU ;
Chinese Journal of Obstetrics and Gynecology 2000;0(10):-
Objective To analyze the karyotypes of 11 cases of Turner syndrome with marker chromosome,and study the phenotypic effects resulting from the abnormal karyotype.Methods Eleven Turner syndrome patients had a mosaic karyotype and carried a marker chromosome,and 6 marker chromosomes were ring chromosomes.Their karyotypes were showed as mos.45,X/46,X,+mar or mos. 45,X/46,X,+r.Fluorescence in situ hybridization(FISH)technique with X/Y centromere probes was performed to determine the origin of the marker chromosome.Reverse chromosome painting technique was used to identify the breakpoints of two largest markers.Phenotype effects with different chromosome breakpoints were compared.Results All the 11 marker chromosomes were ring X chromosomes.The breakpoints of the r(X)were involved in Xp22,Xq22,Xq24 and Xq26,etc.Conclusions The marker chromosomes in Turner syndrome mainly originate from X chromosome and form ring chromosome X.Each r (X)in our patients was mosaic,indicating it was originated from mitosis error during early embryo development.To analyze the origin of the marker chromosome and the breakpoint of r(X)will provide guidance for the therapy and prognosis of the Turner syndrome patient.
5.Oral mucosal drug delivery system based on nano technology
Shui-yan CHEN ; Xiao-yu SU ; Xin-min WANG ; Biao LI ; Qing XU ; Peng-fei YUE ; Bao-de SHEN
Acta Pharmaceutica Sinica 2023;58(5):1245-1255
Oral mucosal drug delivery has the advantages of rapid drug absorption, no first-pass effect and good patient compliance. However, factors such as low drug dissolution, saliva carrying the drug into the gastrointestinal tract and the existence of physiological barriers in the mucosa may affect the mucosal permeation and bioavailability of the drug. Nanotechnology applied to drug oral mucosa delivery can overcome the above disadvantages and obtain efficient absorption effect. This paper describes the physiological structure of oral mucosa and the factors affecting the absorption of drugs in oral mucosa, reviews the application of nanotechnology such as liposomes, solid lipid nanoparticles, nanostructured lipid carriers, nanoemulsions, polymer nanoparticles, polymer micelles and nanohybrid suspensions in oral mucosal drug delivery and the mechanism of promoting drug absorption, summarizes the main problems of current research, and gives an outlook on the application of nano oral mucosal drug delivery system. The main problems of current research are summarized, and the prospects for the application of nano oral mucosal drug delivery systems are discussed.
6.Epidemiology of 1968 flu.
Fan YUAN ; Yu LAN ; Jun-Feng GUO ; Xin-Wan LI ; Min-Ju TAN ; Yuan-Ji GUO ; De-Xin LI ; Yue-Long SHU
Chinese Journal of Virology 2009;25 Suppl():33-35
7.Review on the etiological property of 1977 Russian flu virus (H1N1).
Jian-Fang ZHOU ; Lei YANG ; Yu LAN ; Zi LI ; Xiang ZHAO ; Min WANG ; Yuan-Ji GUO ; De-Xin LI ; Yue-Long SHU
Chinese Journal of Virology 2009;25 Suppl():21-22
8.Review on the etiological property of 1968 Hong Kong flu virus (H3N2).
Ning DU ; Xiao-Xing YANG ; Yu LAN ; Le-Ying WEN ; Xiao-Dan LI ; Rong-Bao GAO ; Yuan-Ji GUO ; De-Xin LI ; Yue-Long SHU
Chinese Journal of Virology 2009;25 Suppl():17-20
9.Review on the etiological property of 1957 Asian flu virus (H2N2).
Ning DU ; Xiao-Xing YANG ; Lei YANG ; Yu-Hong ZENG ; Shu-Mei ZOU ; Hong BO ; Yuan-Ji GUO ; De-Xin LI ; Yue-Long SHU
Chinese Journal of Virology 2009;25 Suppl():12-16
10.Review on the etiological property of 1918/1919 Spainsh flu virus (H1N1).
Jian-Fang ZHOU ; Lei YANG ; Yu LAN ; Zi LI ; Xiang ZHAO ; Min WANG ; Yuan-Ji GUO ; De-Xin LI ; Yue-Long SHU
Chinese Journal of Virology 2009;25 Suppl():8-11